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http://dx.doi.org/10.12750/JET.2016.31.3.145

Effects of antioxidants on viability, plasma membrane integrity and apoptosis in porcine ovarian granulosa cells damaged by bromopropane  

Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University)
Park, Hee-Woo (College of Animal Life Sciences, Kangwon National University)
Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University)
Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Journal of Embryo Transfer / v.31, no.3, 2016 , pp. 145-151 More about this Journal
Abstract
The purpose of this study was to examine the effects of taurine and vitamin E on ovarian granulosa cells damaged by bromopropane (BP) in pigs. We evaluated cell viability, plasma membrane integrity (PMI) and apoptotic morphological change in porcine ovarian granulosa cells. The cells were treated with 1-BP (0, 5.0, 10, and $50{\mu}M$), 2-BP (0, 5.0, 10, and 50 mM), taurine (0, 5.0, 10, and 25 mM), and vitamin E (0, 100, 200, and $400{\mu}M$) for 24 h. $10{\mu}M$ 1-BP and $50{\mu}M$ 2-BP inhibited viability and PMI, and induced apoptosis in porcine ovarian granulosa cells (p < 0.05). Cell viability and PMI were increased by taurine (10 and 25 mM) and vitamin E (100 and $200{\mu}M$), and apoptosis decreased (p < 0.05). Finally, the porcine ovarian granulosa cells were co-treated with BPs ($10{\mu}M$), taurine (10 mM) and/or vitamin E ($200{\mu}M$). Cell viability and PMI in the co-treated cells were increased, and apoptosis was decreased. In conclusion, taurine and vitamin E can improve cell viability and inhibition of apoptosis in porcine ovarian granulosa cells damaged by bromopropane.
Keywords
bromopropane; taurine; vitamin E; viability; ovarian granulosa cell; porcine;
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