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Effects of Serum and Gonadotropins in In-Vitro Maturation Medium on Nuclear Maturation, Development and Cell Numbers of Korean Native Cow Embryos  

Park Y. S. (경상북도축산기술연구소)
Kim J. M. (포천중문의과대학 생리학교실)
Park H. D. (대구대학교 식품생명공학부)
Publication Information
Journal of Embryo Transfer / v.19, no.3, 2004 , pp. 229-237 More about this Journal
Abstract
The main objective of this study was to examine the effects of serum and gonadotropins supplement during in vitro maturation(IVM) of bovine oocytes on nuclear maturation and embryo development, and we also examine the cell number. 1 . The first polar body(PB) extrusion rates of Korean native cow(KNC) oocytes matured in medium with FBS or gonadotropins were similar among treatment groups. The development rate to the blastocyst stage was significantly higher in the group of both supplement FBS and gonadotropins(26.0％) than in the group of non-supplement(9.9％) and gonadotropins (12.0％). The numbers of inner cell mass (ICM) and trophectoderm (TE) cells and total cell numbers of blastocysts were highest in the group of both supplement FBS and gonadotropins, and the number of ICM cells was increased by FBS supplementation (p<0.05). 2. The PB extrusion rates of KNC oocytes matured in medium with FBS in the different duration of IVM was significantly higher in the 0-18hr(63.1％) and in the 9-18hr(63.4％) group than in the 0-9hr.(37.4％) group (p<0.05). The embryo development rates did not differ among treatment groups. The numbers of TE cells and total cell numbers of blastocysts were similar among treatment groups, but the number of ICM cells of the 0-18h. group were significantly higher than the other treatment groups (p<0.05). The results indicate that although TCM199 alone can support bovine oocyte maturation and development to the blastocyst stage, a high quality of blastocysts can be produced from oocytes matured in medium containing serum and gonadotropins.
Keywords
in vitro maturation; 1st polar body; embryo development; cell number;
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1 Abeydeera LR, Wang WH, Cantley TC, Rieke A and Day BN. 1998. Coculture with follicular shell pieces can enhance the developmental competence of pig oocytes after in vitro fertilization: relevance to intracellular glutathione. Biol. Reprod., 58:213-218   DOI   ScienceOn
2 Hunter MG. 2000. Oocyte maturation and ovum quality in pigs. Rev. Reprod., 5:122-130   DOI   PUBMED   ScienceOn
3 Park YS, Choi SH, Han JC, Park HD and Byun MD. 2002. Effects of maturation time on in-vitro production of Korean Native Cow embryos. Korean J. Anim. Reprod., 27:35-44
4 Sirard MA, Florman HM, Leibfried-Rutledge ML, Barnes FL, Sims ML and First NL. 1989. Timing for nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. Biol. Reprod., 40:1257-1263   DOI   ScienceOn
5 Linder GE and Wright RW. 1983. Bovine embryo morphology and evaluation. Theriogenology, 20:407-416   DOI   ScienceOn
6 Lonergan P, Monaghan P, Rizos D, Boland MP and Gordon I. 1994. Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization and culture in vitro. Mol. Reprod. Dev., 37:48-53   DOI   ScienceOn
7 Gardner DK and Lane M. 1993. Embryo culture systems. In: Trounson A, Gardner DK (eds.), Handbook of In vitro Fertilization. Boca Raton, FL : CEC Press; 85-114
8 Rebecca LK, Michelle L and Barry DB. 1999. Developmental competence and metabolism of bovine embryos cultured in semi defined and defined culture media. Biol. Reprod., 60:1345- 1352   DOI   ScienceOn
9 Ward F, Enright B, Riaos D, Boland M and Lonergan P. 2002. Optimization of in vitro bovine embryo production: effect of duration of maturation, length of gamete co-incubation, sperm concentration and sire. Theriogenology, 57:2105-2117   DOI   ScienceOn
10 Park YS, Kim SS, Choi SH, Park NC, Byun MD and Park HD. 2004. Effects of amino acid in in-vitro maturation medium on nuclear maturation and embryo development of Korean Native Cow. Reprod. Deve. Biol., 28:29-38
11 Lee ES and Fukui Y. 1996. Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by vitro-produced bovine morulae and blastocysts. Biol. Reprod. 55:1383-1389   DOI   ScienceOn
