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http://dx.doi.org/10.5762/KAIS.2020.21.5.216

Characterization of TRAF4 mRNA and Functions related to tight junction in pig  

Yun, Jeong-hee (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration)
Hwang, In-Sul (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration)
Hwang, Seongsoo (Division for KOPIA, Rural Development Administration)
Park, Mi-Ryung (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.5, 2020 , pp. 216-222 More about this Journal
Abstract
Tumor necrosis factor receptor associated factor 4 (TRAF4) is found to be overexpressed in human breast cancer. It plays a role in cancer metastasis, production of reactive oxygen species, and cell polarity at membranes. The characteristics and functions of TRAF4 in pigs have not yet been identified. As the first step of research, the mRNA sequence of TRAF4 in porcine cells has been determined. To obtain the full-length sequence, rapid amplification of cDNA ends (RACE) has been carried out. Upon cloning, 2,030 bp of nucleotides were found to encode 470 amino acids, and 8 and 12 amino acids were different from those of the human and mouse TRAF4, respectively. The coding region of porcine TRAF4 was shown to be 93% and 90% homologous to human and mouse TRAF4, respectively. qPCR was conducted to determine the relative expression level of TRAF4. TRAF4 expression in pK15 was enhanced by cell-cell contacts. The mRNA levels of CLDN4, OCLN, and TJP1 at 60% and 80% confluency were significantly higher than at 40% confluency. Further, TRAF4 and tight junction-related genes were down-regulated upon treatment with TRAF4 siRNA. Thus, TRAF4 may affect the function of tight junctions in pig.
Keywords
Pig; TRAF4; Tight junction; PK15; Sequence;
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