Browse > Article
http://dx.doi.org/10.15681/KSWE.2019.35.3.201

The Component Analysis of the Bioflocculant Produced by Strain YG-02  

Jung, Yeon-Gon (Department of Environment and Energy Engineering, Chonnam National University)
Ko, Joon-Il (Department of Environment and Energy Engineering, Chonnam National University)
Chung, Seon-Yong (Department of Environment and Energy Engineering, Chonnam National University)
Publication Information
Journal of Korean Society on Water Environment / v.35, no.3, 2019 , pp. 201-208 More about this Journal
Abstract
In this study, we analyzed composition of the bioflocculant, which strain YG-02 produces. First, supernatant and suspension from centrifugation of culture fluid of the strain, were used in the flocculation experiment. As a result, the SVI(sludge volume index) added with the suspension, was 182 mL/g, same as the control group with no additive, and the SVI added with supernatant, was 164 mL/g. So, the result above showed that flocculation capacity of the bioflocculant, was dependent on the substance which strain YG-02 produces, not on factors such as the body of germs. As a result of the thermostability test on substances that cause flocculation, the flocculation effect was significantly reduced, compared to the result of the flocculation test, before applying heat to the culture fluid, and it was able to assume that the substance that causes flocculation, was damaged by heat. Additionally, to understand the component of the bioflocculant, analyzation of sugar composition and fatty acid, was conducted. As a result, sugar composition was the polysaccharide consisting of glucose: lactose with molar ratio of 90.75:9.25. Fatty acid content was detected, as 0.0012 g/100g, showing that it contained glycolipid in the bioflocculant. Such results show that the bioflocculant which strain YG-02 produces, is the new bioflocculant, different from bioflocculantstudiedto date.
Keywords
Bioflocculant; Glycolipid; Molar ratio; Polysaccharide;
Citations & Related Records
연도 인용수 순위
  • Reference
1 American Public Health Association, American Water Works Association, and Water Environment Federation (APHA, AWWA and WEF). (1998). Standard methods for the examination of water and wastewater, 20th ed., 2710D Sludge Volume Index, APHA, AWWA and WEF.
2 Bough, W. A. (1975). Reduction of suspended solids in vegetable canning waste effluents by coagulation with chitosan, Journal of Food Science., 40, 297-301.   DOI
3 Bough, W. A., Shewfelt A. L., and Salter. W. A. (1975). Use of chitosan for the reduction and recovery of solids in poultry processing waste effluents, Poultry Science, 54, 992-1000.   DOI
4 Byun, B. K. (1999). Characterization of bioflocculant produced by Bacillus sp. KF0317A, Master Dissertation, Graduate School of Andong National University. [Korean Literature]
5 Giri, S. S., Harshiny, M., Sen, S. S., Sukumaran, V., and Park, S. C. (2015). Production and characterization of a thermostable bioflocculant from Bacillus subtilis F9, isolated from wastewater sludge, Ecotoxicology and Environmental Safety, 121, 45-50.   DOI
6 Jun-ichi, K. and Minoru, T. (1991). Synergister flocculating of the bioflocculant fix extracelluary produced by Norcadia sp., The Journal of General and Applied Microbiology, 37, 447-454.   DOI
7 Jung, Y. G., Ko, J. I., and Chung, S. Y. (2018). Isolation and characterization of bioflocculant producing strain YG-02, Journal of the Korean Society of Urban Environment, 18(3), 251-260. [Korean Literature]   DOI
8 Kim, T. W. (2009). A study on the comparison of coagulation characteristics between CHITOSAN(natural polymer coagulant) and PAC, Master's Thesis, Chonbuk National University. [Korean Literature]
9 Kurane, R. (1994). Purification and characteristion of lipid bioflocculant produced by Rhodococcus erythropolis, Bioscience, Biotechnology, Biochemistry, 58(11), 1977-1982.   DOI
10 Kurane, R. and Nohata, Y. (1991). Microbial flocculation of waste liquids and oil emulsion by a bioflocculant from Alcaligenes latus, Agricultural and Biological Chemistry, 55, 1127-1129.   DOI
11 Kurane, R., Takeda, K., and Suzuki, T. (1986). Screening for and characteristics of microbial flocculants, Agricultural and Biological Chemistry, 50, 2301-2307.   DOI
12 Kwon, G. S. (1992). Characteristics of exopolysaccharide KS-1 produced by Bacillus polymyxa as a mutant, Ph. D. Dissertation, Graduate School of Kon-Kuk University. [Korean Literature]
13 Kwon, G. S., Moon, S. H., Hong, S D., Lee, H. M., Kim, H. S., Oh, H. M., aud Yoon, B. D. (1996). A novel flocculant biopolymer produced by Pestalotiopsis sp. KCTC 8637P, Biotechnology Letters, 18(12), 1459-1464.   DOI
14 Ikeda, F., Shuto, H., Fukui, T.. and Tomita, K. (1982). An extracellular polysaccharide produced by Zoogloea ramigera 115, The FEBS Journal, 123, 437-445.
15 Lee, S. H. (2000). Studies on the bioflocculant from the microorganism Strain TS-49, Ph. D. Dissertation, Pusan National University. [Korean Literature]
16 Parker. D. D. and Munn, C. B. (1984). Increased cell surface hydrophibicity associated with possesion of an addidional surface protein by Aeromonas sp., FEMS Microbiology Letters, 21(2), 233-237.   DOI
17 Takagi, H. and Kadowaki, K. (1985a). Flocculant production by Paecilomyces sp. Taxonomic studies and culture condition for production, Agricultural and Biological Chemistry, 49, 3151-3157.   DOI
18 Salehizadeh, H., and Shojaosadati, S. (2001). Extracellular biopolymeric flocculants: recent trends and biotechnological importance, Biotechnology advances, 19, 371-385.   DOI
19 Suh, H. H. (1995). A study on the bioflocculant from Bacillus sp. DP-152, Ph. D. Dissertation, Graduate School of Kon-Kuk University. [Korean Literature]
20 Seo, H. C. (2011). Purification and characterization of bioflocculant producing from Lactobacillus jensenii YW-33, Korean Journal of Environmental Biology, 29(4), 305-311. [Korean Literature]
21 Takagi, H. and Kadowaki, K. (1985b). Purification and chemical properties of a flocculant produced by Paecilomyces sp., Agricultural and Biological Chemistry, 49, 3159-3164.   DOI
22 Toeda, K. and Kurane, R. (1991). Microbial flocculant from Alcaligenes cupidus KT201, Agricultural and Biological Chemistry, 55(11), 2793-2799.   DOI
23 Zajic, J. E. and Leduy, A. (1973). Flocculant and chemical properties of a polysaccharide from Pullularia pullulans, Applied. Microbiology, 25(4), 628-635.   DOI