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http://dx.doi.org/10.12750/JET.2018.33.3.107

The Effect Estrogen and Testosterone on the Expression of Melanogenesis-related Genes in Korean Brindle Cattle  

Seo, Hui-Gyeong (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University)
Lee, Ji-Hye (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University)
Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University)
Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University)
Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
Publication Information
Journal of Embryo Transfer / v.33, no.3, 2018 , pp. 107-117 More about this Journal
Abstract
The purpose of this study is to expression pattern of melanogenesis associate genes on cultured melanocyte layer cells in Korean Brindle Cattle(Dark, Brindle and Yellow) were analyzed to evaluate the effects of sex hormones on the control of melanogenesis pathways. Korean Brindle Cattle(Dark, Brindle and Yellow) melanocyte in the skin cells was collected. after the addition of estrogen and testosterone, the culture was analyzed for expression of cell activity and melanin genes for 72 hours. For the analysis of estrogen in different coat color other than the melanogenesis-related genes it is increasingly yellow showed low expression. in particular, the cells of the brindle coat color is low active and expression of genes. However, the testosterone was low, the expression of cell activity inhibiting MMP-2. the expression of melanin genes actually showed a tendency to increase gradually, which is testosterone compared with the estrogen to be considered that affect the skin cell layer brindle coat color. In this study, stimulation with estrogen triggered the inhibition of MC1R of the melanocyte in brindle coat color, but testosterone is induced MC1R in melanocyte. Therefore, considered the eumelanin or phaeomelanin activation are controlled caused by differential expression of sex hormones on melanocyte in Korean Brindle Cattle.
Keywords
hormone; Korean brindle cattle; MC1R; melano;
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1 Girardot M, Guibert S, Laforet M-P, Gallard Y, Larroque H and Oulmouden A. 2006. The insertion of a full-length Bos taurus LINE element is responsible for a transcriptional deregulation of the Normanne Agouti gene. Pigment Cell Research 19:346-355.   DOI
2 Guibert S, Girardot M, Leveziel H, Julien R and Oulmouden A. 2004. Pheomelanin coat colour dilution in French cattle breeds is not correlated with the TYR, TYRP1 and DCT transcription levels. Pigment Cell Res. 17:337-345.   DOI
3 Ito S, Wakamatsu K and Ozeki H. 2000. Chemical analysis of melanins and its application to the study of the regulation of melanogenesis. Pigment Cell Res. 13 Suppl. 8:103-109.   DOI
4 Jackson IJ. 1993. Colour-coded switches. Nature 362:589-589.   DOI
5 Jackson IJ. 1994. Molecular and developmental genetics of mouse coat color. Annu. Rev. Genet. 28:189-217.   DOI
6 Joerg H, Fries HR, Meiferink E and Stranzinger GF. 1996. Red coat color in Holstein cattle is associated with a deletion in the MSHR gene. Mamm. Genome 7:317-318.   DOI
7 Kim SH, Hwang SY and Yoon JT. 2014. Microarray-Based Analysis of the Differntial Expression of Melanin Synthesis Genes in Dark and Light-Muzzle Korean Cattle. PLOS ONE 9:e96453.   DOI
8 Klungland H, Vage DI, Gomez-Raya L, Adalsteinsson S and Lien S. 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm. Genome 6:636-639.   DOI
9 Parrish JA, Jaenicke KF and Anderson RR. 1982. Erythema And Melanogenesis Action Spectra Of Normal Human Skin. Photochemistry and Photobiology 36:187-191.   DOI
10 Robbins LS, Nadeau JH, Johnson KR, Kelly MA, Roselli-Rehfuss L, Baack E, Mountjoy KG and Cone RD. 1993. Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function. Cell 72:827-834.   DOI
11 Rouzaud F, Martin J, Gallet PF, Delourme D, Goulemot-Leger V, Amigues Y, Menissier F, Leveziel H, Julien R and Oulmouden A. 2000. A first genotyping assay of french cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (MC1R). Genet. Sel. Evol. 32:511-520.   DOI
12 Royo LJ, Alvarez I, Fernandez I, Arranz JJ, Gomez E and Goyache F. 2005. The coding sequence of the ASIP gene is identical in nine wild-type coloured cattle breeds. J. Anim. Breed Genet. 122:357-360.   DOI
13 Selz Y, Braasch I, Hoffmann C, Schmidt C, Schultheis C, Schartl M and Volff JN. 2007. Evolution of melanocortin receptors in teleost fish : the melanocortin type 1 receptor. Gene 401:114-122.   DOI
14 Shen T, Heo SI and Wang MH. 2012. Involvement of the p38 MAPK and ERK signaling pathway in the anti-melanogenic effect of methyl 3,5-dicaffeoyl quinate in B16F10 mouse melanoma cells. Chem. Biol. Interact. 199:106-111.   DOI
15 Slominski A, Tobin DJ, Shibahara S and Wortsman J. 2004. Melanin pigmentation in mammalian skin and its hormonal. regulation Physiol. Rev. 84:1155-1228.   DOI
16 Vage DI, Klungland H, Lu D and Cone RD. 1999. Molecular and pharmacological characterization of dominant black coat color in sheep. Mamm. Genome 10:39-43.   DOI
17 한상기, 정의룡, 양교석, 신유철. 1991. 한우개량을 위한 유단백질의 유전적 다형현상에 관한 연구. 한국축산학회지 33:111-120.
18 Wang N and Hebert DN. 2006. Tyrosinase maturation through the mammalian secretory pathway: bringing color to life. Pigment Cell Res. 19:3-18.   DOI
19 Zhou J, Shang J, Ping F and Zhao G. 2012. Alcohol extract from Vernonia anthelmintica (L) willd seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocyte. J. Ethnopharmacol. 143:639-647.   DOI
20 이호준, 홍연식, 이경태, 김상환, 정덕원, 정경섭, 김경래, 민관식, 윤종택. 2008. 칡소 수정란 이식 한우의 임신기간, 생시체중 및 모색발현 양상 조사. 한국수정란이식학회지 23:69.
21 Costin GE and Hearing VJ. 2007. Human skin pigmentation: melanocytes modulate skin color in response to stress. FASEB J. 21:976-994.   DOI
22 Agar N and Young AR. 2005. Melanogenesis: a photoprotective response to DNA damage?. Mutation Research 571:121-132.   DOI
23 Bischitz PG and Snell RS. 1959. The Effect of Testosterone on the Melanocytes and Melanin in the Skin of the Intact and Orchidectomised Male Guinea-Pig. The Journal of Investigative Dermatology 33:299-306.   DOI
24 Chung ER, Kim WT, Kim YS and Han SK. 2000. Identification of Hanwoo meat using PCR-RFLP of MC1R gene associated with bovine coat color. Kor. J. Anim. Sci. Technol. 42:379-390.
25 Deng WD, Shu W, Yang SL, Shi XW and Mao HM. 2009. Pigmentation in Black-boned sheep (Ovis aries): association with polymorphism of the MC1R gene. Mol. Biol. Rep. 36:431-436.   DOI
26 Giradot M, Martin J, Guibert S, Leveziel H, Julien R and Oulmouden A. 2005. Widespread expression of the bovine Agouti gene results from at least three alternative promoters. Pigment Cell Res. 18:31-41.