Browse > Article
http://dx.doi.org/10.12750/JET.2014.29.3.297

Coat Color of Korean Brindle Cattle and Melanocortin 1 Receptor (MC1R) mRNA: Variation of 3'-Untranslated Region and Expression  

Lee, Hae-Lee (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research)
Park, Jae-Hee (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research)
Kim, Jong Gug (Department of Animal Sciences and Institute of Rare Earth for Biological Application, College of Agriculture and Life Science, Chonbuk National University)
Publication Information
Journal of Embryo Transfer / v.29, no.3, 2014 , pp. 297-303 More about this Journal
Abstract
The objective of this study was to determine the breed differences in the 3'-untranslated region (UTR) of MC1R mRNA, which may be used to distinguish Korean brindle cattle (Chikso) from other breeds. We investigated the relationship between the variation of 3'-UTR of the MC1R mRNA and coat color among different breeds and the Korean brindle cattle with different coat colors. MC1R mRNA expression levels were determined in accordance with the coat color and hair colors of the tail. Total cellular RNA was extracted from the hair follicles of the tails in Hanwoo, Korean brindle cattle, Holstein and $Hanwoo{\times}Holstein$ crossbred cattle. After cDNA synthesis, PCR was performed. Sequences of the 3'-UTR of MC1R mRNA were analyzed. The 3'-UTR of the MC1R mRNA from different breeds of cattle did not show any variations. There were no variations in the 3'-UTR of the MC1R mRNA in Korean brindle cattle with different coat colors. The levels of MC1R mRNA expression in hair follicles of the tail varied substantially among the Korean brindle cattle with different coat colors, except yellow coat color. Correlation between the MC1R mRNA expression in the hair follicles of the tail and coat color may be present in the Korean brindle cattle, but not between the variations of 3'-UTR of MC1R mRNA and coat color. Further studies to determine the regulation of MC1R mRNA expression from the hair follicles of different coat colors will be beneficial in clarifying the role of MC1R in the coat colors of the Korean brindle cattle.
Keywords
Korean brindle cattle (Chikso); MC1R; mRNA; 3'-untranslated regions (UTRs); expression levels;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Barsh GS. 1996. The genetics of pigmentation: from fancy genes to complex traits. Trends Genet. 12(8):299-305.   DOI   ScienceOn
2 Barsh GS. 2001. Coat Colour Mutations, Animals. Academic Press, New York.
3 Berryers TG, Schmutz SM, Schimpf RJ, Cowan CM and Potter J. 2003. TYRP1 is associated with dun coat colour in Dexter Cattle or how now brown cow? Anim. Genet. 34: 169-175.   DOI   ScienceOn
4 Brenig B, Beck J, Floren C, Bornemann-Kolatzki K, Wiedemann I, Hennecke S, Swalve H and Schutz E. 2013. Molecular genetics of coat colour variations in White Galloway and White Park cattle. Anim. Genet. 44(4):450-453.   DOI   ScienceOn
5 Charlier C, Denys B, Belanche JI, Coppieters W, Grobet L, Mni M, Womack J, Hanset R and Georges M. 1996. Microsatellite mapping of the bovine roan locus-a major determinant of White Heifer disease. Mamm. Genome 7(2):138-142.
6 Decker CJ and Parker R. 1995. Diversity of cytoplasmic functions for the 3' untranslated region of eukaryotic transcripts. Curr. Opin. Cell Biol. 7:386-392.   DOI   ScienceOn
7 Han SH, Cho IC, Kim JH, Ko MS, Kim YH, Kim EY, Park SP and Lee SS. 2011. Coat color patterns and genotypes of extension and agouti in Hanwoo and Jeju Black cattle. J. Life Sci. 494-501.
8 Klungland H, Vage DI, Gomez-Raya L, Adalsteinsson S and Lien S. 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm. Genome 6:636-639.   DOI   ScienceOn
9 Olson TA. 1999. Genetics of color variation. In: Fries R and Ruvinsky A (Eds.), The Genetics of Cattle. Wallingford, UK, CABI, pp. 33-53.
