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Comparisons of Estrus Induction with Anti-Prolactin and Hormones in Bitches  

Heo, Young (College of Veterinary Medicine, Gyeongsang National University)
Kang, Eun-Ju (College of Veterinary Medicine, Gyeongsang National University)
Maeng, Geun-Ho (College of Veterinary Medicine, Gyeongsang National University)
Kim, Min-Jung (College of Veterinary Medicine, Gyeongsang National University)
Jo, Gyu-Wan (College of Veterinary Medicine, Gyeongsang National University)
Lee, Sung-Lim (College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Embryo Transfer / v.24, no.3, 2009 , pp. 169-176 More about this Journal
Abstract
Domestic bitches are non-seasonally monoestrus; spontaneously ovulate only once or twice occurs at anytime of the year. Estrus induction has been applied infrequent estrus, misleading ovulation, mating difficulties, failure to conceive after normal mating, pregnancy failure and biological research. Protocol of estrus induction which included variable hormones such as FSH, GnRH, and PMSG have been applied for the last decades. Recently, Bromocriptine, one of anti-prolatin/dopamine agonist has been occasionally applied for estrus induction. The study was carried out to investigate the effective method for the induction of estrus in bitches using different hormone treatments, and the initiation time of estrus from hormone treatment by assessments of cytological observation and blood plasma progesterone concentration. A total of 54 bitches on anestrus were selected for the study and divided randomly into 8 treatment groups as follow. Control, natural estrus; FSH (L), FSH (1.5 mg/kg, twice a day, $Falltrophin^{(R)}$, Vetrepharm); FSH (H), FSH (3.0 mg/kg, twice a day); GnRH+FSH, GnRH (5 ug/kg, once first day, $GNADON^{(R)}$, Dongbang)+FSH (3 mg/kg, SID); PMSG, PMSG (50 IU/kg, every third day $FOLLIGON^{(R)}$, Intevet); GnRH+PMSG, GnRH (5 ug/kg, only first day)+PMSG (50 IU/kg, every third day); GnRH, GnRH (5 ug/kg, only first day); Bromocriptine, bromocriptine (0.3 mg/kg, SID, $Parlodel^{(R)}$, Novartis). The bitches were evaluated clinical sign, cytological exam and $OVUCHECK^{(R)}$ Premate for assessment of estrus induction. Estrus induction rates were significantly (P<0.05) higher in GnRH+PMSG (100%) compared to others. PMSG and GnRH+PMGS (87.5 and 100%) and Bromocriptine (77.8%) were higher than others except GnRH+PMSG. Analysis of vaginal smear has proved to be effective a correct assessment of estrus induction with assay of progesterone concentration by $OVUCHECK^{(R)}$ Premate. Proestrus initiated by the $6^{th}$ after induction in most case. In conclusion, bromocriptine is an effective drug for estrus induction in bitches and assay of progesterone concentration by $OVUCHECK^{(R)}$ Premate with examination of vaginal smear that should be useful to detection of estrus induction of estrus induced bitches.
