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The Influence of Various Factors upon the Membrane Filter Technique on Raw Water of the Nak-Dong River  

Hyun, Jae-Yeoul (Water Quality Research Institute, Waterworks Headquarters, Daegu Metropolitan City)
Yoon, Jong-Ho (Water Quality Research Institute, Waterworks Headquarters, Daegu Metropolitan City)
Shin, Sang-Hee (Water Quality Research Institute, Waterworks Headquarters, Daegu Metropolitan City)
Kim, Jong-Woo (Water Quality Research Institute, Waterworks Headquarters, Daegu Metropolitan City)
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Abstract
In this study, the membrane filter method was compared to the MPN method for the analysis of total coliforms from raw water using raw test waters and controls including 6 standard strains of coliforms, and the various factors were analyzed on the detection of general and fecal origin coliforms. The range of error rate for the detection of 5 standard strains using the membrane filter and the MPN methods was 0 to 6% and 45 to 133%, respectively. The error rate of the membrane filter method was lower than that of the MPN method. The membrane filter method (m-Endo) showed 10% (11 out of 111) of difference for the detection sensitivity of coliforms isolated from raw water compared to the MPN method (BGLB). The membrane filter method was less affected by the factors including temperature, turbidity, charcoals of powder form, contamination, and reverse pressure. In conclusion, the membrane filter method is a better method for the analysis of total coliforms from raw water than the MPN method, considering the accuracy of detection and the tolerance to various experimental factors.
Keywords
Coliforms; Cross-matching test; Membrane filter technique; MPN method; Nak-Dong river;
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