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http://dx.doi.org/10.15681/KSWE.2020.36.6.581

High-rate Denitrifying Process Based on Methanol and Characteristics of Organic Carbon Uptake  

Park, Suin (Department of Civil and Environmental Engineering, Pusan National University)
Jeon, Junbeom (Department of Civil and Environmental Engineering, Pusan National University)
Bae, Hyokwan (Department of Civil and Environmental Engineering, Pusan National University)
Publication Information
Journal of Korean Society on Water Environment / v.36, no.6, 2020 , pp. 581-591 More about this Journal
Abstract
In this study, two types of reactors were operated to examine the properties of methanol uptake under the high-rate denitrification process. In a sequencing batch reactor, the denitrifying activity was enriched up to 0.80 g-N/g-VSS-day for 72 days. Then, the enriched denitrifying sludge was transferred to a completely stirred tank reactor (CSTR). At the final phase on Day 46-50, the nitrogen removal efficiency was around 100% and the total nitrogen removal rate reached 0.097±0.003 kg-N/㎥-day. During the continuous process, the sludge settling index (SVI30) was stabilized as 118.3 mL/g with the biomass concentration of 1,607 mg/L. The continuous denitrifying process was accelerated by using a sequencing batch reactor (SBR) with a total nitrogen removal rate of 0.403±0.029 kg-N/㎥-day with a high biomass concentration of 8,433 mg-VSS/L. Because the reactor was open to ambient air with the dissolved oxygen range of 0.2-0.5 mg-O2/L, an increased organic carbon requirement of 5.58±0.70 COD/NO3--N was shown for the SBR in comparison to the value of 4.13±0.94 for the test of the same biomass in a completely anaerobic batch reactor. The molecular analysis based on the 16S rRNA gene showed that Methyloversatilis discipulorum and Hyphomicrobium zavarzinii were the responsible denitrifiers with the sole organic carbon source of methanol.
Keywords
Bacterial community structure; Denitrification; Methanol; Specific denitrification rate;
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