Browse > Article

Effects of Development and Viability of Pig Oocytes Matured in Defined Medium Containing PVA, PVP and pFF  

Kim I. D. (단국대학교 생명자원과학대학 동물자원학과)
Kim S. N. (단국대학교 생명자원과학대학 동물자원학과)
Han S. K. (단국대학교 생명자원과학대학 동물자원학과)
Seok H. B. (단국대학교 생명자원과학대학 동물자원학과)
Publication Information
Journal of Embryo Transfer / v.19, no.3, 2004 , pp. 219-227 More about this Journal
Abstract
This study was conducted to develop a serum-free, defined medium of IVM of pig oocytes. The TCM-199 with supplemented with polyvinylalcohol(PVA), polyvinylpyrrollidone(PVP) and porcine follicular fluid(pFF) were used as basal medium. The effects of the these additives on the rates of maturity and development under in-vitro fertilization and in vitro culture were examined and subsequently considered on the possibilities be sustituted for the bovine serum albumin(BSA). Maturation rate of pig oocytes in IVM media containing PVA(82.4％), pFF(89.4％) and BSA(90.0％) were significantly higher(P<0.05) than that of PVP(78.6％). Cleavage rate after IVF of PVP(64％) was significantly lower(P<0.05) than these of PVA(73％), pFF(77％) and BSA(73％) supplements. in vitro development rates to morulae and blastocyst on PVP(54％) were also significantly lower(P<0.05) than these of the supplements of PVA(63％), pFF(69％) and BSA(65％). In comparison of maturation and fertilization rates of pig oocytes in each supplements, the maturity rates of PVA(82.4％), pFF(89.4％) and BSA(90.0％) were significantly lower(P<0.05) than that of PVP(72.4％) and while, the fertilization rates of pFF(87.1％) and BSA(89.1％) were significantly higher(P<0.05) than these of PVA(78.0％) and PVP(70.6％). It may be concluded that PVA and pFF can be substituted far BSA in medium for culturing pig oocytes; however, it may be considered that PVP were limited to for BSA in the in vitro culture of the embryos.
Keywords
pig oocytes; PVA; PVP; pFF; BSA; in vitro culture;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Abeydeera LR and Day BN. 1997. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-Buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod., 57:729-734   DOI   ScienceOn
2 Ayoub MA and Hunter AG. 1993. Inhibitory effect of bovine follicular fluid on in vitro maturation of bovine oocytes. J. Dairy Sci., 76:95-100   DOI   ScienceOn
3 Coy P, Martinez E, Ruiz S, Vazquez JM, Roca J, and Matas C. 1993. Sperm concentration influences fertilization and male pronuclear formation in vitro in pig. Therioginology, 40:539- 546   DOI   ScienceOn
4 Fukui Y and Sakuma Y. 1980. Maturation of bovine oocytes cultured in vitro : relation to ovarian activity. follicular size and the presence or absence of cumulus cells. Biol. Reprod., 22: 669-673   DOI
5 Leibfried ML and First NL. 1979. Characterization of bovine follicular oocytes and their ability to matured in vitro. J. Anim. Sci., 48:76-86   DOI
6 Long CR, Dobrinsky JR and Johnson LA. 1999. In vitro production of pig embryos: comparisons of culture media and boars. Theriogenology, 51: 1375-1390   DOI   ScienceOn
7 Ogawa T, Ono T and Marrss RP. 1987. The effect of serum fractions on single-cell mouse embryos in vitro. J. Vitro Fertil. Embryo Transfer, 4:153-158   DOI   ScienceOn
8 Pincus G and Enzmann EV. 1935. The comparative behaviour of mammalian eggs in vivo and in vitro. J. Exp. Med., 62:655-657
9 Saito H, Berger T, Mishell DR Jr and Marrs RP. 1984. The effect of serum fractions on embryo growth. Fetil. Steril., 41:761-765   DOI
