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Standardization of Ecotoxicity Assay Method for Heavy Metals using Inhibition of Dehydrogenase Activity  

Oh, Kyoung-Hee (Department of Environmental Engineering, Chungbuk National University)
Han, Ah-Won (Department of Environmental Engineering, Chungbuk National University)
Cho, Young-Cheol (Department of Environmental Engineering, Chungbuk National University)
Publication Information
Journal of Korean Society on Water Environment / v.26, no.4, 2010 , pp. 574-579 More about this Journal
Abstract
In this study the enzyme inhibition method using dehydrogenase which has been popularly used to estimate ecotoxicity was optimized. When three bacterial strains, Escherichia coli HB101, Enterobacter asburiae KCAD-4, and Aeromonas media KCAD-13, were compared, KCAD-4 was considered as the adequate strain to estimate toxicity because of its sensitivity and reproducibility. The optimal bacterial density was estimated as $5.4{\times}10^9CFU/mL$, at which the maximum sensitivity was observed. The phosphate buffer was suitable for the reaction solution. When the reaction times required for inhibition of enzyme activity by contact of toxicants and for reaction of damaged bacteria and substrate were tested, the optimal value was estimated as 20 min and 2 hrs, respectively. It is expected that the optimized conditions can be used to develop the standardized kits to estimate ecotoxicity of heavy metals in effluent from the industrial wastewater treatment facilities.
Keywords
Dehydrogenase; Ecotoxicity; Enzyme inhibition assay; Standardization;
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Times Cited By KSCI : 4  (Citation Analysis)
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