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http://dx.doi.org/10.5762/KAIS.2020.21.5.223

Growth Evaluation of Lipid Production Microalgae Scenedesmus obliquus using Raman Spectroscopy  

Yoo, Yong Jin (Department of Physics and Nano-science, Sun Moon University)
Lee, Geon Woo (Department of Physics and Nano-science, Sun Moon University)
Baek, Dong Hyun (Center for Next-Generation Semiconductor Technology, Sun Moon University)
Lee, Jin Woo (Department of Food Science, Sun Moon University)
Kim, Ho Seob (Department of Physics and Nano-science, Sun Moon University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.5, 2020 , pp. 223-229 More about this Journal
Abstract
Biodiesel produced using microorganisms, which are recognized as the third-generation biomass, is among the various known renewable energy sources that can replace fossil fuels used in conventional transportation. Scenedesmus obliquus has been identified as an excellent species for biodiesel production, as it grows faster and can accumulate up to 40-50 percent of the dry cell weight. Enhancing production using S. obliquus requires measuring the cell mass for controlling the cultivation process. In the current study, S. obliquus was cultured for 75 days, and growth changes of the microalgae were measured by absorbance, microscopic imaging, and Raman spectroscopy. Between days 60 to 75 of culture, the change in absorbance was observed to be less than 3%, whereas the number of microalgae observed microscopically was more than three times higher. Moreover, the Raman spectroscopy results showed three strong peak values of β-carotene at 997 cm-1, 1148 cm-1, and 1515 cm-1, with peak values of β-carotene showing greater than 3-fold increase during the culture period. Therefore, we predict that application of Raman spectroscopy will help in identifying the growth elements and growth degree in microalgae culture during increased biomass production.
Keywords
Microalgae; Scenedesmus obliquus; LED light; Incubation time; Raman spectrum;
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Times Cited By KSCI : 4  (Citation Analysis)
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