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Growth Characteristics and Variation of Reproductive Physiology in SCNT Cloned Male Hanwoo Calves  

Bae, Seong-Hun (Biotechnology Institute, Seoulmilk)
Yang, Byoung-Chul (Animal Biotechnology Division, National Institute of Animal Science)
Ko, Yeoung-Gyu (Animal Biotechnology Division, National Institute of Animal Science)
Oh, Keon-Bong (Animal Biotechnology Division, National Institute of Animal Science)
Seong, Hwan-Hoo (Animal Genetic Improvement Division, National Institute of Animal Science)
Min, Kwan-Sik (Hankyong National University)
Park, Eung-Woo (Animal Biotechnology Division, National Institute of Animal Science)
Park, Soo-Bong (Animal Biotechnology Division, National Institute of Animal Science)
Hwang, Seong-Soo (Animal Biotechnology Division, National Institute of Animal Science)
Publication Information
Journal of Embryo Transfer / v.24, no.3, 2009 , pp. 177-182 More about this Journal
Abstract
This study was conducted to investigate the variation of growth characteristics and reproductive physiology in cloned Hanwoo male calves during growing stage. The hematological parameters, body weight, and plasma hormonal levels, birth to 12 months, were analyzed in the cloned calves (n=3). Differences among treatment means were determined by a student t-test. A probability of P<0.05 was considered statistically significant. The hematological parameters, such as white blood cell, red blood cell, and platelet, were not different in both normal and cloned calves. The difference of body weight, however, was significantly higher in the cloned calves, $5{\sim}6$ months (p<0.05) and $7{\sim}12$ months (p<0.01), than that of the comparators, respectively. The plasma IGF-1 level was statistically significant in the cloned calves, $5{\sim}10$ months, compared to that of the normal calves (p<0.05). However, the plasma testosterone level was not different in both normal and clone calves according to growing stage. Taken together, the cloned Hanwoo male calves are growing faster and maintaining a normal reproductive physiology.
Keywords
cloned Hanwoo male calves; hematological parameters; body weight; IGF-1; testosterone;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Brito LFC, Barth AD, Rawlings NC, Wilde RE, Crews Jr DH, Mir PS and Kastelic JP. 2007b. Effect of nutrition during calfhood and peripubertal period on serum metabolic hormones, gonadotropins and testosterone concentrations, and on sexual development in bulls. Domestic Animal Endocrinology 33:1-18   DOI   ScienceOn
2 Kasai K, Sano F, Miyashita N, Watanabe S and Nagai T. 2007. Comparison of the growth performances of offspring produced by a pair of cloned cattle and their nuclear donor animals. J. Reprod. Dev. 53:135-142   DOI   ScienceOn
3 Lanza RP, Cibelli JB, Faber D, Sweeney RW, Henderson B, Nevala W, West MD and Wettstein PJ. 2001. Cloned cattle can be healthy and normal. Science 294:1893-1894   DOI   PUBMED   ScienceOn
4 Zawada WM, Cibelli JB, Choi PK, Clarkson ED, Golueke PJ, Witta SE, Bell KP, Kane J, Ponce de Leon FA, Jerry DJ, Robl JM, Freed CR and Stice SL. 1998. Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats. Nat. Med. 4:569-574   DOI   ScienceOn
5 이성수. 1998. 거세 한우의 혈청성분과 경제형질의 상관관계에 관한 연구. 서울대학교 박사학위논문
6 홍성구, 성환후, 고응규, 박찬국, 신기준, 장원경, 박용윤. 1998. 거세한우의 성장 단계별 혈중 Testosterone, Cortisol 및 In-sulin-like growth factor-1(IGF-1) 농도의 변화. 축산기술연구보고서 312-460
7 황성수, 양병철, 임기순, 고응규, 최선호, 민관식, 윤종택, 성환후. 2008c. hCG 투여가 복제란 이식 한우 대리모의 임신과 progesterone 농도에 미치는 영향. 한국수정란이식학회지 23:177-181