10 Yamashita S and Hoshi H. 1996. Bovine blastocyst formation from IVM/IVF produced zygotes in serum and serum free medium. Theriogenology, 46(1):197 abstract
11 Dumoulin JC, Bergers-Janssen JM, Pieters MH, Enginsu ME, Geraedts JP and Evers JL. 1994. The protective effects of polymers in the cryopreservation of human and mouse zonae pellucidae and embryos. Fertil Steril., 62:793- 798   DOI
12 Hong JY, Yong HY, Lee BC, Hwang JM and Lee ES. 2004. Effects of amino acids on maturation, fertilization and embryo development of pig follicular oocytes in two IVM media., 62:1473-1482
13 Biggers JD, Summers MC and McGinnis LK. 1997. Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos. Human Repord. Update 3(2):125-135   DOI   ScienceOn
14 Funahashi H, Cantley T, Stumpf TT, Terlouw SL, Rieke A, and Day BN. 1994b. In vitro development of in vitro-matured pig oocytes following chemical activation or in vitro fertilization. Biol. Reprod., 50:1072-1077   DOI   ScienceOn
15 Funahashi H, Kim NH, Stumpf TT, Cantley T and Day BN. 1996. presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of pig oocytes. Biol. Reprod., 54: 1412-1419   DOI   ScienceOn
16 Sirard MA and Bilodeau S. 1990. Granulosa cells ingibit the resumption of meiosis in bovine oocytes in vitro. Biol. Reprod., 43:777-783   DOI   ScienceOn
17 Brackett B and Zuelke K. 1993. Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology, 39:43-64   DOI   ScienceOn
18 Funahashi H, Stumpf TT, Terlouw SL, Cantley T, Rieke A and Day BN. 1994a. Developmental ability of pig oocytes matured and fertilized in vitro. Theriogenology, 41:1425-1433   DOI   ScienceOn
19 Takagi Y, Mori K, Tomizawa M, Takahashi T, Sugawara S and Masaki J. 1991. Development of bovine oocytes matured, fertilized and cultured in a serum-free, chemically defined medium. Theriogenology, 35:1197-1207   DOI   ScienceOn
20 Eckert J and Niemann. 1995. In vitro maturation, fertilization and culture to blastocysts of bovine oocytes in protein-free media. 43:1211-1225   DOI   ScienceOn
21 Chatot CS, Klein NW, Clapper ML, Resor SR, Singer WD, Russman BS, Holmes GL, Mattson RH and Cramer JA. 1984. Human serum teratogenicity studied by rat embryo culture: epilepst, anticonvulasant druge and nutrition, Epilepsia, 25:205   DOI   ScienceOn
22 SAS/STAT. 1996. SAS user guide. release 6.12 edition, SAS Inst. inc., Cary NC., USA
23 Dekel N and Phillips DM. 1979. Maturation of the rat cumulus oophorus : a scanning microscopic study. Biol. Reprod., 21:9-18   DOI   ScienceOn
24 Vatzias G, Hagen DR. 1999. Effects of porcine follicular fluid and oviduct-conditioned media on maturation and fertilization of porcine oocytes in vitro. Biol Reprod., 60:42-48   DOI   ScienceOn
25 Wang WH, Niwa K and Okuda K. 1991. In vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. J. Reprod. Fertil., 93:491-496   DOI
26 Chen L, Russel PT and Larsen WJ. 1993. Functional significance of cumulus expansion in the mouse:roles for the preovulatory syntheses of hyaluronic acid within the cumulus mass. Mol. Reprod. Dev., 34:87-93   DOI   ScienceOn
27 Iritani A, Niwa K and Imai H. 1978. Sperm penetration of pig follicular oocytes matured in culture. J. Reprod. Fert., 54:379-383   DOI
28 Mattioli M, Ealeati ML and Seren E. 1989. Developmental competence of pig oocyte maturation fertilized in vitro. Theriogenology, 31: 1201-1207   DOI   ScienceOn
