Browse > Article

Production of In-Vivo Embryos by Superovulation and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle  

Kim Y. J. (전북대학교 수의과대학)
Song J. W. (전북대학교 수의과대학)
Seo S. H. (전주동물원)
Jeong K. N. (전라북도 축산진흥연구소 종축시험소)
Kim Y. S. (전라북도 축산진흥연구소 종축시험소)
Lee H. R. (전북대학교 수의과대학)
Shin D. S. (전라북도 축산진흥연구소 종축시험소)
Jo S. W. (전라북도 축산진흥연구소 종축시험소)
Kim S. H. (전북대학교 수의과대학)
Publication Information
Journal of Embryo Transfer / v.19, no.3, 2004 , pp. 209-218 More about this Journal
Abstract
To investigate the feasibility of embryo transfer technology to promote productivity of cattle, 36 cows(18 Holstein, 18 Hanwoo) were superovulated. Fresh embryos were transferred to 25 recipients(14 Holstein, 11 Hanwoo), whereas frozen embryos were transferred to 17 recipients(10 Holstein, 7 Hanwoo). Two embryos were transferred at a time to 13 recipients(9 Holstein, 4 Hanwoo) to produce twin calves. 1. 75.0％ of donor cattle were reacted to hormonal treatment far superovulation. 2. The rate of embryo recovery by non-surgical method for Holstein and Hanwoo was 90.4 and 95.8％ in comparison with numbers of corpus luteum. 3. Of all the ova collected non-surgically, the rate of viable blastocyst was 66.4％ and the rate of transferrable blastocysts was 48.6％. 4. The rate of embryo collection by one-catheter method was 75.0％. 5. The rate of pregnancy/delivery following embryo transfer with fresh embryos was 60.0％. 6. The rate of pregnancy/delivery following embryo transfer with frozen embryos was 35.3％. 7. In embryo transfer to produce twin calves, the rate of pregnancy/delivery was 28.6％ with fresh embryos and 16.7％ with frozen embryos.
Keywords
superovulation; in-vivo embryo; embryo transfer; fresh embryo; frozen embryo;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Greve T et al. 1979. Morphological evaluation of bovine embryos recovered non-surgically from superovulated dairy cows 6$\frac{1}{2}$ to 7$\frac{1}{2}$. A field study. Ann. Biol. Anim. Biochem. Biophys., 19:1599-1612   DOI
2 Hasler JF et al. 1983. Superovulatory responses of Holstein cows. Theriogenology, 19:83-99   DOI   ScienceOn
3 Hasler JF. 2003. The current status and future of commercial embryo transfer in cattle. Theriogenology, 79:245-264
4 Kanagawa H et al. 1981. Ovarian response after four continuos administration of gonadotrophin in beef cattle. Jpn. J. Vet. Res., 29:1-7   PUBMED
5 Kanagawa H. 1988. Bovine embryo transfer. JICA., 114-115
6 Mapletoft RJ, Lindsell CE and Pawlyshyn V. 1986. Effects of clenbuterol, body condition and non- surgical embryo transfer equipment on pregnancy rates in bovine recipients. Theriogenology, 25:172   DOI   ScienceOn
7 Scanlon PF et al. 1974. Multiple ovulations, multiple pregnancies and multiple births in Irish cattle. J. Irish Dept. Agric. Fish., 70:2-18
8 Shimohira I. 1990. Twinning and in vitro fertilization technology for cattle. JICA., 25-28
9 고광두, 정길생, 이기만. 1981. 한우의 수정란 이식에 관한 연구. 수정란의 비외과적 채취와 이식. 한국축산학회지, 23:331-337
10 김용준, 이창민, 정구남, 등 2003. 성감별된 한우 수정란의 수정란이식. 한국수정란이식학회지, 18(2):97-108
11 Sreenan JM and Beehan D. 1976. Effect of site of transfer on pregnancy and twinning rates following bilateral egg transfer in the cow. J. Reprod. Fertil., 48:223-224   DOI
12 Niemann H, Tenhumserg H, Sacher B and Kruff B. 1985. Pregnancy rates after nonsurgical transfer of cattle embryos frozen and thawed by a field method. Anim. Breed., 53:206-207
13 Donaldson LE. 1985. Matching of embryo stage and grades with recipient oestrus synchrony in bovine embryo transfer. Vet. Rec., 9:489-491
14 Newcomb R et al. 1978. Comparison of the fetal survival rate in heifers after the transfer of an embryo surgically to one uterine horn and non-surgically to the other. J. Reprod. Fertil., 52:395-397   DOI   PUBMED
15 Broadbent PJ, Stewart M and Dolman DE. 1991. Recipient management and embryo transfer. Theriogenology, 27:125-139
16 Newcomb R. 1979. Surgical and non-surgical transfer of bovine embryos. Vet. Rec., 105:432-434   DOI   PUBMED   ScienceOn
17 황환섭, 장현용. 김성곤, 등. 2004. 한우 체외성숙․체외수정란의 수정란이식에 관한연구. 한국수정란이식학회지, 19(1):1-10
18 Nelson LD, Elsden RP and Seidel GE Jr. 1982. Effect of synchrony between estrus cycles of donors and recipients on pregnancy rates in cattle. Theriogenology, 17:101(abstr.)   DOI   ScienceOn
19 Saumande J et al. 1978. Factors affecting the variability of ovulation rates after PMSG stimulation: Control of Reproduction in the Cow. ed. Sreenan JM. 195-224
20 석호봉, 이광원, 신용식 등. 1983. 소의 동결수정란이 수태에 미치는 영향. 한국축산학회지, 24(4): 369-374
21 Putney DJ, Thatcher WW and Drost M. 1988. Influence of environmental temperature on reproductive performance of bovine embryo donors and recipients in the southwest region of the united states. Theriogenology, 30:905-922   DOI   ScienceOn
22 Newcomb R et al. 1980. Fetal survival rate after the transfer of 2 bovine embryos. J. Reprod. Fertil., 59:31-36   DOI   PUBMED
23 Ellington JE, Foote RH, Farrell PB, Hasler JF, Webb J, Henhderson WB and McGrath AB. 1991. Pregnancy rate after the use of a gonadotropin releasing hormone agonist in bovine embryo transfer recipients. Theriogenology, 36: 1035-1042   DOI   ScienceOn
24 Willett FL, Black WG, Casida LE, Stone WH and Buckner PJ. 1951. Successful transplantation of a fertilized bovine ovum. Science, 113:247
25 조충호, 강병규, 최상용, 황우석, 김용준. 1990. 증정 수의산과학, 123-124
26 Dorn CG and Kraemer DC. 1987. Bovine embryo grading. Texas A&M University, 1-17
27 Wright JM. 1985. Commercial freezing of bovine embryos in straw. Theriogenology, 23:17-29   DOI   ScienceOn