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Effect of Macromolecules Supplemented to NCSU-23 and PZM Culture Media on in vitro Development of Porcine Embryos  

Kim, Soo (Department of Veterinary Medicine, Seoul National University)
Lee, So-Hyun (Department of Veterinary Medicine, Seoul National University)
Kim, Dae-Young (Department of Veterinary Medicine, Seoul National University)
Kang, Sung-Geun (Department of Veterinary Medicine, Seoul National University)
Lee, Byung-Chun (Department of Veterinary Medicine, Seoul National University)
Hwang, Woo-Seok (Department of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Embryo Transfer / v.18, no.1, 2003 , pp. 35-41 More about this Journal
Abstract
Three experiments were performed to develop an optimal culture system of in-vitro derived porcine embryos. In experiment 1, embryos were cultured in two different basic culture media (NCSU-23 and PZH) supplemented with BSA (4mg/ml), FBS (10%), PVA (3mg/ml). Although no difference on the percentage of embryos developed to blastocysts was found (P>0.05), more 2-cell embryos (59.5% vs. 44.7 to 52.0%) were obtained when embryos were cultured in NCSU-23 added with BSA. In experiment 2, identical treatment was conducted using PZM medium. More (P<0.05) 2-cell embryos (54.4% vs. 40.7 to 44.5%) and blastocysts (12.5% vs. 5.8 to 9.0) were produced when embryos were cultured in PZM added with BSA (4mg/ml). In experiment 3, embryos were cultured in media as follows; 1) NCSU-23 without BSA for 192 hours; 2) NCSU-23 with BSA for 192 hours; 3) NCSU-23 with BSA for 96 h and then subsequently injected with FBS into culture drops. Although no difference on the percentage of embryos developed to blastocysts was found (P>0.05), more 2-cell embryos (63.8 to 69.2% vs. 52.0%) were obtained when embryos were cultured both in NCSU-23 added with BSA throughout culture duration and in FBS supplemented medium. In conclusion, no difference was found when porcine preimplantation embryos were cultured in NCSU-23 added with various macromolecules. However, when PZM medium was used, PZM medium supplemented with BSA showed more potential to support embryo development. Finally, FBS supplemented into culture drop 96h post-insemination did not show any benefits on embryo development.
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1 Abeydeera LR, Wang WH, Prather RS and Day BN. 1998. Maturation of in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro. BioI. Reprod., 58:1316-20   DOI   ScienceOn
2 Biggers JD, Summers MC and McGinnis LK. 1997. Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos. Hum. Reprod. Update., 3:125-135   DOI   ScienceOn
3 Choi YH, Lee BC, Lim JM, Kang SK and Hwang WS. 2002. Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcome. Theriogenology, 58:1187-1197   DOI   ScienceOn
4 Dobrinsky JR, Johnson LA and Rath D. 1996. Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development. BioI. Reprod., 55:1069-1074   DOI   ScienceOn
5 Lee ES and Fukui Y. 1996. Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by vitro-produced bovine morulae and blastocysts. BioI. Reprod., 55: 1383-1389   DOI   ScienceOn
6 Pollard JW, Plante C and Leibi SP. 1995. Comparison of development of pig zygotes and embryos in simple and complex culture media. J. Fertil., 103:331-337   DOI
7 Rath D, Niemann H and Torres CRL. 1995. In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro. Theriogenology, 43:913-926   DOI   ScienceOn
8 Takahashi Y and First NL. 1992. In vitro development of bocine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology, 37:963-978   DOI   ScienceOn
9 Abeydeera LR, Wang WH, Cantley TC, Rieke A, Prather RS and Day BN. 1998. Presence of epidermal growth factor during in vitro maturation pf pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol. Reprod. Dev., 51:395-401   DOI   ScienceOn
10 Karja NW, Otoi T, Murakami M, Yuge M, Fahrudin M and Suzuki T. 2002. Effect of protein supplementation on development to the hatching and hatched blastocyst stages of cat IVF embryos. Reprod. Fertil. Dev., 14: 291-296   DOI   ScienceOn
11 Rizos D, Gutierrez-Adan A, Perez-Garnelo S, De La Fuente J, Boland MP and Lonergan P. 2003. Bovine embryo culture in the presence or absence of serum implications for blastocyst development, cryotolerance, and messenger RNA expression. BioI. Reprod., 68:236-243   DOI
12 Petters RM and Wells KD. 1993. Culture of pig embryos. J. Reprod. Fertil. Suppl., 48:61-73
13 Lane M and Gardner DK. 2000. Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryos. BioI. Reprod., 62: 16-22   DOI   ScienceOn
14 Thompson JG, Bell ACS, Pugh PA and Tervit HR. 1993. Metabolism of pyruvate by pre-elongation sheep embryos and effect of pyruvate and lactate concentrations during culture in vitro. Reprod. Fertil. Dev., 5:417-423   DOI   ScienceOn
15 Wang S, Liu Y, Holyoak GR and Bunch TD. 1997. The effects of bovine serum albumin and fetal bovine serum on the development of pre- and postcleavage-stage bovine embryos cultured in modified CR2 and M199 media. Anim. Reprod. Sci., 48:37-45   DOI   ScienceOn
16 Gandhi AP, Lane M, Gardner DK and Krisher RL. 2001. Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media. Mol. Reprod. Dev., 58:269-275   DOI   ScienceOn
17 Beckmann LS and Day BN. 1993. Effect of medium NaCI concentration and osmolarity ion culture of the early stage porcine embryo and viability of embryos cultured in a selected superior medium. Theriogenology, 39:611-622   DOI   ScienceOn