12 Ali A and Sirard MA. 2002. Effect of the absence or presence of various protein supplements on further development of bovine oocytes during in vitro maturation. Biol. Reprod., 66:901-905   DOI   ScienceOn
13 Pinyopummintr T and Bavister BD. 1991. In vitro matured/in vitro fertilized bovine oocytes can develop into morulae/blastocysts in chemically defined protein-free culture medium. Biol. Reprod., 45:736-742   DOI   ScienceOn
14 Choi SH, Ryu IS, Kim IH, Park SB, Yeon SH, Jin HJ, Suh CS and Son DS. 1999. Effects of in vitro maturation condition on bovine IVF embryos development. Korean J. Emb. Trans., 14: 113-119
15 Kito S and Bavister BD. 1991. Male pronuclear formation and early embryonic development of hamster oocytes matured in vitro with gonadotropins, amino acid and cysteamine. J. Reprod. Fertil., 110:35-46
16 Watson AJ, Sousa P, Caveney A, Barcroft LC, Natale D, Urquhart J and Westhusin ME. 2000. Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis. Biol. Reprod., 62:355-364   DOI   ScienceOn
17 Blondin P and Sirard MA. 1995. Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes. Mol. Reprod. Dev., 41:54-62   DOI   ScienceOn
18 Pinyopummintr T and Bavister BD. 1994. Development of bovine embryos in a cell-free culture mdrium: effects of type of serum, timing of its inclusion and heat inactivation. Theriogenology, 41:1241-1249   DOI   ScienceOn
19 Lim JM, Lee BC, Lee ES, Chung HM, Ko JJ, Park SE, Cha KY and Hwang WS. 1999. In vitro maturation and in vitro fertilization of bovine oocytes cultured in a chemically defined, protein- free medium: effects of carbohydrates and amino acids. Reprod. Fertil. Dev., 11:127-132   DOI   ScienceOn
20 Steeves TE and Gardner DK. 1999. Temporal and differential effects of amino acids on bovine embryo development in culture. Biol. Reprod., 61:731-740   DOI   ScienceOn
21 Nagai T. 2001. The improvement of in vitro maturation systems for bovine and porcine oocytes. Theriogenology, 55:1291-1301   DOI   PUBMED   ScienceOn
22 Fukui Y and Ono H. 1989. Effects sera, hormonse and granulosa cells added to culture medium for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fertil., 86:501-506   DOI
23 Papaioannou VE and Ebert KM. 1988. The preimplantation pig embryo: cell number and allocation to trophectoderm and inner cell mass of the blastocyst in vivo and in vitro. Development, 102:793-803
24 Yang NS, Lu KH and Gordon I. 1990. In vitro fertilization (IVF) and culture(IVC) of bovine oocytes from stored ovaries. Theriogenology, Abst., 33:352   DOI   ScienceOn
25 Geshi M, Yonai M, Sakaguchi M and Nagai T. 1999. Improvement of in vitro co-culture systems for bovine embryos using a low concentration of carbon dioxide and medium supplemented with $\beta$ -mercaptoethanol. Theriogenology, 51:551-558   DOI   ScienceOn
26 Gardner DK. 1994. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biol Int., 18:1163-1179   DOI   PUBMED   ScienceOn
27 Kim DJ, Chung HJ, Kim DH, Uhm SJ, Lee HT and Chung KS. 2001. Effects of FSH and LH on maturation of bovine preantral follicle. Korean J. Animal Reprod., 25:101-111
28 Edwards RG. 1965. Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature, 208:349-51   DOI   PUBMED   ScienceOn
29 Iwasaki S, Yoshida N, Ushijima H, Watanabe S and Nakahara T. 1990. Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo. J. Reprod. Fertil., 90:279-84   DOI
30 Behbooi E, Anderson GB, Bondurant RH, Cargill SL, Kreusher BR, Medrano JF and Murlay JD. 1995. Birth of large calves that developed from in vitro-derived bovine embryos. Theriogenology, 44:227-232   DOI   ScienceOn
31 Choi YH, Carnevale EM, Seidel GE and Squires EL. 2001. Effects of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology, 56:661-670   DOI   ScienceOn
32 Trounson A, Anderiesz C and Jones G. 2001. Maturation of human oocytes in vitro and their developmental competence. Reproduction, 121: 51-75   DOI   PUBMED
33 Eppig JJ, Schroeder AC and O'Brien MJ. 1992. Developmental capacity of mouse oocytes matured in vitro: effects of gonadotrophic stimulation, follicular origin and oocyte size. J. Reprod. Fertil., 95:119-127   DOI
34 Khatir H, Lonergan P and Mermillod P. 1998. Kinetics of nuclear maturation and protein profiles of oocytes from prepubertal and adult cattle during in vitro maturation. Theriogenology, 50:917-929   DOI   ScienceOn