Browse > Article

Apoptosis-related Genes Altered in Bovine Cystic Ovary  

Tak, Hyun-Min (Department of Physiology, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
Kim, Gyu-Tae (Department of Physiology, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
Kim, Eun-Jin (Department of Physiology, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
Mun, Yun-Ja (Department of Physiology, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science RDA)
Son, Dong-Soo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Han, Jae-Hee (Department of Physiology, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
Kang, Da-Won (Department of Physiology, School of Medicine and Institute of Health Sciences, Gyeongsang National University)
Publication Information
Journal of Embryo Transfer / v.24, no.1, 2009 , pp. 57-64 More about this Journal
Abstract
This study was carried out to investigate expression of apoptosis-related differentially expressed gene (DEG) in ovaries of Korean cattle with follicular and luteal cysts and to identify the relationship between cyst and apoptosis using microarray, real-time PCR, TUNEL staining, and Western blot analysis. Microarray data showed that PIK3R2 and AKT1 were significantly up-regulated in follicular cyst, and TNF-RAF2, PRLR, FOXL2, STK4, and COL4A3 were up-regulated whereas INHA, CIDEB, BCL10, and FASLG were down-regulated in luteal cyst. Real-time PCR was performed to validate DEGs altered in luteal cyst. Of nine DEGs, four DEGs down-regulated in luteal cyst showed a positive corelation between microarray data and real-time PCR data. In this study, we focused on INHA, among many DEGs, which was highly down-regulated in both follicular and luteal cysts. Real-time PCR and micro array data showed that INHA was down-regulated by 12.3-fold and by 1.4-fold, respectively, in the bovine follicular cyst. TUNEL assay and Western blot analysis for ERK, JNK, p38, PI3K, and Akt, which were used to detect whether apoptosis is occurred, showed no significant changes in cystic ovaries (p>0.05). In the expression and activity of caspase-3, Bax, Bel-2, and Bel-xL, there was no significant changes between follicular cystic ovary and normal ovary. Rather, the expression levels of PI3K and p-Akt were decreased in follicular cystic ovary. These results suggest that deficiency of apoptosis in cystic ovary is associated with decreased expression of apoptotic effectors.
Keywords
Korean cattle; microarray; follicular cyst; luteal cyst; apoptosis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Billiar RB, Zachos NC, Burch MG, Albrecht ED and Pepe GJ. 2003. Up-regulation of alpha-inhibin expression in the fetal ovary of estrogensuppressed baboons is associated with impaired fetal ovarian folliculogenesis. Biol. Reprod. 68:1989-1996
2 Costanzo LS. 2006. Physiology, 3e. Elsevier Inc.
3 Elvin JA, Yan C, Wang P, Nishimori K and Matzuk MM. 1999. Molecular characterization of the follicle defects in the growth differentiation factor 9-deficient ovary. Mol Endocrinol. 13:1018-1034   DOI   ScienceOn
4 Lopez-Diaz MC and Bosu TK. 1992. A review and an update of cystic ovarian degeneration in ruminants. Theriogenology 37:1163-1183   DOI   ScienceOn
5 Margalit KA, Cowan RG, Harman RM and Quirk SM. 2005. Apoptosis of bovine ovarian surface epithelial cells by Fas antigen/Fas ligand signaling. Reproduction 130:751-758   DOI   ScienceOn
6 Clarke PG and Clarke S. 1995. Historic apoptosis. Nature 378 (6554):230   DOI   ScienceOn
7 Jakimiuk AJ, Weitsman SR, Navab A and Magoffin DA. 2001. Luteinizing hormone receptor, steroidogenesis acute regulatory protein and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries. J. Clin. Endocrinol. Metab. 86:1318-1323   DOI   ScienceOn
8 Jolly PD, Tisdall DJ, Heath DA, Lun S and McNatty KP. 1994. Apoptosis in bovine granulosa cells in relation to steroid synthesis, cyclic adenosine 3',5'-monophosphate response to follicle-stimulating hormone and luteinizing hormone and follicular atresia. Biol Reprod 51:934-944   DOI   ScienceOn
9 최창용, 손동수, 최규찬, 최창열, 최선호, 김현종, 조상래, 허창기, 강다원. 2006. 한우 번식우 사육 농가의 번식 장애 실태 조사. J. Emb. Trans. 21(4):331-338   과학기술학회마을
10 Peter AT and Dhanasekaran N. 2003. Apoptosis of granulosa cells: a review on the role of MAPK-signalling modules. Reprod. Domest. Anim. 38:209-213   DOI   ScienceOn
11 Isobe N and Yoshimura Y. 2000. Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows. Theriogenology 54: 1159-1169   DOI   ScienceOn
12 Isobe Nand Yoshimura Y. 2000a. Localization of apoptotic cells in the cystic ovarian follicles of cows: a DNA-end labeling histochemical study. Theriogenology 53:897-904   DOI   ScienceOn
13 Isobe N and Yoshimura Y. 2007. Deficient proliferation and apoptosis in the granulosa and theca interna cells of the bovine cystic follicle. Journal of Reproduction and Development 53:1119-1124   DOI   ScienceOn
14 Casey OM, Fitzpatrick R, McInerney JO, Morris DG, Powell Rand Sreenan JM. 2004. Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs. Biochimica et Biophysica. 1679: 10-17   DOI   ScienceOn
15 Hakuno N, Koji T, Yano T, Kobayashi N, Tsutsumi O, Taketani Y and Nakane PK. 1996. Fas/APO-1/CD95 system as a mediator granulosa cell apoptosis in ovarian follicle atresia. Endocriology 137:1938-1948   DOI   ScienceOn
16 Hughes FM and Gorospe WC. 1991. Biochemical identification of apoptosis (programmed cell death) in granulosa cells: evidence for a potential mechanism underlying follicular atresia. Endocrinology 129:2415-2422   DOI   ScienceOn
17 Garrett WM and Guthrie HD. 1996. Expression of androgen receptors and steroidogenic enzymes in relation to follicular growth and atresia following ovulation in pigs. Biol. Reprod. 55:949-955   DOI   ScienceOn
18 Peter AT. 2004. An update on cystic ovarian degeneration in cattle. Reprond. Domest. Anim. 39:1-7   DOI   ScienceOn
19 박수봉, 손준규, 박성재, 백광수, 전병순, 안병석, 김현석, 박춘근. 2006. 젖소의 분만 후 비정상적 난소 주기가 번식 성적에 미치는 영향. Reprod. Dev. Biol. 30:213-218
20 최창용, 손동수, 조상래, 김현종, 최선호, 강다원. 2008. 번식장애 한우의 혈액요도태질소 및 성 스테로이드 호르몬 농도 변화. J. Emb. Trans. 23(1):59-64
21 Goravanahally MP, Salem Mohamed, Yao Jianbo, Inskeep EK, and Flores Jorge A. 2009. Differential gene expression in the bovine corpus luteum during transition from early to mid-phase and its potential role in acquisition of luteolytic sensitivity to prostaglandin $\F_2$ Alpha. Biol. Reprod. in press   DOI   ScienceOn
22 Hussein MR. 2005. Apoptosis in the ovary: molecular mechanisms. Human Reproduction Update. 11:162-178   DOI   ScienceOn
23 Lamming GE and Darwash AO. 1998. The use of milk progesterone profiles to characterise components of subfertility in milked dairy cows. Anim Reprod Sci. 52:175-190   DOI   ScienceOn
24 Thornberry NA and Lazebnik Y. 1998. Caspases: Enemies within. Science 281:1312-1316   DOI   ScienceOn