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Interspecific relationships of Korean Viola based on RAPD, ISSR and PCR-RFLP analyses  

Yoo, Ki-Oug (Division of Life Sciences, Kangwon National University)
Lee, Woo-Tchul (Division of Life Sciences, Kangwon National University)
Kwon, Oh-Keun (National Horticultural Research Institute, RDA)
Publication Information
Korean Journal of Plant Taxonomy / v.34, no.1, 2004 , pp. 43-61 More about this Journal
Abstract
Molecular taxonomic studies were conducted to evaluate interspecific relationships in Korean Viola 34 taxa including two Japanese populations using RAPD(randornly amplified polymorphic DNA), ISSR(inter simple sequence repeat) and PCR-RFLP(restriction fragment length polymorphism) analysis. Only six and four primers out of 40 arbitrary and 12 ISSR primers were screened for 34 taxa, and were revealed 70 (98.6%) and 28 (96.6%) polymorphic bands, respectively. Fifteen restriction endonucleases produced 80 restriction sites and size variations from the large single copy region of cpDNA, 16 (20%) of which were polymorphic. The separate analyses from the RAPD, ISSR and PCR-RFLP data were incongruent in the relationships among 34 taxa, but combined data was in accordance with previous infrageneric classification system based on morphological characters, especially the subsection and series level. Section Chamaemelanium placed between subsect. Patellares and Vagimtae of section Nomimium was not formed as a distinct group. Viola alb ida complex including three very closely related taxa was recognized independent group within subsect. Patellares in combined data tree. This result strongly suggested that they should be treated to series Pinmtae. RAPD analysis was very useful to clarify the interspecific relationships among the species of Korean Viola than ISSH and PCR-RFLP analyses.
Keywords
Viola; PCR-RFLP; RAPD; ISSR; relationships;
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1 Ballard, H. E., Jr. 1996. Phylogenetic relationships and infrageneric groups in Viola (Violaceae) based on morphology, chromosome number, natural hybridization and Internal Transcribed Spacer(ITS) sequences. Ph.D. dissertation. Univ. of Wisconsin, Madison
2 Ballard, H. E., Jr., K. J. Sytsma. 2000. Evolution and biogeography of the woody Hawaiian violets (Viola, Violaceae): Arctic origins, herbaceous ancestry, and bird dispersal. Evolution 54: 1521-1532   DOI   PUBMED
3 Brainerd, E. 1921. Violets of North America. Vt. Agr. Exp. Sta. Bul. p. 224
4 Clausen, J. 1927. Chromosome number and the relationship of species in the genus Viola. Ann. Bot. 41: 677-714
5 Fritsch, P., M. A. Hanson, C. D. Spore, P. E. Pack and L. H. Reiseberg. 1993. Constancy of RAPD primer amplification strength among distantly related taxa of flowering plants. 11: 10-20   DOI   ScienceOn
6 Gingins, F. 1823. Memoire sur la Famille des Violacees. Mem. Soc. Phy. His. Natur. Geneve. Paschoul, Geneva
7 Hama, E. 1975. The wild violets of Japan in color. Sungmoondang, Tokyo
8 Hashimoto, T. 1967. Violets of Japan. Sungmoondang, Tokyo
9 Nakai, T. 1909. Flora Koreana I. J. Coll. Sci. Imp. Univ. Tokyo 26: 62-74
10 Nakai, T. 1916. Viola coreanae. Bot. Mag. (Tokyo) 30: 276-289
11 Nakai, T. 1922a. Violae Novae Japonicae. Bot. Mag. (Tokyo) 34: 29-39
12 Nakai, T. 1925. Notes sur les Violettes du Japan. Bull. Soc. Bot. (France) 73: 180-195
13 Satake, Y. and E. Ito. 1965. Taxonomic study of the Japanese Viola 2. Chasmogamous flower of the stemless group. Bull. Natl. Sci. Mus., Tokyo 8: 119-237
