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Nitrite Accumulation Characteristics and Quantitative Analyses of Nitrifying and Denitrifying Bacteria in a Sequencing Batch Reactor  

Kim, Dong-Jin (Department of Environmental Sciences and Biotechnology, Hallym University)
Kwon, Hyun-Jin (Department of Environmental Sciences and Biotechnology, Hallym University)
Yoon, Jung-Yee (Department of Environmental Sciences and Biotechnology, Hallym University)
Cha, Gi-Cheol (Department of Environmental Engineering, Yonsei University)
Publication Information
Journal of Korean Society on Water Environment / v.24, no.3, 2008 , pp. 383-390 More about this Journal
Abstract
Recently, the interests on economical nitrogen removal from wastewater are growing. As a method of the novel nitrogen removal technology, nitrogen removal via nitrite pathway by selective inhibition of free ammonia and free nitrous acid on nitrite oxidizing bacteria have been intensively studied. The inhibition effects of free ammonia and free nitrous acid are low when domestic wastewater is used, however, because of its relatively lower nitrogen concentration than the wastewater from industry and landfill, etc. In this study, a sequencing batch reactor (SBR) is proposed for nitrogen removal to investigate the effect of the low nitrogen concentration on nitrite accumulation. Nitrification efficiency reached almost 100% during the aerobic cycle and the maximum specific nitrification rate ($V_{max,nit}$) reached $17.8mg\;NH_4{^+}-N/g\;MLVSS{\bullet}h$. During the anoxic cycle, average denitrification efficiency reached 87% and the maximum specific denitrification rate ($V_{max,den}$) reached $9.8mg\;NO_3{^-}-N/g\;MLVSS{\bullet}h$. From the analysis the main reason of nitrite accumulation in the SBR was free nitrous acid rather than free ammonia. Nitrite accumulation increased with the decrease of organic content in the wastewater and the mechanism is not well understood yet. From the result of fluorescent in situ hybridization, the distribution of nitrite oxidizing bacteria was in equilibrium with ammonium oxidizing bacteria when nitrite accumulation did not occur.
Keywords
Denitrification; Fluorescence in situ hybridization (FISH); Nitrification; Nitrite accumulation; Sequencing batch reactor (SBR);
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