29 Gardner DK. 1994. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Int., 18:1163-1179   DOI   PUBMED   ScienceOn
30 Marchal R, Feugang JM, Perreau C, Venturi M, Terqui M and Mermillod P. 2001. Meiotic and development competence of prepubertal and adult swine oocytes. Theriogenology, 56:17-29   DOI   ScienceOn
31 Yoshida M, Ishizaki Y and Kawagishi H. 1990. Blastocyst formation by pig embryos resulting from in vitro fertilization of oocytes matured in vitro. J. Reprod. Fertil., 88:1-8   DOI
32 Choi SH, Yang BC, Kim IH, Son DS, Lee HJ, Lee KW, Park MK, Kim KN and Chung YC. 1997. Effects of serum on in vitro maturation of Korean native cattle oocytes. Theriogenology, 47(1):187   DOI   ScienceOn
33 Kim KS, Mitsumizo N, Fujita K and Utsumi K. 1996. The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos. 45:787-799   DOI   ScienceOn
34 Shamsuddin M, Larsson B, Gustaffsson H and Todriguez-Martinez H. 1994. A serum-free, cell-free culture system for development of bovine one-cell embryos up to blastocyst stage with improved viability. Theriogenology, 41: 1033-1043   DOI   ScienceOn
35 Romero A and Seidel GE Jr. 1994. Effects of bovine follicular fluid on maturation of bovine oocytes. Theriogenology, 41:383-394   DOI   ScienceOn
36 Ashwood-Smith MJ and Warby C. 1971. Studies on the molecular weight and cryoprotective properties of polyvinylpyrrolidone and dextran with bacteria and erythrocytes. Cryobiology, 8:453-464   DOI   ScienceOn
37 Edwards RG. 1965. Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature, 208:349-351   DOI   PUBMED   ScienceOn
38 Cholewa JA and Whitten WK. 1970. Development of two-cell mouse embryos in the absence of a fixed-nitrogen source. J. Reprod. Fert., 22:553-555   DOI
39 Abeydeera LR, Wang WH, Cantley TC, Rieke A, Murphy CN, Prather and Day BN. 2000. Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology, 54:787-797   DOI   ScienceOn
40 Pinyopummintr T and Bavister BD. 1994. Development of bovine embryos in a cell-free culture medium: Effects of type of surum, timing of its inclusion and heat inactivation. Theriogenology, 41:1241-1249   DOI   ScienceOn
41 Motlik J and Fulka J. 1974. Fertilization of pig follicular oocytes cultivated in vitro. J. Reprod. Fert., 36:235-237   DOI
42 Kim HS, Lee GS, Hyun SH, Lee SH, Nam DH, Jeong YW, Kim S, Kang SK, Lee BC and Hwang WS. 2004. Improved in vitro development of porcine embryos with different energy substrates and serum. 61:1381-1393   DOI
43 Kikuchi K, Nagai T, Kashiwazaki N, Ikeda H, Nouguchi J, Shimada A, Soloy E, and Kaneko H. 1998. Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4$^{\circ}C$. Theriogenology, 50: 615-623   DOI   ScienceOn
44 Kane MJ. 1987. Minimal nutrient requirements for culture of one-cell rabbit embryos. Biol. Reprod., 37:775-778   DOI   PUBMED   ScienceOn
45 Lee ES and Fukui Y. 1996. Synergistic effect of alanin and glycine on bovine embryos cultured in a chemically defined medium and animo acid uptake by in vitro-produced bovine morulae and blastocyst. Biol. Reprod., 55(6):1383-1389   DOI   ScienceOn
46 Choi SH, Ryu IS, Kim IH, Park SB, Yeon SH, Jin HJ, Suh SW, Lee CS and Son DS. 1999. Effects of in vitro maturation condition on bovine IVF embryos development. Korean J. Emb. Trans., 14:113-119
47 Coy P, Ruiz S, Romar R, Campos I and Gadea J. 1999. Maturation, fertilization and complete development of porcine oocytes matured under different systems. Theriogenology, 51:799-812   DOI   ScienceOn