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http://dx.doi.org/10.11001/jksww.2021.35.6.405

Effects of carbon source and nitrogen concentration on the P-EPS and Chl-a production at the MMBR system  

Choi, Yun-Jeong (Department of Applied Environmental Science, Kyung Hee University)
Sim, Tae-Suk (Department of Applied Environmental Science, Kyung Hee University)
Hwang, Sun-Jin (Department of Applied Environmental Science, Kyung Hee University)
Publication Information
Journal of Korean Society of Water and Wastewater / v.35, no.6, 2021 , pp. 405-415 More about this Journal
Abstract
MMBR system has been suggested as a promising system to resolve harvesting problems induced from low settling efficiency of microalgae. And recently, a lot of research on reducing fouling at the MMBR system has investigated focused on EPS in many cases. EPS of microalgae mainly consists of polysaccharides and protein components, and is produced through photosynthesis and nitrogen-carbon metabolic pathways. Especially, P-EPS is one of major compounds which occur membrane fouling phenomenon, as its hydrophobic protein components cause floc formation and cake layer accumulation. And it is already known that almost every microalgae can metabolize P-EPS or Chl-a when nitrogen sources as a substrate is insufficient or exhausted situation. With the above backgrounds, uptake rates of P-EPS or Chl-a by Scenedesmus quadricauda according to the type of carbon source and nitrogen concentration were evaluated in order to verify correlation between carbon source vs P-EPS production, and indeed Scenedesmus quadricauda uses P-EPS or Chl-a when the amounts of nitrogen sourc es in the feed is not satisfied. As a result, it was shown that P-EPS and Chl-a production were increased proportional to nitrogen concentration under organic carbon condition. And especially, the amo unts of P-EPS and Chl-a in the cell were diminished with the nitrogen source becomes insufficient or exhausted. Because P-EPS accelerates fouling at the MMBR system, P-EPS degradation by Scenedesmus quadricauda in order to get nitrogen source may contribute to reducing fouling. About a affects of N-consumed Chl-a to the MMBR fouling, more survey is needed. On the contrary, considering the purpose of MMBR system of this study, i.e. harvesting useful high value microalgae efficiently feeding adequate industrial process wastewater, it seems like difficult to maintain satisfied metabolic activity and to harvest with high yield rate using nitrogen-poor MMBR feed.
Keywords
Microalgae; Nitrogen concentration; Protein-extracellular polymeric substances (P-EPS); Chlorophyll-a; Microalgal membrane bioreator (MMBR);
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1 Arbib, Z., Ruiz, J,. Diaz, P.A., Perez, C.G., and Perales, J.A. (2014). Capability of different microalgae species for phytoremediation processes: Wastewater tertiarytreatment, CO2 bio-fixation and low cost biofuels production, Water Res., 465-474.
2 Chen, X. Huang, C. and Liu, T. (2012). Harvesting of microalgae Scenedesmus sp. using polyvinylidene fluoride microfiltration membrane, Desalination Water Treat., 45, 177-181.   DOI
3 Da Silva, A.F., Lourenco, S.O. and Chaloub, R.M. (2009). Effects of nitrogen starvation on the photosynthetic physiology of a tropical marine microalga Rhodomonas sp., Aquat. Bot., 91(4), 291-297.   DOI
4 Dasgupta, C.N., Nayaka, S., Toppo, K., Singh, A.K., Deshpande, U. and Mohapatra, A. (2018). Draft genome sequence and detailed characterization of biofuel production by oleaginous microalga Scenedesmus quadricauda LWG002611, Biotechnol. Biofuels, 11, 308.   DOI
5 Kandimalla, P., Desi, S. and Vurimindi, H. (2016). Mixotrophic cultivation of microalgae using industrial flue gases for biodiesel production, Environ. Sci. Pollut., 23, 9345-9354.   DOI
6 Li, J., Han, D., Wang, D., Ning, K., Jia, J., Wei, L., Jing, X., Huang, S., Chen, J., Li, Y., Hu, Q., and Xu, J. (2014). Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae, Plant Cell, 26(4), 1645-1665.   DOI
7 Luo, Y. Henderson, R.K., and Le-Clech, P. (2019). Characterisation of organic matter in membrane photobioreactors (MPBRs) and its impact on membrane performance, Algal Res., 44, 101682.   DOI
8 Meng, F. Chae, S.R. Drews, A. Kraume, M. Shin, H.S. and Yang, F. (2009). Recent advances in membrane bioreactors (MBRs): membrane fouling and membrane material, Water Res., 43(6), 1489-1512.   DOI
9 Ministry of Environment. (2017). Generation and treatment of industrial wastewater, 11-1480000-001452-10.
10 Yang, C., Hua, Q., and K. Shimizu, K. (2000). Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic light-autotrophic/dark-heterotrophic conditions, Biochem. Eng. J., 6(2), 87-102.   DOI
11 Lv, J., Liu, Y., Feng, J., Liu, Q. Nan, F. and Xie, S. (2018). Nutrients removal from undiluted cattle farm wastewater by the two-stage process of microalgae-based wastewater treatment, Bioresour. Technol., 264, 311-318.   DOI
12 Da Silva Ferreira, V. and Sant'Anna, C. (2017). Impact of culture conditions on the chlorophyll content of microalgae for biotechnological applications, World J. Microbiol. Biotechnol., 33, 20.   DOI
13 Houghton, J.I., Quarmby, J. and T. Stephenson, T. (2001). Municipal wastewater sludge dewaterability and the presence of microbial extracellular polymer, Water Sci. Technol., 44(2-3), 373-9.   DOI
14 Li, Y., Horsman, M., Wang, B., Wu, N. and Lan, C.Q. (2008). Effects of nitrogen sources on cell growth and lipid accumulation of green alga neochloris oleoabundans, Appl. Microbiol. Biotechnol., 81, 629-636.   DOI
15 Babaei, A., Mehrnia, M., Shayegan, J., and Sarrafzadeh, M.H. (2018). Evaluation of nutrient removal and biomass production through mixotrophic, heterotrophic, and photoautotrophic cultivation of chlorella in nitrate and ammonium wastewater, Int. J. Environ. Res., 12, 167-178.   DOI
16 Flemming, H.C. and J. Wingender, J. (2010). The Biofilm Matrix, Nat. Rev. Microbiol., 8(9), 623-33.   DOI
17 Xiao, R. and Y. Zheng, Y. (2016). Overview of microalgal extracellular polymeric substances (EPS) and their applications, Biotechnol. Adv., 34, 1225-1244.   DOI
18 Liu, H. and Fang, H.P. (2002). Extraction of extracellular polymeric substances (EPS) of sludges, J. Biotechnol., 95(3), 249-256.   DOI