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Enhancement of Phenanthrene Solubilization and Biodegradation with Biosurfactants Produced by Rhodococcus erythropolis  

Chang, Jae-Soo (미국 Univ. of Delaware 토목환경공학과)
Song, Changsoo (호남대학교 토목환경공학과)
Cha, Daniel K. (미국 Univ. of Delaware 토목환경공학과)
Publication Information
Journal of Korean Society of Water and Wastewater / v.18, no.6, 2004 , pp. 724-730 More about this Journal
Abstract
Effects of the biosurfactant produced by Rhodococcus erythropolis on the solubilization and biodegradation of phenanthrene were investigated. Based on surface tension measurements, the average critical micelle concentration of the biosurfactant was estimated to be about 16mg TOC/L. The apparent solubility of phenanthrene increased linearly with the addition of biosurfactants above the CMC, and the concentration of solubilized phenanthrene was 38.9mg/L in 322mg TOC/L biosurfactant solution. The weight-solubilization ratio of biosurfactants for phenanthrene was approximately 118.8mg/g, this value was over 5 times greater than that of sodium dodecyl sulfate. Using a known phenanthrene degrader, batch phenanthrene biodegradation experiments were conducted with and without biosurfactants in liquid culture. The rate and extent of phenanthrene mineralization by the phenanthrene degrader with biosurfactants were much greater than those without biosurfactants. The greater phenanthrene mineralization observed in the presence of biosurfactants is attributed to the increased phenanthrene concentration in the aqueous culture due to the partitioning of the compound to biosurfactant micelles. The biosurfactant did not exhibit any toxic effect on mineralization of glucose by the phenanthrene-degrader.
Keywords
biosurfactant; phenanthrene; solubility; biodegradation;
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