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http://dx.doi.org/10.3340/jkns.2008.43.4.190

Expression of Cancer-Testis Genes in Brain Tumors  

Lee, Myoung-Hee (Departments of Neurosurgery, Keimyung University School of Medicine)
Son, Eun-Ik (Departments of Neurosurgery, Keimyung University School of Medicine)
Kim, Ealmaan (Departments of Neurosurgery, Keimyung University School of Medicine)
Kim, In-Soo (Departments of Neurosurgery, Keimyung University School of Medicine)
Yim, Man-Bin (Departments of Neurosurgery, Keimyung University School of Medicine)
Kim, Sang-Pyo (Departments of Pathology, Keimyung University School of Medicine)
Publication Information
Journal of Korean Neurosurgical Society / v.43, no.4, 2008 , pp. 190-193 More about this Journal
Abstract
Objective : Cancer-testis (CT) genes are considered promising candidates for immunotherapeutic approaches. The aim of this study was to investigate which CT genes should be targeted in immunotherapy for brain tumors. Methods : We investigated the expression of 6 CT genes (MAGE-E1, SOX-6, SCP-1, SSX-2, SSX-4, and HOMTES-85) using reverse-transcription polymerase chain reaction in 26 meningiomas and 32 other various brain tumor specimens, obtained from the patients during tumor surgery from 2000 to 2005. Results : The most frequently expressed CT genes of meningiomas were MAGE-E1, which were found in 22/26 (85%) meningioma samples, followed by SOX-6 (9/26 or 35%). Glioblastomas were most frequently expressed SOX-6 (6/7 or 86%), MAGE-E1 (5/7 or 71%), followed by SSX-2 (2/7 or 29%) and SCP-1 (1/7 or 14%). However, 4 astrocytomas, 3 anaplastic astrocytomas, and 3 oligodendroglial tumors only expressed MAGE-E1 and SOX-6. Schwannomas also expressed SOX-6 (5/6 or 83%), MAGE-E1 (4/6 or 67%), and SCP-1 (2/6 or 33%). Conclusion : The data presented here suggest that MAGE-E1 and SOX-6 genes are expressed in a high percentage of human central nervous system tumors, which implies the CT genes could be the potential targets of immunotherapy for human central nervous system tumors.
Keywords
Cancer-testis gene; Brain tumor; MAGE-E1; SOX-6; SCP-1; SSX-2; SSX-4; HOM-TES-85;
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1 Chen YT, Scanlan MJ, Sahin U, Türeci O, Gure AO, Tsang S, et al : A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening. Proc Natl Acad Sci U S A 94 : 1914-1918, 1997
2 Chen YT, Scanlan MJ, Venditti CA, Chua R, Theiler G, Stevenson BJ, et al : Identification of cancer/testis-antigen genes by massively parallel signature sequencing. Proc Natl Acad Sci U S A 102 : 7940- 7945, 2005
3 Goodyear O, Piper K, Khan N, Starczynski J, Mahendra P, Pratt G, et al : CD8+ T cells specific for cancer germline gene antigens are found in many patients with multiple myeloma, and their frequency correlates with disease burden. Blood 106 : 4217-4224, 2005   DOI   ScienceOn
4 Gure AO, Türeci O, Sahin U, Tsang S, Scanlan MJ, Jäger E, et al : SSX : a multigene family with several members transcribed in normal testis and human cancer. Int J Cancer 72 : 965-971, 1997   DOI   ScienceOn
5 Jager D, Unkelbach M, Frei C, Bert F, Scanlan MJ, Jager E, et al : Identification of tumor-restricted antigens NY-BR-1, SCP-1, and a new cancer/testis-like antigen NW-BR-3 by serological screening of a testicular library with breast cancer serum. Cancer Immun 2 : 5, 2002   PUBMED
6 Korangy F, Ormandy LA, Bleck JS, Klempnauer J, Wilkens L, Manns MP, et al : Spontaneous tumor-specific humoral and cellular immune responses to NY-ESO-1 in hepatocellular carcinoma. Clin Cancer Res 10 : 4332-4341, 2004   DOI   ScienceOn
7 Lethe B, Lucas S, Michaux L, De Smet C, Godelaine D, Serrano A, et al : LAGE-1, a new gene with tumor specificity. Int J Cancer 76 : 903-908, 1998   DOI   ScienceOn
8 Monji M, Nakatsura T, Senju S, Yoshitake Y, Sawatsubashi M, Shinohara M, et al : Identification of a novel human cancer/testis antigen, KM-HN-1, recognized by cellular and humoral immune responses. Clin Cancer Res 10 : 6047-6057, 2004   DOI   ScienceOn
