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The Effect of Cryopreservation Condition on Developmental Rate of Pronuclear Stage Embryos and Vitrification of Mouse Oocytes  

Kim, Ji-Chul (Department of Animal Resources, Daegu University)
Park, Sung-Baek (Department of Animal Resources, Daegu University)
Nam, Yoon-Sung (ROSA Infertility Clinic)
Seo, Byoung-Boo (Department of Animal Resources, Daegu University)
Kim, Jae-Myeoung (Medical Center of CHA Gerneral Hospital)
Song, Hai-Bum (Department of Animal Resources, Daegu University)
Publication Information
Journal of Embryo Transfer / v.26, no.3, 2011 , pp. 201-207 More about this Journal
Abstract
The present study was performed to investigate the survival and subsequent embryonic developmental rate of immature and mature oocytes after vitrification and pronuclear stage embryos after slow-freezing and vitrification. We have also tried to examine the dependency of concentrations (7.5, 15%) and exposure time (5, 10, 20 min) of ED cryoprotectant on developmental rate of pronuclear stage embryos. The developmental rates of 2-ce1l and blastocyst embryos at mature oocytes were significantly (p<0.05) higher than immature oocytes. After slow freezing, vitrification and thawing of pronuclear stage embryo, the survival and developmental rates of blastocysts and hatched blastocysts were significantly (p<0.05) higher after vitrification than after slow-freezing. On contrary, the developmental rates of 2-cell embryos were significantly (p<0.05) higher after slow freezing than after vitrification. The cryopreservation methods of pronuclear stage embryos vitrified by exposed to 7.5% ED solution for 5 minutes was significantly (p<0.05) higher than other experimental group. The results of our study suggest 1hat the developmental rates of mature oocytes have been more successful than immature oocytes during vitrification. Vitrification was more efficient than slow freezing in case of pronuclear stage embryos. The effective cryopreservation method of pronuclear stage embryos was vitrified by exposed to 7.5% ED solution for 5 minutes.
Keywords
slow-freezing; vitrification; cryoprotectant; oocyte; pronuclear stage embryo;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Papis K, Shimizu M and Izaike Y. 1999. The effect of gentle pre-equilibration on survival and development rates of bovine in vitro matured oocytes vitrified in droplets. Theriogenology 51:173.   DOI   ScienceOn
2 Park MC, Kim JY, Kim SB, Park YS, Park HD and Lee JH, et al. 2009. The effect of cryopreservation on the mouse embryos at various-pronuclear stages. Asian-Aust. J. Anim. Sci. 22:174-180.   DOI
3 Saha S, Otoi T, Takagi M, Boediono A, Sumantri C and Suzuki T. 1996. Normal calves obtained after direct transfer of vitrified bovine embryos using ethylene glycol, trehalose, and polyvinylpyrrolidone. Cryobiology a;33:291-299.   DOI   ScienceOn
4 Kasai M, Iritani A and Chang MC. 1979. Fertilization in vitro of rat ovarian oocytes after freezing and thawing. Biol. Reprod. 21:839-844.   DOI   ScienceOn
5 Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T and Machida T. 1990. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fertil. 89:91-97.   DOI
6 Kim EK, Kim MY, Son SM and Kim DW. 2008. Comparison of the efficiency between slow freezing and vitrification method for cryopreservation of human embryos. J. Emb. Trans. 23(1):19-24.
7 Kim MY and Lee YL. 2007. Comparison of vitrification and slow freezing for the cryopreservation of mouse pronuclear stage embryos. Kor. J. Reprod. Mrd. 34(2):117-123.
8 Kuleshova LL, Shaw JM and Trounson AO. 2001. Studies on replacing most of the penetrating cryoprotectant by polymers for embryo cryopreservation. Cryobiology 43:21-31.   DOI   ScienceOn
9 Landa V and Tepla O. 1990. Cryopreservation of mouse 8-cell embryos in microdrops. Folia. Biol. 36:153-158.
10 Leibo SP and Oda K. 1993. High survival of mouse zygotes and embryos cooled rapidly or slowly in ethylglycol plus polyvinypyrrolidone. Cryo-Letters 14:133-144.
11 Li R, Lai L, Wax D, Hao Y, Murphy CN and Rieke A, et al. 2006. Cloned transgenic swine via in vitro production and cryopreservation. Biol. Reprod. 75:226-230.   DOI   ScienceOn
12 Friedler S, Giudice LC and Lamb EJ. 1988. Cryopreservation of embryos and ova. Fertil. Steril. 49:743-764.   DOI
13 Hamlett DK, Franken DR, Cronje HS and Luus H. 1989. Murine oocyte cryopreservation: Comparison between fertilization success rates of fresh and frozen metaphase I and II oocytes. Arch. Andol. 23:27.   DOI
14 Arav A, Bacci ML and Rubinsk B. 1990. Vitrification of immature pig oocytes. In Preceding of the 11th International Pig Veterinar Society Congress Lausanne Abst. 479.
