Browse > Article
http://dx.doi.org/10.5762/KAIS.2020.21.2.432

Research fecal metabolite according to fed different ratios of roughage to concentrate on lactating cow using 1H-NMR analysis  

Kim, Hyun Sang (Division of Applied Life Science(BK21Plus), Gyeongsang National University)
Lee, Shin Ja (Institue of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University)
Eom, Jun Sik (Division of Applied Life Science(BK21Plus), Gyeongsang National University)
Lee, Sung Sill (Division of Applied Life Science(BK21Plus), Gyeongsang National University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.2, 2020 , pp. 432-439 More about this Journal
Abstract
This study examined the metabolites in different roughage to concentrate ratios using proton nuclear magnetic resonance spectroscopy (1H-NMR). Six lactating cows were divided into two groups that were fed different roughage to concentrate ratios (HR group = 8:2, HC group = 2:8). Feces samples were collected individually at one time, and the metabolites were analyzed using an SPE-800 MHz NMR-MS system. The metabolites were identified and quantified using a Chenomx NMR suite 8.4. Metabolic pathway analysis and principal component analysis were conducted using a Metaboanalyst 4.0. Statistical analysis was performed using a Dunnett's test on the SAS program. As a result, several metabolites were identified, and among them, 77 metabolites were used in statistical analysis. The levels of twelve metabolites were significantly higher in the HC group: succinate, dimethylamine, histamine, homovanillate, thymol, acetate, propionate, butyrate, isovalerate, valerate, imidazole, N-nitrosodimethylamine, and O-acetylcholine. In the HC group, the concentrations of all metabolites were higher than in the HR group, and the metabolic pathway was also different. This study is expected to be useful for a variety of livestock studies by 1H-NMR because it examined the change in metabolites in the body metabolism and microorganisms.
Keywords
Dairy Cow; Feces; Feeding Ratio; Metabolite; NMR;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 J. S. Eom, S. J. Lee, S. K. Lee, Y. J. Lee, H. S. Kim, Y. Y. Choi, K. S. Ki, H. Y. Jeong, E. T. Kim, S. S. Lee, C. D. Lee, S. S. Lee. "Effects of different roughage to concentrate ratios on the changes of productivity and metabolic profiles in milk of dairy cows", Korean Journal of Organic Agriculture, Vol.27, No.2, pp 147-160. May 2019. DOI: https://dx.doi.org/10.11625/KJOA.2019.27.2.147   DOI
2 O. Deda, A. C. Chatziioannou, S. Fasoula, D. Palachanis, N. Raikos, G. A. Theodoridis, H. G. Gika, "Sample preparation optimization in fecal metabolic profiling", Journal of Chromatography B, Vol.1047, No.15, pp.115-123, Mar. 2017. DOI: https://doi.org/10.1016/j.jchromb.2016.06.047
3 B. K. Kim, C. B. Choi, S. O. Lee, K. H. Baek, D. J. Jung, E. G. Hwang "Effects of supplementing herbs on growth performances, blood composition and diarrhea in Hanwoo calves", Journal of Animal Science and Technology, Vol.53, No.5, pp.451-459, Oct. 2011. DOI: https://doi.org/10.5187/JAST.2011.53.5.451
4 C. W. Dunnett. "A multiple comparison procedure for comparing several treatments with a control", Journal of the American Statistical Association, Vol.50 No.272, pp.1096-1121. Apr. 1955 DOI: https://doi.org/10.1080/01621459.1955.10501294   DOI
5 M. T. Yokoyama, J. R. Carlson, "Production of Skatole and para-Cresol by a Rumen Lactobacillus sp.", Applied and Enviromental Microbiology, Vol.41, No.1, pp.71-76, Jan. 1981. DOI: https://doi.org/10.1128/aem.41.1.71-76.1981
6 F. Saleem, B. N. Ametaj, S. Bouatra, R. Mandal, Q. Zebeli, S. M. Dunn, D. S. Wishart, "A metabolomics approach to uncover the effects of grain diets on rumen health in dairy cows", Journal of Dairy Science, Vol.95, No.11, pp.6604-6623, Nov. 2012. DOI: https://doi.org/10.3168/jds.2012-5403
7 M. Friedman, "Chemistry and multibeneficial bioactivities of carvacrol (4-isopropyl-2-methylphenol), a component of essential oils produced by aromatic plants and spices", Journal of agricultural and food chemistry, Vol.62, No.31, pp.7652-7670, Jul. 2014. DOI : https://doi.org/10.1021/jf5023862   DOI
8 S. Mao, R. Zhang, D. Wang, W. Zhu, "The diversity of the fecal bacterial community and its relationship with the concentration of volatile fatty acids in the feces during subacute rumen acidosis in dairy cows", BMC Veterinary Research, Vol.8, pp.237, Dec. 2012. DOI: http://www.biomedcentral.com/1746-6148/8/237
