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http://dx.doi.org/10.3340/jkns.2016.59.2.98

The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation  

Cho, Hee Kyung (Department of Physical Medicine and Rehabilitation, College of Medicine, Catholic University of Daegu)
Kim, So-Yeon (Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University)
Choi, Mi Jung (Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University)
Baek, Seung Ok (Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University)
Kwak, Sang Gyu (Department of Medical Statistics, College of Medicine, Catholic University of Daegu)
Ahn, Sang Ho (Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University)
Publication Information
Journal of Korean Neurosurgical Society / v.59, no.2, 2016 , pp. 98-105 More about this Journal
Abstract
Objective : Lumbar disc herniation can induce sciatica by mechanical compression and/or chemical irritation. The aim of this study was to compare the effects of GCSB-5 (Shinbaro$^{(R)}$) and NSAIDs on pain-related behavior and on the expressions of microglia, astrocytes, CGRP, TRPV1, IL-6, and CX3CL1 in a rat model of lumbar disc herniation. Methods : 112 male Sprague-Dawley rats underwent implantation of nucleus pulposus to a dorsal root ganglion (DRG). Rats were divided into five groups as follows; a saline group (the vehicle control group) (n=27), a 10 mg/kg aceclofenac group (the aceclofenac group) (n=22), and 100, 300 or 600 mg/kg GCSB-5 groups (the GCSB-5 100, 300, or 600 groups) (n=21 for each group). Rats were tested for mechanical allodynia at 3 days after surgery and at 1 day, 3 days, 7 days, 14 days, 21 days, 28 days, 35 days, 42 days, 49 days, and 56 days after treatment commencement. Immunohistochemical staining of microglia (Iba1), astrocytes (GFAP), CGRP, and TRPV1, and PCR for IL-6 and CX3CL1 were performed on spinal dorsal horns and DRGs at 56 days after medication commencement. Results : After 56 days of GCSB-5 300 administration, mechanical withdrawal thresholds were significantly increased (p<0.05), and immunohistochemical expressions of Iba1, GFAP, CGRP, and TRPV1 were reduced than other groups, but this difference was not statistically significant. Conclusion : These results indicate GCSB-5 reduces mechanical allodynia and downregulates neuroglial activity and the expressions of CGRP and TRPV1 in the spinal segments of a rat model of lumbar disc herniation.
Keywords
GCSB-5; Lumbar disc herniation; Neuropathic pain; Microglia; Astrocytes; Calcitonin gene-related peptide;
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