8 Caprio M, Fabbrini E, Ricci G, Basciani S, Gnessi L, Arizzi M, Carta A, Martino M, Isidori A and Frajese G. 2003. Ontogenesis of leptin receptor in rat Leydig cells. BioI. Reprod. 68:1199-1207   DOI
9 배성훈, 황성수, 양병철, 고응규, 김동훈, 임기순, 최화식, 진동일, 양보석, 성환후. 2007. 체세포 복제 한우 수소의 정액 성상, 정자의 활동성 및 수정 능력 분석. 한국동물번식학회지 31:139-143
10 Hill JR, Roussel AJ, Cibelli JB, Edwards JF, Hooper NL, Miller MW, Thompson JA, Looney CR, Westhusin ME, Robl JM and Stice SL. 1999. Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies). Theriogenology 51:1451-1465   DOI   ScienceOn
11 황성수, 최선호, 장유민, 고응규, 양병철, 임기순, 민관식, 성환후. 2008b. 체세포 복제란 이식 한우의 분만 전 혈장 Pro-gesterone과 Estradial-17 $\beta$ 농도 변화. 한국동물번식학회지 32:199-203
12 Dean W, Santos F, Stojkovic M, Zakhartchenko V, Walter J, Wolf E and Reik W. 2001. Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. Proc. Natl. Acad. Sci. USA 98:13734-13738   DOI   ScienceOn
13 Hill JR, Burghardt RC, Jones K, Long CR, Looney CR, Shin T, Spencer TE, Thompson JA, Winger QA and Westhusin ME. 2000. Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses. BioI. Reprod. 63:1787-1794   DOI   ScienceOn
14 Wells DN, Forsyth JT, McMillan V and Oback B. 2004. The health of somatic cell cloned cattle and their offspring. Cloning Stem Cells 6:101-110   DOI   ScienceOn
15 Yang BC, Im GS, Kim DH, Yang BS, Oh HJ, Park HS, Seong HH, Kim SW, Ka HH and Lee CK. 2008. Development of vitrified-thawed bovine oocytes after in vitro fertilization and somatic cell nuclear transfer. Anim. Reprod. Sci. 103:25-37   DOI   PUBMED   ScienceOn
16 황성수, 장유민, 고응규, 양병철, 임기순, 김명직, 민관식, 윤종택, 김창근, 성환후. 2008a. 체세포 복제란 이식우의 분만 전.후 TGF-$\beta_{1}$ 단백질 농도. 한국동물번식학회지 32:27-31
17 Faber DC, Ferre LB, Metzger J, Robl JM and Kasinathan P. 2004. Agro-economic impact of cattle cloning. Cloning Stem Cells 6:198-207   DOI   ScienceOn
18 Wang GM, O'Shaughnessy PJ, Chubb C, Robaire B and Hardy MP. 2003. Effects of insulin-like growth factor I on steroidogenic enzyme expression levels in mouse Leydig cells. Endocrinology 144:5058-5064   DOI   ScienceOn
19 Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KH. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813   DOI   ScienceOn
20 Yonai M, Kaneyama K, Miyashita N, Kobayashi S, Goto Y, Bettpu T and Nagai T. 2005. Growth, reproduction, and lactation in somatic cell cloned cows with short telomeres. J. Dairy Sci. 88:4097-4110   DOI   ScienceOn
21 권응기, 김현섭, 남기택, 윤상기, 김종복, 홍병주. 2001. Holstein 수소와 거세우 및 Zeranol 투여 거세우의 성장 단계별 혈액상과 혈청 대사물질 및 호르몬 농도의 변화. 동물자원과학회지 43:503-512
22 McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE and Kind AJ. 2001. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405:1066-1069   DOI   ScienceOn
23 Lin T. 1995. Regulation of Leydig cell function by insulin-like growth factor-I and binding proteins. J. Androl. 16:193-196   PUBMED
24 권응기, 김현섭, 윤상기, 남기택, 김종복, 안병석, 김준식. 2000. rbST 투여가 Holstein 비육우의 혈중 및 도체 호르몬 농도변화에 미치는 영향. 동물자원과학회지 42:451-458
25 Brito LFC, Barth A, Rawlings N, Wilde R, Crews D Jr, Mir P and Kastelic J. 2007a. Circulating metabolic hormones during the peripubertal period and their association with testicular development in bulls. Reprod. Domest. Anim. 42:502-508   DOI   ScienceOn
26 Bruckrnair RM, Leharm E, Hugi D, Hammon HM and Blum JW. 1998. Ultrasonic measurement of longissimus dorsi muscle and backfat, associated with metabolic and endocrine traits, during fattening of intact and castrated male cattle. Livestock Production Science 53:123-134   DOI   ScienceOn