Keywords
bitch; anti-prolactin; hormones; estrus induction;
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1 England GC and Allen WF. 1991. Repeatability of events during spontaneous and gonadotrophins-induced oestrus in bitches. J. Reprod. Fertil. 93:443-448   DOI   PUBMED   ScienceOn
2 Grundy SA, Feldman E and Davidson A. 2002. Evaluation of infertility in the bitch. Clin. Tech. Small. Anim. Pract. 17:108-115   DOI   ScienceOn
3 Jeffcoate IA and Lindsay FE. 1989. Ovulation detection and timing of insemination based on hormone concentrations, vaginal cytology and the endoscopic appearance of the vagina in domestic bitches. J. Reprod. Fertil. Suppl. 39:277-287   PUBMED
4 Post K. 1985. Canine vaginal cytology during the estrus cycle. Can. Vet. J. 26:101-104   PUBMED
5 Renton JP, Munro CD, Heathcote RH and Carmichael S. 1981. Some aspects of the aetiology, diagnosis and treatment of infertility in the bitch. J. Reprod. Fertil. 61:289-294   DOI   PUBMED   ScienceOn
6 Wanke MM, Loza ME and Rebuelto M. 2006. Progestin treatment for infertility in bitches with short interestrus interval. Theriogenology 66:1579-1582   DOI   ScienceOn
7 Weilenmann R, Arnold S, Dobeli M, Rusch P and Zerobin K. 1993. Estrus induction in bitches by the administration of PMSG and HCG. Schweiz. Arch. Tierheilkd. 135:236-241   PUBMED
8 Nakao T, Aoto Y, Fukushima S, Moriyoshi M and Kawata K. 1985. Induction of estrus in bitches with exogenous gonadotropins, and pregnancy rate and blood progesterone profiles. Nippon Juigaku. Zasshi. 47:17-24   DOI   PUBMED   ScienceOn
9 Bouchard G, Youngquist RS, Vaillancourt D, Krause GF, Guay P and Paradis M. 1991b. Seasonality and variability of the interestrous interval in the bitch. Theriogenology 36:41-50   DOI   ScienceOn
10 Bouchard G, Youngquist RS, Clark B, Concannon PW and Braun WF. 1991a. Estrus induction in the bitch using a combination diethylstilbestrol and FSH-P. Theriogenology 36:51-65   DOI   ScienceOn
11 Johnston SD, Olson PN and Root MV. 1994. Clinical approach to infertility in the bitch. Seminars in Veterinary Medicine and Surgery (Small Animal) 9:2-6   PUBMED
12 Mizokawa T, Akai T, Nakada Y, Yamaguchi M, Nakagawa H, Hasan S, Rettig KJ and Wachtel H. 1993. Terguride as a new anti-hyperprolactinemic agent: characterization in rats and dogs in comparison with bromocriptine. Jpn. J. Pharmacol. 63:269-278   DOI   PUBMED
13 Mc Rae GI, Roberts BB, Worden AC, Bajka A and Vickery BH. 1985. Long term reversible suppression of estrus in bitches with nafarelin acetate, a potent LHRH agonist. J. Reprod. Fertil. 74:389-397   DOI   PUBMED   ScienceOn
14 Thun R, Watson P and Jackson GL. 1977. Induction of estrus and ovulation in the bitch, using exogenous gonadotropins. Am. J. Vet. Res. 38:483-486   PUBMED
15 Beijerink NJ, Dieleman SJ, Kooistra HS and Okkens AC. 2003. Low doses of bromocriptine shorten the interestrous interval in the bitch without lowering plasma prolactin concentration. Theriogenology 60: 1379-1386   DOI   ScienceOn
16 Gobello C. 2006. Dopamine agonists, anti-progestins, anti-androgens, long-term-release GnRH agonists and anti-estrogens in canine reproduction: a review. Theriogenology 66:1560-1567   DOI   ScienceOn
17 Allen NE and Stockman A. 1979. Pseudopregnancy in bitch. Vet. Record. 104:22   DOI
18 Jones GE, Boyns AR, Bel ET, Christie DW and Parkes MF. 1973. Immunoreactive luteinizing hormones and progesterone during pregnancy and following gonadotrophin administration in beagle bitches. Acta. Endocrinol. 72:573-581   DOI   PUBMED
