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Effect of In-Vitro Fertilization of Porcine Matured Oocytes in Different IVM-IVC Culture Media on the Development of the Embryos  

안미현 (단국대학교 생명 자원과학대학 동물자원과학과)
홍대욱 (단국대학교 생명 자원과학대학 동물자원과학과)
석호봉 (단국대학교 생명 자원과학대학 동물자원과학과)
Publication Information
Journal of Embryo Transfer / v.18, no.3, 2003 , pp. 269-274 More about this Journal
Abstract
This experiment was carried out to investigate the effects of different IVM-IVC culture media factors, such as development rates according to the maturation media, collecting times from slaughter to initiation of incubation and with cumulus cells, on in vitro maturation of oocytes collected from 3∼5mm diameter follicles of the swine abattoir The development rates significantly(p<0.05) higher when the oocytes were matured TCM-199 media than NCSU-23 media. In comparing with TCM199 medium in presence of Earle's salts and Hank's salt, there were no significantly differences between each salt balance in cleaved rate and in number of morulae plus blastocyst. Among 1,455 immature oocytes, 999(68.6％) of oocytes were cleaved. The number of development to the morulae and blastocysts were 617(61.8％) include 62 balstocysts(6.2％).
Keywords
in vitro maturation; TCM199; NCSU23; Earle's solution; Hank's solution; porcine;
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