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Production of Korean Native Cow from Mongolian Cow following Transfer of Vitrified Blastocyst  

Kong, I.K. (Department of Animal Science and Technology, Sunchon National University)
Sanjjav, G. (Department of Physiology, Monogolia State University of Agriculture)
Yang, C.J. (Department of Animal Science and Technology, Sunchon National University)
Cho, S.G. (Department of Animal Science and Technology, Sunchon National University)
Bae, I.H. (Department of Animal Science and Technology, Sunchon National University)
Oh, D.H. (Department of Animal Science and Technology, Sunchon National University)
Publication Information
Journal of Embryo Transfer / v.17, no.2, 2002 , pp. 129-136 More about this Journal
Abstract
The purpose of this study was to investigate the comparison of viability of bovine blastocysts following glass micropipette (GMP) vitrification and the possibility of production of Korean Native Cow ("Hanwoo,"Bos taurus coreanae) following embryo transfer into Mongolia cows (Bos taurus mongolian). The embryos of Korean Native Cow were produced by IVMFC or superovulation methods in Korea, cryopreserved by GMP vitrification, and subsequently trans-ported to Mongolia. The recipient cows were synchronized using a CIDR plus and prostaglandin $F_2\alpha$($PGF_2\alpha$) treatment. To produce in vivo embryos, seven cows were superovulated using FSH and PGF$_2$／sub $\alpha$／ treatment. A total of 64 blastocysts ( $9.1\pm2.94$ per cow) were collected. In vitro embryos were produced using a defined culture system which cleaved in 80.1％ ova (174／217), and developed to blastocyst stage embryos of 40.8％ (71／174). The post-thaw survival rate of in vivo blastocysts (93.7％; 45／48) was significantly higher than that of in vitro blastocysts (82.5％; 52／63, P＜0.05). Embryo transfer was carried out using 8 Mongolian recipient cows and 2 post-thaw blastocysts per recipient. Five of 8 recipients were found pregnant at Day 60 but one abortion occurred by Day 240. Two of offspring were produced from the Mongolian cows at 275 days after embryo transfer. These results indicated that a GMP vitrification method could be used as a cryopreservation technique for in vivo or in vitro bovine blastocysts and produced effectively a Korean Native Cow following embryo transfer into a Mongolian recipient cow.
Keywords
GMP vitrification; bovine; embryo transfer; Korean native cow;
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