14 Takenouchi, M. 1955. Brief notes on the taxonomy, ecology and geographic distribution of species of Viola indigenous to Manchuria and Inner-Mongolia. Sci. Contr. Tung-pei Teach. Univ. 5: 65-95
15 Valentine, D. H., H. Merxm?ller and A. Schmidt. 1968. Violaceae. In Florae Europea II. Thulin, T. G., V. H., Heywood, N. A. Burgers, D. M. Moore, D. H. Valentine, S. M. Walter and D. A. Webb (ed.), Cambridge, Pp. 270-282
16 Williams, J. G. K., M. K. Hanafey, J. A. Rafalski and S. V. Tingey. 1993. Genetic analysis using random amplified polymorphic DNA markers. Meth. Enzymol. 218:704-740   DOI   PUBMED
17 김경식. 1986. 한국산 제비꽃속 식물의 비교형태학적 연구. 성균관대학교 박사학위논문. 수원
18 김경식, 선병윤, 황성수, 정국현. 1991. 한국산 제비꽃속의 종분화론적 연구-태백제비꽃군의 비교형태학적 연구. 식물학회지 34: 229-238
19 Clausen, J. 1929. Chromosome number and the relationship of some North American species of Viola. Ann. Bot. 43: 741-764
20 Nadot, S., H. E. Ballard Jr, J. B. Creach and I. Dajoz. 2000. The evolution of pollen heteromorphism in Viola: a phylogenetic approach. Pl. System. Evol. 223: 155-171   DOI   ScienceOn
21 Makino, T. 1902. Observations on the Flora of Japan. Bot. Mag. (Tokyo) 16: 125-142   DOI
22 Nakai, T. 1952. A synoptical sketch of Korean Flora. Bull. Nat. Sci. Mus. 31: 1-152
23 Brainerd, E. 1924. Some natural violet hybrids of North America. Bull. Verm. Agr. Exp. Sta. 239: 1-205
24 Cronquist, A. 1981. An integrated system of classification of flowering plants. Columbia Univ. Press, New York
25 Palibin, J. 1899. Conspectus Florae Koreae I. Acta Horti. Petrop. 17: 30-36
26 Demesure, B., N. Sodzi and R. J. Petit. 1995. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Molec. Ecol. 4: 129-131   DOI   ScienceOn
27 Ishidoya, T. 1929. Review of Viola from Korean and Manshuria. Jour. Chosen Nat. Hist. Soc. 8: 15-17
28 Zietkiewicz, E., A. Rafalski and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183   DOI   ScienceOn
29 Russell, N. 1958c. The nature of Viola missouriensis Greene. Proc. Iowa Acad. Sci. 654: 96-101
30 Yuzepchuk, S. V. 1949. Violaceae. In Flora of the URSS. Shishkin, B. K. and E. G. Bobrov (eds.), Vol. XV. Leningrad Pp. 262-360
31 Kim, C. M. and W. J. Ko. 1980. Chemotaxonomic study on the genus Viola in Jeju Island. J. Plant Biol. 23: 85-90
32 이영로, 이우철. 1968. 제비꽃속 식물의 잎표피와 종자의 형태학적 연구. 생활과학논문집 1: 35-39
33 정태현, 도봉섭, 심학진. 1949. 조선식물명집 I. 초본편. 조선생물학회편. Pp. 85-88
34 Lee, Y. N. 1969. Chromosome numbers of flowering plants in Korea (2). J. Kor. Res. Inst. B.L. 2: 141-145
35 Ballard, H. E., Jr., K. Inoue and K. J. Sytsma. 1998. Phylogenetic relationships and biogeography of Japanese violets (Viola) based on ITS DNA sequences. Amer. J. Bot.(supplement) 85: 108
36 Cortes-Palomec, A. and H. E. Ballard. 2002. Isolation mechanisms and gene flow between co-occurring species of Mexican Viola (Violaceae). 2002 Botany Conference, (abstracts) p. 57
37 Doyle J. J. and J. S. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. 19 : 11-15
38 Russell, N. 1958b. The violets of the Tennessee I. Key to the species and distribution maps. Castanea 23: 63-76
39 이우철, 육창수. 1972. 금강제비꽃 잎의 flavonoid 배당체에 대하여. 식물분류학회지 5: 43-45
40 황성수. 2002. 한국산 제비꽃속 노랑제비꽃절의 분류학적 연구-형태학적 형질을 중심으로. 식물분류학회지 32: 397-416
41 Messinger, W., K. Hummer and A. Liston. 1999. Ribes (Grossulariaceae) phylogeny as indicated by restriction-site polymorphisms of PCR-amplified chloroplast DNA. Pl. Syst. Evol. 217: 185-195   DOI