10 Reinsch N, Thomsen H, Xu N, Brink M, Looft C, Kalm E, Brockmann GA, Grupe S, Kühn C, Schwerin M, Leyhe B, Hiendleder S, Erhardt G, Medjuqorac, I, Russ I, Forster M, Reents R and Averdunk G. 1999. A QTL for the degree of spotting in cattle shows synteny with the KIT locus on chromosome 6. J. Hered. 90(6):629-634.   DOI
11 Rouzaud F, Annereau JP, Valencia JC, Costin GE and Hearing VJ. 2003. Regulation of melanocortin 1 receptor expression at the mRNA and protein levels by its natural agonist and antagonist. FASEB J. 17: 2154-2156.   DOI
12 Rouzaud F, Martin J, Gallet PF, Delourme D, Goulemot-Leger V, Amigues Y, Menissier F, Leveziel H, Julien R and Oulmouden A. 2000. A first genotyping assay of French cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (MC1R). Genet. Sel. Evol. 32 (5):511-520.   DOI   ScienceOn
13 Rouzaud F, Oulmouden A and Kos L. 2010. The untranslated side of hair and skin mammalian pigmentation: Beyond coding sequences. IUBMB Life 62(5): 340-346.
14 Schmutz SM, Berryere TG, Ciobanu DC, Mileham AJ, Schmidtz BH and Fredholm M. 2004. A form of albinism in cattle is caused by a tyrosinase frame shift mutation. Mamm. Genome 15(1):62-67.   DOI   ScienceOn
15 김태헌, 윤두학, 박응우, 이혜영, 오성종, 정일정, 탁태영, 김경남, 한재용. 2000. 소 품종별 Melanocortin Receptor 1(MC1R) 유전자의 유전자형 빈도에 관한 연구. J. Anim. Sci. & Technol. 42(6):735-744.
16 Schmutz SM and Dreger DL. 2013. Interaction of MC1R and PMEL alleles on solid coat colors in Highland cattle. Anim. Genet. 44(1):9-13.   DOI   ScienceOn
17 Seitz JJ, Schmutz SM, Thue TD and Buchanan FC. 1999. A missense mutation in the bovine MGF gene is associated with the roan phenotype in Belgian Blue and Shorthorn cattle. Mamm. Genome 10(7):710-712.   DOI   ScienceOn
18 Seo KS, Mohanty TR, Choi T and Hwang IH. 2007. Biology of epidermal and hair pigmentation in cattle: a mini-review. Vet. Dermatol. 18:392-400.   DOI   ScienceOn
19 도경탁, 신희영, 이종혁, 김내수, 박응우, 윤두학, 김관석. 2007. 한우에서 모색 관련 유전자 변이에 관한 연구. J. Anim. Sci. & Technol. 49(6):711-718.   DOI
20 박재희, 이창우, 이해이, 최재원, 최연호, 권아남, 지연희, 김종국. 2014. 칡소 씨수소의 MC1R 유전자형과 자손의 모색 발현. J. Emb. Trans. 29(1):21-27.   DOI
21 박재희, 이해이, 김용수, 김종국. 2012. MC1R 유전자형의 유전자형과 칡소의 모색 발현 및 비경색 분포에 관한 연구. J. Anim. Sci. & Technol. 54(4):255-265.   DOI   ScienceOn
22 이성수, 양보석, 양영훈, 강승률 고서봉, 정친관, 오운용, 오성종, 김규일. 2002. 칡소와 비경 흑색 한우의 Melanocortin Receptor 1(MC1R) 유전자형 분석 J. Anim. Sci. & Technol. 44(1):23-30.   DOI   ScienceOn
23 정의룡, 김우태, 김연수, 한상기. 2000. 소 모색관련 유전자 MC1R의 PCR-RFLP Marker를 이용한 한우육 판별. J. Anim. Sci. & Technol. 42(4):379-390.
24 진실, 심정미, 서동원, 정우영, 류승희, 김진호, 이준헌. 2011. 한우 후보씨수소 및 칡소와 흑소에서 MC1R 유전자의 유전자형 분석. CNU J. Agri. Sci. 38(3):453-458.
25 한국종축개량협회. 2008. 한우기준공고(공고 2008-2호).