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http://dx.doi.org/10.12750/JET.2017.32.3.139

Effects of Fructose in a Chemically Defined Maturation Medium on Oocyte Maturation and Parthenogenetic Embryo Development in Pigs  

Shin, Hyeji (College of Veterinary Medicine, Kangwon National University)
Kim, Minji (Division of Applied Animal Science, College of Animal Life Science, Kangwon National University)
Lee, Joohyeong (Institute of Veterinary Science, Kangwon National University)
Lee, Seung Tae (Division of Applied Animal Science, College of Animal Life Science, Kangwon National University)
Park, Choon-Keun (Division of Applied Animal Science, College of Animal Life Science, Kangwon National University)
Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University)
Lee, Eunsong (College of Veterinary Medicine, Kangwon National University)
Publication Information
Journal of Embryo Transfer / v.32, no.3, 2017 , pp. 139-146 More about this Journal
Abstract
The objective of this study was to determine the effect of fructose that was supplemented to a chemically defined in vitro maturation (IVM) medium on oocyte maturation and embryonic development after parthenogenesis in pigs. The base medium for in vitro maturation (IVM) was porcine zygote medium (PZM) that was supplemented with 0.05% (w/v) polyvinyl alcohol (PVA) or 10% (v/v) porcine follicular fluid (pFF). In the first experiment, when immature pig oocytes were matured in a chemically defined medium that was supplemented with 5.5 mM glucose or with 1.5, 3.0 and 5.5 mM fructose, 3.0 mM fructose resulted in a higher nuclear maturation (91.5%) than 1.5 and 5.5 mM fructose (81.9 and 81.9%, respectively) but showed a similar result with 5.5 mM glucose (94.2%). However, there was no significant differences among groups in the embryo cleavage (89.4-92.4%), blastocyst formation (37.5-41.1%), and mean cell number of blastocyst (30.8-34.2 cells). Fructose at the concentration of 3.0 mM (1.08 pixels/oocyte) resulted in a higher intra-oocyte glutathione (GSH) content than 1.5 and 5.5 mM fructose (1.00 and 0.87 pixels/oocytes, respectively) while the cumulus cell expansion was not influenced. In the second experiment, effect of individual and combined supplementation of a chemically defined maturation medium with 5.5 mM glucose or 3.0 mM fructose was examined. No significant effect was found in the nuclear maturation (86.3-92.6%). Embryo cleavage was significantly increased by the combined supplementation with glucose and fructose (95.2%) compared to that with 3.0 mM fructose only (85.7%) while blastocyst formation (37.3-42.8%) and embryonic cell number (33.3-34.1 cells) were not altered. Effect of supplementation of pFF-containing medium with glucose and fructose + glucose was examined in the third experiment. No significant effect by the supplementation with glucose and fructose or glucose alone was observed in the nuclear maturation of oocytes (90.7-94.1%) and blastocyst formation (51.0-56.5%). Our results demonstrate that 3.0 mM fructose was comparable to 5.5 mM glucose in supporting in vitro oocyte maturation and embryonic development after parthenogenesis and could be used as an alternative energy source to glucose for in vitro maturation of pig oocytes.
Keywords
fructose; oocyte maturation; parthenogenesis; defined medium; pig;
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1 Abeydeera LR, Wang WH, Prather RS and Day BN. 1998. Maturation in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro. Biol. Reprod. 58:1316-1320.   DOI
2 Aitken RJ. 1976. Uterine secretion of fructose in the red deer. J. Reprod. Fertil. 46:439-440.   DOI
3 Brad AM, Herrick JR, Lane M, Gardner DK and Krisher RL. 2003. Glucose and lactate concentrations affect the metabolism of in vitro matured porcine oocytes. Purdue University Swine Res. Rep. 136.
4 Duque P, Hidalgo CO, Gomez E, Pintado B, Facala E and Diez C. Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality. Reprod. Nutri, Dev. 43:487-496.