9 Ono T, Kurashige T, Harada N, Noguchi Y, Saika T, Niikawa N, et al : Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen. Proc Natl Acad Sci U S A 98 : 3282-3287, 2001
10 Scanlan MJ, Gordon CM, Williamson B, Lee SY, Chen YT, Stockert E, et al : Identification of cancer/testis genes by database mining and mRNA expression analysis. Int J Cancer 98 : 485-492, 2002   DOI   ScienceOn
11 Simpson AJ, Caballero OL, Jungbluth A, Chen YT, Old LJ : Cancer/testis antigens, gametogenesis and cancer. Nat Rev Cancer 5 : 615-625, 2005   DOI   ScienceOn
12 Wang Z, Zhang Y, Mandal A, Zhang J, Giles FJ, Herr JC, et al : The spermatozoa protein, SLLP1, is a novel cancer-testis antigen in hematologic malignancies. Clin Cancer Res 10 : 6544-6550, 2004   DOI   ScienceOn
13 Zendman AJ, Cornelissen IM, Weidle UH, Ruiter DJ, van Muijen GN : CTp11, a novel member of the family of human cancer/testis antigens. Cancer Res 59 : 6223-6229, 1999   PUBMED
14 Scanlan MJ, Simpson AJ, Old LJ : The cancer/testis genes : review, standardization, and commentary. Cancer Immun 4 : 1, 2004   PUBMED
15 Sahin U, Koslowski M, Tureci O, Eberle T, Zwick C, Romeike B, et al : Expression of cancer testis genes in human brain tumors. Clin Cancer Res 6 : 3916-3922, 2000   PUBMED
16 Ueda R, Iizuka Y, Yoshida K, Kawase T, Kawakami Y, Toda M : Identification of a human glioma antigen, SOX6, recognized by patients' sera. Oncogene 23 : 1420-1427, 2004   DOI   ScienceOn
17 Zendman AJ, Ruiter DJ, Van Muijen GN : Cancer/testis-associated genes : identification, expression profile, and putative function. J Cell Physiol 194 : 272-288, 2003   DOI   ScienceOn
18 Boon T, van der Bruggen P : Human tumor antigens recognized by T lymphocytes. J Exp Med 183 : 725-729, 1996   DOI
19 Tureci O, Sahin U, Zwick C, Koslowski M, Seitz G, Pfreundschuh M : Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens. Proc Natl Acad Sci U S A 95 : 5211-5216, 1998
20 Brinkmann U, Vasmatzis G, Lee B, Pastan I : Novel genes in the PAGE and GAGE family of tumor antigens found by homology walking in the dbEST database. Cancer Res 59 : 1445-1448, 1999   PUBMED
21 Sasaki M, Nakahira K, Kawano Y, Katakura H, Yoshimine T, Shimizu K, et al : MAGE-E1, a new member of the melanomaassociated antigen gene family and its expression in human glioma. Cancer Res 61 : 4809-4814, 2001   PUBMED
22 Ueda R, Yoshida K, Kawakami Y, Kawase T, Toda M : Immunohistochemical analysis of SOX6 expression in human brain tumors. Brain Tumor Pathol 21 : 117-120, 2004   DOI
23 Gure AO, Wei IJ, Old LJ, Chen YT : The SSX gene family : characterization of 9 complete genes. Int J Cancer 101 : 448-453, 2002   DOI   ScienceOn
24 van Baren N, Bonnet MC, Dreno B, Khammari A, Dorval T, Piperno-Neumann S, et al : Tumoral and immunologic response after vaccination of melanoma patients with an ALVAC virus encoding MAGE antigens recognized by T cells. J Clin Oncol 23 : 9008-9021, 2005   DOI   ScienceOn
25 van den Eynde B, Peeters O, De Backer O, Gaugler B, Lucas S, Boon T : A new family of genes coding for an antigen recognized by autologous cytolytic T lymphocytes on a human melanoma. J Exp Med 182 : 689-698, 1995   DOI
26 Feller AJ, Duan Z, Penson R, Toh HC, Seiden MV : TRAG-3, a novel cancer/testis antigen, is overexpressed in the majority of melanoma cell lines and malignant melanoma. Anticancer Res 20 : 4147-4151, 2000   PUBMED
27 Boel P, Wildmann C, Sensi ML, Brasseur R, Renauld JC, Coulie P, et al : BAGE : a new gene encoding an antigen recognized on human melanomas by cytolytic T lymphocytes. Immunity 2 : 167-175, 1995   DOI   ScienceOn
28 Tureci O, Sahin U, Schobert I, Koslowski M, Scmitt H, Schild HJ, et al : The SSX-2 gene, which is involved in the t (X;18) translocation of synovial sarcomas, codes for the human tumor antigen HOMMEL- 40. Cancer Res 56 : 4766-4772, 1996   PUBMED