9 A. Sivropoulou, E. Papanikolaou, C. Nikolaou, S. Kokkini, T. Lanaras, M. Arsenakis, "Antimicrobial and cytotoxic activities of Origanum essential oils", Journal of Agricultural and Food Chemistry, Vol.44, No.5. pp.1202-1205, May. 1996. DOI: https://doi.org/10.1021/jf950540t   DOI
10 Y. J. Chen, O. S. Kwon, B. J. Kim, K. S. Shon, J. H. Cho, I. H. Kim, "The effects of dietary biotite V supplementation on growth performance, nutrients digestibility and fecal noxious gas content in finishing pigs", Asian-Australasian Journal of Animal Science, Vol.18, No.8, pp.1147-1152, Dec. 2005. DOI: https://doi.org/10.5713/ajas.2005.1147   DOI
11 D. N. Miller, V. H. Varel, "In vitro study of the biochemical origin and production limits of odorous compounds in cattle feedlots", Journal of Animal Science, Vol.79, No.12, pp.2949-2956, Dec. 2001. DOI: https://doi.org/10.2527/2001.79122949x   DOI
12 D. N. Miller, V. H. Varel, "Swine manure composition affects the biochemical origins, composition, and accumulation of odorous compounds", Journal of Animal Science, Vol.81, No.9, pp.2131-2138, Sep. 2003. DOI: https://doi.org/10.2527/2003.8192131x   DOI
13 S. H. Kim, S. O. Yang, K. H. Kim, Y. S. Kim, K. H. Liu, Y. R. Yoon, D. Lee, C. H. Lee, G. S. Hwang, M. W. Chung, K. H. Choi, H. K. Choi. "Research trends, applications, and domestic research promotion stratigies of metabolomics", The Korean Society for Biotechnology and Bioengineering, Vol.24, No.2, pp.113-121, Jun. 2009. DOI: https://www.earticle.net/Article/A110997
14 D. H. Kim, I. B. Lee, D. Y. Choi, J. I. Song, J. H. Jeon, D. M. Ha. "A Survey on Current State of Odor Emission and Control from Livestock Operations", Journal of Animal Environmental Science, Vol.19, No.2, pp.123-132, Dec. 2013. DOI: https://doi.org/10.11109/JAES.2013.19.2.123   DOI
15 C. W. Song, N. C. Kim, J. K. Ryu, J. M. Kim. "A study on the fuelization of livestock sludge using thermal hydrolysis", Journal of Korea Organic Resource Recycling Association, Vol.23, No.3, pp.51-59, Sep. 2015. DOI: https://doi.org/10.17137/korrae.2015.23.3.051   DOI
16 P. D. Le, A. J. A. Aarnink, N. W. M. Ogink, P. M. Becker, M. W. A. Verstegen. "Odour from animal production facilities: its relationship to diet", Nutrition Research Reviews, Vol.18, No.1, pp.3-30, Jun. 2005. DOI: https://doi.org/10.1079/NRR200592   DOI
17 S. H. Seo, S. E. Park, E. J. Kim, D H. Youn, Y. M. Lee, S. Y. Lee, S. H. Bok, D. H. Park, C. S. Seo, S. H. Byun, K. Y. Jun, D. S. Kim, C. S. Na, H. S. Son, "GC/MS-based metabolomics approach to evaluate the effect of jackyakgamcho-tang on acute colitis", Evidence-Based Complementary and Alternative Medicine, Vol.2019, Article ID 4572764, Jan. 2019. DOI: https://doi.org/10.1155/2019/4572764
18 Y. Mu, X. Lin, Z. Wang, Q. Hou, Y. Wang Z. Hu, "Highproduction dairy cattle exhibit different rumen and fecal bacterial community and rumen metabolite profile than low-production cattle", MicrobiologyOpen, Vol.8, No.4, pp.e00673, Apr. 2019. DOI: https://doi.org/10.1002/mbo3.673
19 N. Sillner, A. Walker, D. Hemmler, M. Bazanella, S. S. Heinzmann, D. Haller, P. Schmitt-Kopplin, "Milk-derived amadori products in feces of formula-fed infants", Agricultural and Food Chemistry, Vol.67, No.28 pp.8061-8069, Jun. 2019. DOI: https://doi.org/10.1021/acs.jafc.9b01889
20 J. Fiori, S. Turroni, M. Candela, R. Gotti, "Assessment of gut microbiota fecal metabolites by chromatographic targeted approaches (Review)", Journal of Pharmaceutical and Biomedical Analysis, Vol.177, No.2020, pp.112867, Sep. 2019. DOI: https://doi.org/10.1016/j.jpba.2019.112867
21 J. S. Eom, S. J. Lee, Y. G. Lee, S. S. Lee. "Comparison of volatile fatty acids, monosaccharide analysis and metabolic profiling in rumen fluid according to feeding methods", Journal of the Korea Academia-Industrial cooperation Society, Vol. 19, No. 12, pp 814-824. Dec. 2018. DOI: https://doi.org/10.5762/KAIS.2018.19.12.814   DOI
22 K. Y. Park, M. C. Kim, K. L. Woo, N. K. Lee, H. D. Paik, "Rapid analysis of major putrefactive metabolites by GC and GC/MSD", Korean Society for Biotechnology and Bioengineering Journal, Vol.18, No.1, pp.74-77, Feb 2003. DOI: https://www.earticle.net/Article/A101219