Browse > Article

Fibrinogen mRNA Expression Up-Regulated in Follicular Cyst of Korean Cattle  

Tak, Hyun-Min (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine)
Han, Jae-Hee (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine)
Kang, Da-Won (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine)
Publication Information
Journal of Embryo Transfer / v.25, no.1, 2010 , pp. 29-34 More about this Journal
Abstract
Follicular cystic ovary (FCO) is one of the major causes of reproductive failure in cattle. Genetic alterations affect the function of diverse cells and/or tissues, which could be present in cystic ovaries. A microarray analysis was performed to screen differential gene expressions in follicular cystic follicles of cattle. In this study, we hypothesized that follicular cysts may be induced by changes in ion- and transporter-related gene expression. Microarray data showed that fibrinogen-gamma (FGG) and low density lipoprotein receptor-related protein 8 (LRP8) were up-regulated, while choline transporter-like protein 4 (SLC44A4), very long-chain acyl-CoA synthetase homolog 2 (SLC27A5), annexin 8 (ANXA8), and aquaporin 4 were down-regulated in follicular cystic follicles. A semi-quantitative RT-PCR was carried out to validate DEGs altered in follicular cystic follicles. Of six DEGs, three DEGs (FGG, SLC44A4, and aquaporin 4) showed a positive correlation between microarray and semi-quantitative PCR data. We focused on FGG, among three DEGs, which was highly up-regulated in follicular cystic follicles. The FGG mRNA was upregulated by 8.4-fold and by 1.7-fold in the bovine follicular cystic follicles as judged by microarray and RT-PCR analysis, respectively. However, there was no significant changes in the expression level of FGG protein in both follicular cystic follicles and granulosa cells isolated from follicular cystic follicles by Western blot analysis. Although this study does not reveal a positive correlation between the mRNA and protein level, FGG appears to be an important biomarker in the discrimination of follicular cyst from normal ovary.
Keywords
Korean cattle; microarray; follicular cyst; fibrinogen;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Whiteley W, Jackson C, Lows G, Rumley A, Sandercock P, Wardlaw J, Dennis M and Sudlow C. 2009. Inflammatory markers and poor outcome after stroke: A prospective cohort study and systematic review of interleukin-6. PLoS. Med. 6:e10000145.
2 Shalgi R, Kraicer P, Rimon A, Pinto M and Soferman N. 1973. Proteins of human follicular fluid: The blood-follicle barrier. Fertil. Steril. 24:429-434.
3 Shimada H, Kasakura S, Shiotani M, Nakamura K, Ikeuchi M, Hoshino T, Komatsu T, Ihara Y, Sohma M, Maeda Y, Matsuura R, Nakamura S, Hine C, Ohkura N, and Kato H. 2001. Hypocoagulable state of human preovulatory ovarian follicular fluid: Role of sulfated proteoglycan and tissue factor pathway inhibitor in the fluid. BioI. Reprod. 64: 1739- 1745.   DOI   ScienceOn
4 Lingenfelter BM, Dailey RA, Inskeep EK, Vernon MW, Poole DH, Rhinehart JD and Yao J. 2008. Microarray analysis of gene expression in granulosal cells from persistent follicles in cattle. Anim. Reprod. Sci. 104:405-413.   DOI   ScienceOn
5 Lucy MC, Savio JD, Badinga L, De La Sota RL and Thatcher WW. 1992. Factors that affect ovarian follicular dynamics in cattle. J. Anim. Sci. 70:3615-3626.
6 Osterberg L, Akeson M, Levan K, Partheen K, Zetterqvist BM, Brannstrom M and Horvath G. 2006. Genetic alterations of serous borderline tumours of the ovary compared to stage I serous ovarian carcinomas. Cancer Genet. Cytogenet. 167: 103-108.   DOI   ScienceOn
7 Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, Celestin J, Leder P and Luster AD. 1996. Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat. Med. 2:449-456.   DOI   ScienceOn
8 Parrott JA, Whaley PD and SkinnerNK. 1993. Extrahepatic expression of fibrinogen by granulosa cells: Potential role in ovulation. Endocrinology 133:1645-1649.   DOI   ScienceOn
9 Peter AT. 2004. An update on cystic ovarian degeneration in cattle. Reprod. Domest. Anim. 39: 1-7.   DOI   ScienceOn
10 Choe CY, Son DS, Choi GC, Song SH, Choe CY, Choi SH, Kim HJ, Cho SR, Hur CG and Kang DW. 2006. Survey on the incidence of reproductive disorders in Hanwoo. J. Emb. Trans. 21:331-338.
11 Choe CY, Park SY, Kim ES, Moon YJ, Park HJ, Son DS, Cho SR, Kim HJ, Kim JB, Park N, Hong SG, Han JH and Kang DW. 2008. Identification of inflammation-related genes altered in the cystic ovary and endometritis of Korean cattle. J. Emb. Trans. 23:211-216.
12 Hamilton SA, Garverick HA, Keisler DH, Xu ZZ, Loos K, Youngquist RS and Salfen BE. 1995. Characterization of ovarian follicular cysts and associated endocrine profiles in dairy cows. BioI. Reprod. 53:890-898.   DOI   ScienceOn
13 Hauptmann S, Denkert C, Koch I, Petersen S, Schluns K, Reles A, Dietel M and Petersen I. 2002. Genetic alterations in epithelial ovarian tumors analyzed by comparative genomic hybridization. Hum. Pathol. 33:632-641.   DOI   ScienceOn
14 Iwaki T and Castellino FJ. 2005. Maternal fibrinogen is necessary for embryonic development. Curr Drug Targets 6:535-539.   DOI   ScienceOn
15 Chae IS, Jang DM, Cheong HT, Yang BK and Park CK. 2009. Changing proteins in granulosa cells during follicular development in pig. Reprod. Dev. BioI. 33:183-187.