42 Nakai, T. 1928. Violae ad Floram Japonicum Novae. Bot. Mag. (Tokyo) 42: 556-566   DOI
43 Satake, Y. and E. Ito. 1966a. Taxonomic study of the Japanese Viola 3. Chasmogamous flower of the caulescent group. Bull. Natl. Sci. Mus., Tokyo 9: 247-256
44 Anonymous. 1977. Flora Plantarum Herbacearum Chinae Boreali-Orientalis. Tomus 6. Sci. Publ. Pp. 79-129
45 Chung, T. H. 1959. A key of the species of Violaceae from Korea. J. Plant Biol. 2: 25-26
46 Maekawa, F. and T. Hashimoto. 1963. Violets of Japan : 2. Shibundo-shinko-sha, Tokyo, Pp. 1-9
47 Ballard, H. E., Jr., K. J. Sytsma and R. R. Kowal. 1999. Shrinking the violets: Phylogenetic relationships of infrageneric groups in Viola (Violaceae) based on ITS DNA sequences. Syst. Bot. 23: 439-458   DOI   ScienceOn
48 Russell, N. 1965. Violets (Viola) of central and eastern United States: An introductory survey. SIDA 2: 1-113
49 Russell, N. 1960. Studies in the photoperiodic responses of violets (Viola). The Southwestern Naturalist 5: 177-186   DOI
50 이창복. 1969. 우리나라의 식물자원. 서울대논문집, 농생계. 20: 158-159
51 이우철, 육창수. 1975. 한국산 제비꽃속의 성분에 의한 분류. 식물분류학회지 6: 1-7
52 황성수, 김경식. 1985. 제비꽃속 화분의 형태학적 연구. 전북대 기초과학연구소 논문집 8: 53-59
53 Yoo, K. O., K. J. Malla and J. Wen. 2001. Chloroplast DNA variation of Panax (Araliaceae) in Nepal and its taxonomic implications. Brittonia 53: 447-453   DOI   ScienceOn
54 Maekawa, F. 1954. Violaceae. In Enumeratio Spermatophytarum Japonicum. III. Hara, H. (ed.), Iwanami, Tokyo, Pp. 194-227
55 Satake, Y. and E. Ito. 1966b. Taxonomic study of the Japanese Viola 4. Chasmogamous flower of the caulescent group. Bull. Natl. Sci. Mus., Tokyo 9: 437-455
56 Williams, J. G. K., A. R. K. Kubelik, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531-6535   DOI   ScienceOn
57 Nakai, T. 1911. Flora KoreanaⅡ. J. Coll. Sci. Imp. Univ. Tokyo 31: 445-447
58 Nakai, T. 1922b. Notes on Viola. Bot. Mag. (Tokyo) 36: 52-61, 84-93, 118-121   DOI
59 Lee, Y. N. 1967. Chromosome numbers of flowering plants in Korea(1). J. Kor. Res. Inst. 11: 455-478
60 Becker, W. 1925. Viola. In Engler, A. and K. Prantl, 2nd eds., 21 Bd. Die Naturlichen Pflanzenfamilien, Pp 363-377
61 박만규. 1974. 한국쌍자엽식물지. 정음사, 서울. Pp. 272-279
62 Ballard, H. E., Jr., M. D. Devine and K. J. Sytsma. 1997. Centrifugal allopatric speciation in the Viola canadensis complex (Violaceae) based on ITS DNA sequences. Amer. J. Bot.(supplement) 84: 177
63 Russell, N. H. 1958a. The violets of Minnesota. Proc. Minnesota Acad. Sci. 25-26: 126-191
64 Wang, C. 1991. Reipublicae Popularis Sinicae. Science Press, 51: 1-148
65 Wang, Z., J. L. Weber, G. Zhong and S. D. Tanksley. 1994. Survey of plant short tandem DNA repeats. Theor. Appl. Genet. 88: 1-6
66 Choi, H. K and J. Wen. 2000. A phylogenetic analysis od Panax (Araliaceae): integrating cpDNA restriction site and nrDNA ITS sequence data. Pl. Syst. Evol. 224: 109-120   DOI   ScienceOn
67 Potokina, E., N. Tomooka, D. A. Vaughan, T. Alexandrova and R. -Q. Xu. 1999. Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes. Genet. Resources Crop Evol. 46: 149-161   DOI   ScienceOn
68 Melchior, H. 1925 Die Phylogenetische Entwicklung der Violaceen und die Naturlichen vervantschaftverhaltnisse ihrer gattungen. In Repertorium specierum novarum regni vegetabilis. Beitrage Zur systematik und pflanzengeographie, Vol. 2, Repertoriums, Fedde, F. (ed), Dahlem, Berlin, Pp. 83-125
69 Russell, N. and A. C. Risser. 1960. The hybrid nature of Viola emarginata. Brittonia 12: 298-305   DOI   ScienceOn