15 Herrler A, Rath D and Nieman H. 1991. Effect of cryoprotectent on fertilization and cleavage of bovine oocytes in vitro. Theriogenology 35:212(Abst.).   DOI
16 Hochi S, Akira K, Ken K and Akira H. 1997. In vitro fertilization ability of bovine oocyte frozen-thawed at immature, maturing and maturestage. J. Mamm. Ova. Res. 14:61-65.   DOI   ScienceOn
17 Isachenko V, Montag M, Isachenko E, Nawroth F, Dessole S and van der Ven H. 2004. Developmental rate and ultrastructure of vitrified human pronuclear oocyte after step-wise versus direct rehydration. Hum. Reprod. 19:660-665.   DOI
18 Bautista JA, Dela Pena EC, Katagiri S, Takahashi Y and Kanagawa H. 1998. In vitro viability of mouse oocytes vitrified in an ethyleneglycol-based solution. Jpn. J. Vet. Res. 46:13-18.
19 Bos-Mikich A, Wood MJ, Candy CJ and Whittingham DG. 1995. Cryogenetical analysis and developmental potential of vitrified mouse oocytes. Biol. Reprod. 53:780-785.   DOI   ScienceOn
20 Chen C. 1986. Pregnancy after human oocyte cryopreservation. Lancet. I. 884-886.
21 Dobrinsky JR, Pursel VG, Long CR and Johnson LA. 2000. Brith of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification. Biol. Reprod. 62: 564-570.   DOI   ScienceOn
22 Chen SU, Lien YR, Chen HF, Chao KH, Ho HN and Yang YS. 2000. Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws. Hum. Reprod. 15:2598-2603.   DOI
23 Cohen J, DeVane GW, Elsner CW, Fehilly CB, Kort HI and Massey JB. 1988. Cryopreservation of zygotes and early cleaved human embryos. Fertil. Steril. 49:283-289.   DOI
24 Cong PQ, Song ES, Kim ES, Li ZH, Zhang YH and Lee JM. 2007. Effect of cryoprotectant, warming soultion and removal of lipid on viability of porcine nuclear transfer embryos vitrified by open pulled straw method. Reprod. Dev. Biol. 31(2):103-108.
25 Dochi O, Oshima K, Takenouchi N and Komatsu M. 1998. Effect of exposure to cryoprotectent solutions in cleavage and subsequent development of immature bovine oocyte in vitro. Theriogenology Abst. 49(1):167.   DOI   ScienceOn
26 Fahning ML and Garcia MA. 1992. Status of cryopreservation of embryos from domestic animals. Cryobiology 29:1-18.   DOI   ScienceOn
27 Fahy GM, MacFarlane DR, Angell CA and Meryman HT. 1987. Vitrification as an approach to cryopreservation. Cryobiology 24:387-402.   DOI   ScienceOn
28 Tsunoda Y, Parkening TA and Chang MC. 1976. In vitro fertilization of mouse and hamster egg after freezing and thawing. Experimentia 32:223-224.   DOI   ScienceOn
29 Al-Hasani S, Kirsch J, Diedrich K, Blanke S, Van der Ven H and Krebs D. 1989. Successful embryo transfer of cryopreserved and in vitro fertilized rabbit oocytes. Hum. Reprod. 4:77-79.   DOI
30 Takagi M, Boediono A, Saha S and Suzuki T. 1993. Survival rate of frozen-thawed bovine IVF embryos in relation to exposure time using various cryoprotectants. Cryobiology 30:306-312.   DOI   ScienceOn
31 Vajta G, Holm P, Kuwayama M, Booth Pj, Jacobsen H and Greve T, et al. 1998. Open pulled straw (OPS) vitrification : A new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Develop. 51:53-58.   DOI   ScienceOn
32 Van der Elst J, Van den Abbeel E, Jacobs R, Wisse E and Van Sterirtegheem A. 1988. Effect of 1,2-propanediol and dimethylsulphoxide on the meiotic spindle of the mouse oocyte. Hum. Reprod. 3:960-967.   DOI
33 Vincent C, Pickering SJ and Johnson MH. 1990. The hardening effect of dimethylsulphoxide on the mouse zona pellucida requires the presence of an oocyte and is associated with a reduction in the number of cortical granules present. J. Reprod. Fertil. 89:253-259.   DOI
34 Moller CC and Wassarman PM. 1989. Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs. Dev. Biol. 132:103-112.   DOI   ScienceOn
35 Mandelbaum J, Junka AM, Plachot M and Alnot MD. 1987. Cryopreservation of human embryos and oocytes. Hum. Reprod. 3:117.
36 Martino A, Songsasen N and Leibo SP. 1996. Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Bio. Reprod. 54:1059-1069.   DOI   ScienceOn