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Effect of Culture Medium, Temperature and Coculture on Culture of Immature Porcine Spermatogonia Cells  

Kim H. S. (National Livestock Research Institute)
Cho S. R. (National Livestock Research Institute)
Choi S. H. (National Livestock Research Institute)
Han M. H. (National Livestock Research Institute)
Son D. S. (National Livestock Research Institute)
Ryu I. S. (National Livestock Research Institute)
Kim I. C. (National Livestock Research Institute)
Lee J. H. (National Livestock Research Institute)
Kim I. H. (Chungbuk National University)
Im K. S. (Seoul National University)
Publication Information
Journal of Embryo Transfer / v.20, no.1, 2005 , pp. 35-41 More about this Journal
Abstract
This study was carried out for development of effective preservation on animal genetic resources. Spermatogonia cells are the germline stem cells and they can be restored to adult animal with proliferation and differentiation intentionally. When the spermatogonia cells were purified from seminiferous tubules and were cultured at $32^{\circ}C$, the cells were actively proliferated. The culture medium consisted of TCM199 plus $10\%$ FCS and coculture with Sertoli cells supported cultivation of spermatogonia cells. By passing 40 days of incubation, spermatogonia cells formed the germline colony or shape of ES-like colony or reconstruction of pseudo-seminifcrous tubule shape. At 40 days, the cultured cells were no sign for differentiation to spermatocyte or spermatid. The experiment of induced differentiation of this cells is needed.
Keywords
spermatogonia; coculture; medium; temperature; porcine;
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