19 Arnold S, Arnold P, Concannon PW, Weilenmann R, Hubler M, Casal M, Dobeli Fairburn A, Eggenberger E and Rusch P. 1989. Effect of duration of PMSG treatment on induction of oestrus, pregnancy rates and the complications of hyper-oestrogenism in dogs. J. Reprod. Fertil. Suppl. 39:115-122   PUBMED
20 Van der Holst Wand Best AP. 1976. Een beschouwing over het meest geschikre tijdstip voor de dekking van de teef. Tijdschr. Diergeneesk. 19:125-133
21 Jeffcoate IA and England GC. 1997. Urinary LH, plasma LH and progesterone and their clinical correlates in the periovulatory period of domestic bitches. J. Reprod. Fertil. 51:267-275
22 Okkens AC, Kooistra HS, Dieleman SJ and Bevers MM. 1997. Dopamine agonistic effects as opposed to prolactin concentrations in plasma as the influencing factor on the duration of anestrus in bitches. J. Reprod. Fertil. 51:55-58
23 Van Haaften B, Dieleman SJ, Okkens AC, Bevers MMand Willemse AH. 1989. Induction of estrus and ovulation in dogs by treatment with PMSG and/or bromocriptine. J. Reprod. Fertil. 39:330-331
24 Kutzler MA. 2005. Induction and synchronization of estrus in dogs. Theriogenology 64:766-775   DOI   PUBMED   ScienceOn
25 Paisley LG and Fahning ML. 1977. Effects of exogenous follicle-stimulating hormone and luteinizing hormone in bitches. J. Am. Vet. Med. Assoc. 171:181-185   PUBMED
26 Zoldag L, Fekete S, Csaky I and Bersenyi A. 2001. Fertile estrus induced in bitches by bromocriptine, a dopamine agonist: a clinical trial. Theriogenology 55:1657-1666   DOI   ScienceOn
27 Inaba T, Umerhara T, Mori J, Torri R, Tamada H and Sawada T. 1996. Reversible suppression of pituitary testicular function by a sustained release formulation of GnRH agonist (leuprolide acetate) in dogs. Theriogenology 46:671-677   DOI   ScienceOn
28 Christie DW and Bell ET. 1972. Studies on canine reproductive behaviour during the normal oestrous cycle. Anim. Behav. 20:621-631   DOI   ScienceOn
29 Gobello C, Castex G, Corrada Y, Klima L, de la Sota RL and Rodriguez R. 2002. Use of prostaglandins and bromocriptine mesylate for pregnancy termination in bitches. J. Am. Vet. Med. Assoc. 220:1017-1019   DOI   ScienceOn
30 Fay J, Mezo T, Solti L, Wolfling A and Abonyi-Toth Z. 2003. Comparison of different methods used for oestrus examination in the bitch. Acta. Vet. Hung. 51:385-394   DOI   ScienceOn
31 Shille VM, Thatcher MJ and Simmons KJ. 1984. Efforts to induce estrus in the bitch, using pituitary gonadotropins. J. Am. Vet. Med. Assoc. 184:1469-1473   PUBMED
32 Gobello C, de la Sota RL and Goya RG. 2001. Study of the change of prolactin and progesterone during dopaminergic agonist treatments in pseudopregnant bitches. Anim. Reprod. Sci. 66:257-267   DOI   ScienceOn
33 Bell ET, Bailey JB and Christie DW. 1973. Studies on vaginal cytology during the canine oestrous cycle. Res. Vet. Sci. 14: 173-179   PUBMED
34 Okkens AC and Kooistra HS. 2006. Anoestrus in the dog: a fascinating story. Reprod. Domest. Anim. 41:291-296   DOI   ScienceOn
35 Kutzler MA. 2007. Estrus induction and synchronization III canids and felids. Theriogenology 68:354-374   DOI   PUBMED   ScienceOn
36 Concannon PW, Temple M, Montanez A and Newton L. 2006. Effects of dose and duration of continuous GnRH-agonist treatment on induction of estrus in beagle dogs: competing and concurrent up-regulation and down-regulation of LH release. Theriogenology 66:1488-1496   DOI   ScienceOn
37 Janssens LA. 1986. Treatment of pseudopregnancy with bromocriptin, an ergot alkaloid. Vet. Rec. 119:172-174   DOI   PUBMED   ScienceOn