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Chromosomal Analysis of Hanwoo Embryos by In Vitro Culture Condition  

Choi, S.H. (Animal Genetic Resources Station, NIAS, RDA)
Cho, S.R. (Animal Genetic Resources Station, NIAS, RDA)
Han, M.H. (Animal Genetic Resources Station, NIAS, RDA)
Kim, H.J. (Animal Genetic Resources Station, NIAS, RDA)
Choe, C.Y. (Animal Genetic Resources Station, NIAS, RDA)
Son, D.S. (Animal Genetic Resources Station, NIAS, RDA)
Chung, Y.G. (ET Biotech)
Kim, S.K. (Chungnam National University)
Sohn, S.H. (Jinju Industrial University)
Publication Information
Journal of Embryo Transfer / v.22, no.2, 2007 , pp. 137-141 More about this Journal
Abstract
Antioxidants were well known to be essential supplements in the complex media and serve as a reservoir of oxygen. In this study, Hanwoo COCs (cumulus oocytes complexes) were matured and developed in L-cysteine-TCM199 and analyzed metaphase chromosome. Maturation rate of Hanwoo COCs were 73.4%, 94.6% in 0.1% PVA, 0.1 mM L-cysteine, respectively and showed significantly different between the treatments (p<0.05). Blastocyst formation were revealed 20.3%, 10.0% in 5% FBS+TCM199, 0.1 mM L-cysteine+1% BSA, respectively. There were no significant difference among treatment groups. Metaphase chromosome were showed 18.3%, 12.0% in 5% FBS-TCM199, 0.1 mM L-cysteine, respectively and analyzable chromosome were 6.1%, 4.0% and had no differences between the treated groups. In the case of in vitro developmental stages, metaphase chromosome were showed 18.3%, 12.0% in $4{\sim}16$ cells stage, 43.1%, 13.0% in morulae stage and 94.8%, 100.0% in blastocyst stage. These results suggested L-cysteine has beneficial role for in virto maturation and development in Hanwoo COCs.
Keywords
in vitro; embryo; chromosome; Hanwoo;
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