Browse > Article
http://dx.doi.org/10.11001/jksww.2016.30.6.715

Effect of high free ammonia concentration on microalgal growth and substrate uptake  

Kim, Eun-Ji (Department of Environmental Science and Engineering, Kyung Hee University)
Cho, Jae Hyung (Department of Environmental Science and Engineering, Kyung Hee University)
Noh, Kyung Ho (Department of Environmental Science and Engineering, Kyung Hee University)
Nam, guisook (Korea Rural Community Corporation)
Hwang, Sun-Jin (Department of Environmental Science and Engineering, Kyung Hee University)
Publication Information
Journal of Korean Society of Water and Wastewater / v.30, no.6, 2016 , pp. 715-723 More about this Journal
Abstract
This study investigated the effect of high concentration of free ammonia on microalgal growth and substrate removal by applying real wastewater nitrogen ratio. To test of this, the conditions of free ammonia 1, 3, 6, 9, 12, 15 mg-N/L are compared. After 3 days of incubation, algal growth of Chlorella vulgaris and carbon removal rate are respectively lower in the reactors of FA 12, 15 mg-N/L compared to the others. This indicates that the high concentration of free ammonia, in this case, above 12 mg-N/L, has negative effect on algal growth and metabolic activity. Also, high concentration of free ammonia causes the proton imbalance, ammonium accumulation in algae and has toxicity for these reasons. So, we have to consider free ammonia in applying the microalgae to wastewater treatment system by the way of diluting wastewater or controlling pH and temperature.
Keywords
Ammonium accumulation; Chlorella vulgaris; Free ammonia inhibition; Microalgae; Proton imbalance;
Citations & Related Records
연도 인용수 순위
  • Reference
1 A. Abeliovich and Y. Azov (1976) Toxicity of ammonia to algae in sewage oxidation ponds. Applied Environmental Microbiology, 31(6), 801-806
2 Baris Calli, Bulent Mertoglu, Bulent Inanc, Orhan Yenigun (2005) Effects of high free ammonia concentrations on the performances of anaerobic bioreactors. Process biochemistry, 40, 1285-1292   DOI
3 Bruce A Diner (2001) Amino acid residues involved in the coordination and assembly of the manganese cluster of photosystem II. Proton-coupled electron transport of the redox-active tyrosines and its relationship to water oxidation. Biochimica et Biophysica Acta, 1503(1-2), 147-163   DOI
4 Carmelo Rigano, Umberto Violante (1973) Effect of nitrate ammonia and nitrogen starvation on the regulation of nitrate reductase in Cyanidium caldarium. Archives of Microbiology, 90, 27-33
5 De La Noue and De Pauw (1988) The Potential of Microalgal Biotechnology: a Review of Production and Uses of Microalgae. Biotecnologia de Microalgas, 6, 725-770
6 Gallert C., Bauer S., Winter J. (1998) Effect of ammonia on the anaerobic degradation of protein by a mesophilic and thermophilic biowaste population. Applied Microbiology and Biotechnology, 50(4), 495-501   DOI
7 Greenpla (2013) The composition of digested food waste leachate into wastewater treatment plant of SUDOKWON landfill 2011-2013.
8 Hamed M. El-Mashad, Grietje Zeeman, Wilko K.P. van Loon, Gerard P.A. Bot, Gatze Lettinga (2004) Effect of temperature and temperature fluctuation on thermophilic anaerobic digestion of cattle manure. Bioresource Technology, 95(2), 191-201   DOI
9 HONG LIN and DAVID RANDALL (1991) Evidence for the presence of an electrogenic proton pump on the trout gill epithelium. Journal of experimental biology, 161, 119-13
10 JUNE M NEVIN, CAROL J LOVATT (1987) Demonstration of ammonia accumulation and toxicity in avocado leaves during water-deficit stress. South African Avocado Growers' Association Yearbook, 10, 51-54
11 Kristina N. Ferreira, Tina M. Iverson, Karim Maghlaoui, James Barber, So Iwata (2004) Architectureof the photosynthetic oxygen-evolving center. Science, 303(5665), 1831-1838   DOI
12 Miriam Drath, Nicole Kloft, Alfred Batschauer, Kay Marin, Jens Novak, and Karl Forchhammer (2008) Ammonia Triggers Photodamage of Photosystem II in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Plant Physiology, 147(1), 206-215   DOI
13 Lalucat, J., Imperial, J., Pares, R. (1984) Utilization of light for the assimilation of organic matter in Chlorella sp. VJ79. Biotechnology and Bioengineering, 26(7), 677-681.   DOI
14 L.TRAVIESO, F.BENiTEZ, E.SANCHEZ, R.BORJA, M.F.COLMENAREJO (2006) Production of Biomass (Algae-Bacteria) by Using a Mixture of Settled Swine and Sewage as Substrate. Journal of Environmental Science and Health, Part A, 41, 415-429
15 Miguel R. Aguilar, Jacobo Cardenas, Emilio Fernandez (1991) Regulation of molybdenum cofactor species in the green alga Chlamydomonas reinhardtii. Biochim Biophys Acta., 1073(3), 463-9   DOI
16 M. Losada, A. Peneque, P.J. Aparicio, J. Mvega, J. Cardenas, J. Herrera (1970) Inactivation and repression by ammonium of the nitrate reducing system in chlorella. biochemical and biophysical research communications, 38, 4, 1009-1015   DOI
17 MOAF and MOE (2004) Recycling and Management of the Livestock Manure. Ministry of Agriculture and Forestry (MOAF) and Ministry of Environment (MOE)
18 MOE(Ministry of Environment) (2012) The effluent standard of wastewater treatment plant
19 Katarzyna, C., Facundo-Joaquin, M.-R. (2004) Kinetic and stoichiometric relationships of the energy and carbon metabolism in the culture of microalgae. Biotechnology, 3(1), 21-34   DOI
20 Poggi-Varaldo, H.M., Rodr.guez-Vazquez, R., Fernandez-Villagomez, G., Esparza-Garc.a, F. (1997) Inhibition of mesophilic solidsubstrate anaerobic digestion by ammonia nitrogen. Applied microbiology and biotechnology, 47, 284-291   DOI
21 Rita B. Domingues, Ana B. Barbosa, Ulrich Sommer, Helena M. Galvao (2011) Ammonium, nitrate and phytoplankton interactions in a freshwater tidal estuarine zone : potential effects of cultural eutrophication. Aquatic Sciences, 73, 331-343   DOI
22 PRIYA C. KADAM, DAVID R. BOONE (1996) Influence of pH on Ammonia Accumulation and Toxicity in Halophilic, Methylotrophic Methanogens. American Society for Microbiology, 62(12), 4486-4492
23 Rajinikanth Rajagopal, Daniel I. Masse, Gursharan Singh (2013) A critical review on inhibition of anaerobic digestion process by excess ammonia. Bioresource Technology, 143, 632-641   DOI
24 Richard C. Dugdale, Frances P. Wilkerson, Victoria E. Hogue, Albert Marchi (2007) The role of ammonium and nitrate in spring bloom development in San Francisco Bay Estuarine, Coastal and Shelf Science, 73, 17-29   DOI
25 Wilkie AC, Mulbry WW. (2002) Recovery of dairy manure nutrients by benthic freshwater algae. Bioresource Technology, 81(1), 81-91   DOI
26 Y. Azov and JOEL C. Goldman (1982) Free Ammonia Inhibition of Algal Photosynthesis in Intensive Cultures. Applied Environmental Microbiology, 43(4), 735-739