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Investigation of Microbial Communities in the Anammox Reactor Seeded with Sewage Sludge and Anaerobic Granule  

Park, Kyung-Soon (Center for Environmental Technology Research, Korea Institute of Science and Technology)
Bae, Hyokwan (Center for Environmental Technology Research, Korea Institute of Science and Technology)
Chung, Yun-Chul (Center for Environmental Technology Research, Korea Institute of Science and Technology)
Park, Yong Keun (School of Life Sciences and Biotechnology, Korea University)
Jung, Jin-Young (Center for Environmental Technology Research, Korea Institute of Science and Technology)
Publication Information
Journal of Korean Society on Water Environment / v.23, no.3, 2007 , pp. 397-402 More about this Journal
Abstract
Anammox reactor seeded with sewage sludge from RBC reactor and anaerobic granule from full-scale UASB reactor treating distillery wastewater was operated. Mixed granule and suspended sludge in the ammonium oxidizing process were taken and analyzed to investigate microbial community structure by molecular methods such as gene cloning and phylogenetic tree analysis after 250 days of continuous cultivation. The average nitrogen removal rate showed $0.9kg\;N/m^3-day$ after 250 days of continuous operation, then the maximum nitrogen removal rate showd $1.9kg\;N/m^3-day$ when $2.1kg\;N/m^3-day$ of nitrogen loading rate was applied. As results of gene cloning and phylogenetic tree analysis, Three kinds of phylum were found to be Proteobacteria, Acidobacteria and Planctomycetes (anammox bacteria) in mixed granule. Five kinds of phylum were found to be Proteobacteria, Chlorobi, Planctomycetes, Nitrospirae and Verrucomicrobia in suspended sludge. We found planctomycete KSU-1 and putative new anammox bacteria in the reactor. Microbial structure represented different consortia depending on the types of sludge in the anammox reactor.
Keywords
Anaerobic granule; Anammox; Clone library; Distribution; Phylogenetic tree; Sewage sludge;
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Times Cited By KSCI : 1  (Citation Analysis)
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