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Identification of Cryptosporidium in Environmental Sample using Nested PCR-RFLP and DNA Sequencing  

Park, Sangjung (National Institute of Environmental Research)
Jeong, Hyanghee (Nakdong River Basin Environmental Office)
Publication Information
Journal of Korean Society on Water Environment / v.24, no.6, 2008 , pp. 817-822 More about this Journal
Abstract
In order to identify various Cryptosporidium species in environment, nested PCR-RFLP and DNA sequencing method were used. The sensitivity of nested PCR-RFLP based on 18s rRNA gene was shown to 1 oocyst. Therefore, we applied nested PCR-RFLP method to environmental samples. As a result, only 4 samples out of 8 samples confirmed as Cryptosporidium parvum by standard method of Cryptosporidium were identified as Cryptosporidium parvum by nested PCR-RFLP and DNA sequencing method. The rest of 4 samples among 8 samples were identified as Cryptosporidium muris, Cryptosporidium bailey. Therefore, in addition to standard method of Cryptosporidium, supplementary verification through nested PCR-RFLP and DNA sequencing should be needed to give more accurate information about risk of Cryptosporidium.
Keywords
Cryptosporidium bailey; Cryptosporidium muris; Cryptosporidium parvum; DNA Sequencing; Nested PCR-RFLP;
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