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http://dx.doi.org/10.5762/KAIS.2020.21.3.199

Effects of Mitochondria-targeted Antioxidant MitoTEMPO on the Kinetic Characteristics of Frozen-Thawed Boar Sperm  

Cho, Eun Seok (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Kim, Jeong A (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Jeong, Yong Dae (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Choi, Yo Han (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Hong, Jun Ki (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Kim, Young Sin (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Chung, Hak Jae (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Baek, Sun Young (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Sa, Soo Jin (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.3, 2020 , pp. 199-205 More about this Journal
Abstract
Cryopreservation of semen is useful for animal breeding via artificial insemination (AI). However, the use of frozen-thawed boar semen is limited due to cryodamage. The aim of this study was to investigate the effects of different concentrations of MitoTEMPO (a mitochondria-targeted antioxidant) in lactose-egg yolk (LEY) extenders on kinetic characteristics of frozen-thawed boar sperms. Semen samples were collected from mature Duroc boars (2~3 years old) and cryopreserved in LEY extenders containing 0, 0.5, 5, 50, and 500 μM MitoTEMPO. The kinetic characteristics of frozen-thawed sperms were determined 0 and 30 min after thawing using computer-assisted sperm analysis (CASA). Results indicated that sperm motility immediately after thawing was significantly higher with 5 and 50 μM (50.46±2.71% and 46.96±2.66%, respectively) than with 500 μM MitoTEMPO (35.40±2.95%) (P<0.05). However, there were no significant differences in other kinetic characteristics except motility. In conclusion, the addition of MitoTEMPO to the sperm freezing extender may have a beneficial effect on motility of post-thawed boar semen.
Keywords
Boar; Cryopreservation; Kinetic characteristics; MitoTEMPO; Spermatozoa;
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