Browse > Article

Development of Porcine Embryos Following Intracytoplasmic Sperm Injection I. Effect of Activation and Sperm Capacitation  

Moon S. J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Ahn S. J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Kang M. J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Kim K. H. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Publication Information
Journal of Embryo Transfer / v.20, no.3, 2005 , pp. 201-206 More about this Journal
Abstract
This study was conducted to investigate the effects of oocyte activation after ICSI and of capacitation of insemination sperm before ICSI in Swine. There was no significant difference on cleavage rate and blastocyst developmental rate treated with ethanol, cycloheximide, or ethanol and cycloheximide jointly between treatment and control groups. However, significantly difference was found on cleavage rate and blastocyst developmental rate treated with caffeine and Ca-ionophore on capacitation of insemination sperm before ICSI (p<0.05). There was no significant difference on pronuclear formation rate and total oocyte activation rate treated with oocyte activation after ICSI between treatment and control groups, but was significant difference on pronuclear formation rate and total oocyte activation rate treated with capacitation treat of sperm (p<0.05).
Keywords
porcine; ICSI; activation; sperm capacitation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Barros A, Sousa M, Olivera C, Silva J, Almeida V and Beires J. 1997. Pregnancy and birrh after intracytoplasmic sperm injection with totally immotile sperm recovered from the ejaculate. Fertil. Steril., 61(6):1091-1094   DOI   ScienceOn
2 Hamano KLX, Fuauchi K, Furudata M and Yoshiaki M. 1999. Gender preselection in cattle with intracytoplasmically injected, flow cytomertrically stored sperm heads. Biol. Repod., 60: 1194-1197   DOI   ScienceOn
3 Kim NH, Lee JW, Jun SH, Lee HT and Chung KS. 1998. Fertilization of porcine oocytes following intracytoplasmic spermatozoon or isolated sperm head injection. Mol. Reprod. Dev., 51 :436-444   DOI   ScienceOn
4 Lacham Kaplan O and Triunson A. 1995. Intracytoplasmic sperm injection in mice : increased fertilization and development to term after induction of the acrosome reaction. Hum. Repod., 10 (10):2642-2649   DOI
5 Ahmadi A, Bongso A and Ng SC. 1996. Intracytoplasmic injection of human sperm into the hamster oocyte(hamster ICSI as-say) as a test for fertilizing capacity of the male-factor sperm. J. Assist. Reprod. Genet., 13:647-651   DOI   PUBMED
6 Grondahl G, Hansen TH, Hossaini A, Heinze I, Gve T and Hyttel P. 1997. Intracytoplasmic sperm injection of in-vitro matured equine oocytes. Biol. Reprod., 57:1495-1501   DOI   ScienceOn
7 Calvin HI and Bedford JM. 1971. Formation of disulfied bonds in the nucleus and accessary structures of mammalian spermatozoa during maturation in the epididymis. J. Reprod. Fertil. Supple., 13:65-75
8 Kim NH, Shin JS, Kim C, Jun SH, Lee HT and Chung KS. 1999. Fertilization and in vitro development of porcine oocytes following intracytoplasmic injection of round spermatid or round spermatid nuclei. Theriogenology, 51:1441-1449   DOI   ScienceOn
9 Hoover L, Baker A, Check JH, Lurie D and Summers D. 1997. Clinical outcome of crypreserved human pronuclear stage embryos resulting from intracytoplasmic sperm injection. Fertil. Steril., 67(4):621-624   DOI   ScienceOn
10 Martin MJ. 2000. Development of in vivo-matured porcine oocytes following intracyto-plasmic sperm injection. Biol. Repord., 63:109-112   DOI   PUBMED   ScienceOn
11 Kimura Y and Yanagimachi R. Intracyto-plasmic sperm injection in the mouse. Biol. Repord., 52:709-720   DOI   ScienceOn
12 Catt SI, Catt JW, Gomez MC, Maxwell WMC and Evans G. 1996. The birth of a male lamb derived from an in vitro matured oocyte fertilized by intra-cytoplasmic injection of a single presumptive male sperm. Vet. Rec., 139:494-495   DOI   ScienceOn
13 Catt JW and Rhodes SL. 1995. Comparative intracytoplasmic injection(ICSI) in human and domestic species. Rephod. Fertil. Dev., 7:161-167   DOI   ScienceOn
14 Motoshi M, Koto K, Tomita K, Ookutsu S and Nakanishi Y. 1996. Examination of the safety of intracytoplasmic injection procedures by using bovine zygotes. Hum. Repod., 11(3):618-320
15 Olson GE, Hamilton DW and Fawcett DW. 1976. Isolation and characterization of the perforatirium of rat spematozoa. J. Reprod. Fertil., 47: 293-297   DOI   ScienceOn
16 Goneg MC, Catt JW, Evans G and Maxwell WMC. 1998. Cleavage, development and competence of sheep embryos fertiliged by intracytoplasmic sperm injection and in vitro fertiliged. Theriogenology., 49:1143-1154   DOI   ScienceOn