5 De Matos DG and Furnus CC. 2000. The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development: effect of bmercaptoethanol, cysteine and cystine. Theriogenology 53:761-771.   DOI
6 Downs SM and Utecht AM. 1999. Metabolism of radiolabeled glucose by mouse oocytes and oocyte-cumulus cell complexes. Biol. Reprod. 60:1446-1452.   DOI
7 Downs SM and Mastropolo AM. 1994. The participation of energy substrates in the control of meiotic maturation in murine oocytes. Dev. Biol. 162:154-168.   DOI
8 Eppig JJ. 1982. The relationship between cumulus cell-oocyte coupling, oocyte meiotic maturation, and cumulus expansion. Dev. Biol. 89:268-272.   DOI
9 Flood MR and Wiebold JL. 1988. Glucose metabolism by preimplantation pig embryos. J. Reprod. Fertil. 84:7-12.   DOI
10 Gardner DK. 1998. Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. Theriogenology 49:83-102.   DOI
11 Guyader-Joly C, Khatchadourian C and Menezo Y. 1996. Comparative glucose and fructose incorporation and conversion by in vitro produced bovine embryos. Zygote 4:85-91.   DOI
12 Kikuchi K, Onishi A, Kashiwazaki N, Iwamoto M, Noguchi J and Kaneko H. 2002. Successful piglet production after transfer of blastocysts produced by a modified in vitro system. Biol. Reprod. 66:1033-1041.   DOI
13 Hashimoto S, Minami N, Yamada M and Imai H. 2000. Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization relevance to intracellular reactive oxygen species and glutathione contents. Mol. Reprod. Dev. 56:520-526.   DOI
14 Haynes NB and Lamming GE. 1967. The carbohydrate content of sow uterine flushings. J. Reprod. Fertil. 14:335-337.   DOI
15 Hong J and Lee E. 2007. Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte maturation, fertilization, and preimplantation development. Theriogenology 68:728-735.   DOI
16 Kim J, You J, Hyun SH, Lee G, Lim J and Lee E. 2010. Developmental competence of morphologically poor oocytes in relation to follicular size and oocyte diameter in the pig. Mol. Reprod. Dev. 77:330-339.
17 King, N., Korolchuk, S., McGivan, J., and Suleiman, M. 2004. A new method of quantifying glutathione levels in freshly isolated singlesuperfused rat cardiomyocytes. J. Pharmacol. Toxicol. Methods 50:215-222.   DOI
18 Kwun J, Chang K, Lim J, Lee E, Lee B and Kang S. 2003. Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: improved blastocyst formation after glucose replacement with fructose in a serum-free culture medium. Mol. Reprod. Dev. 65:167-174.   DOI
19 Ludwig TE, Lane M and Bavister BD. 2001. Differential effect of hexoses on hamster embryo development in culture. Biol. Reprod. 64:1366-1374.   DOI
20 Lee ES, Fukui Y, Lee BC, Lim JM, and Hwang WS. 2004. Promoting effect of amino acids added to a chemically defined medium on blastocyst formation and blastomere proliferation of bovine embryos cultured in vitro. Anim. Reprod. Sci. 84:257-267.   DOI
21 Marei WF, Ghafari F and Fouladi-Nashta AA. 2012. Role of hyaluronic acid in maturation and further early embryo development of bovine oocytes. Theriogenology 78:670-677.   DOI
22 Moley KH. 2001. Hyperglycemia and apoptosis: mechanisms for congenital malformations and pregnancy loss in diabetic women. Trends Endocrinol. Metab. 12:78-82.   DOI
23 Rieger D, and Loskutoff NM. 1994. Changes in the metabolism of glucose, pyruvate, glutamine and glycine during maturation of cattle oocytes in vitro. J. Reprod. Fertil. 100:257-262.   DOI
24 Sakatani M, Suda I, Oki T, Kobayashi S, Kobayashi S and Takahashi M. 2007. Effects of purple sweet potato anthocyanins on development and intracellular redox status of bovine preimplantation embryos exposed to heat shock. J. Reprod. Dev. 53:605-614.   DOI
25 Sakkas D, Urner F, Menezo Y and Leppens G. 1993. Effects of glucose and fructose on fertilization, cleavage, and viability of mouse embryos in vitro. Biol. Reprod. 49:1288-1292.   DOI
26 Styler L. 1995. Biochemistry. New York: WH Freeman and Co.
27 Yong HY and Lee E. 2007. Presence of intact cumulus cells during in vitro fertilization inhibits sperm penetration but improves blastocyst formation in vitro. J. Emb. Trans. 22:1-7 (in Korean).
28 Vanderhyden BC, Caron PJ, Buccione R and Eppig JJ. 1990. Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation. Dev. Biol. 140:307-317.   DOI
29 Wiesak T, Hunter MG and Foxcroft GR. 1990. Differences in follicular morphology, steroidogenesis and oocyte maturation in naturally cyclic and PMSG/hCG-treated prepubertal gilts. J. Reprod. Fertil. 89:633-641.   DOI
30 Yamauchi N and Nagai T. 1999. Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol. Reprod. 61:828-833.   DOI
31 Zhang L, Jiang S, Wozniak PJ, Yang X and Godke RA. 1995. Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro. Mol. Reprod. Dev. 40:338-344.   DOI