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http://dx.doi.org/10.5762/KAIS.2020.21.10.433

Embryo Production from Elk using Ultrasound-Guided Ovum Pick-Up Technique  

Lee, Eun-Do (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Dong-Kyo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.10, 2020 , pp. 433-439 More about this Journal
Abstract
This study, which was designed to improve and increase the utilization of female elk, examined the possibility of collecting their embryos during the non-reproductive period using ultrasound-guided Ovum Pick-Up (OPU) technology. Once follicles in the ovaries of an elk were observed, the embryos were retrieved through the absorption of the follicles using an OPU ultrasound probe. Fifty-seven embryos were retrieved from 85 follicles, giving a retrieval rate of 67.1%. Morphological evaluation of the recovered embryos revealed 14.0% embryos to be in grade A, 19.2% in grade B, 15.7% in grade C, and 50.8% in grade D. The developmental efficiency of the retrieved embryos was also investigated using in vitro maturation, in vitro fertilization, and in vitro culture. After cultivating 28 embryos through in vitro fertilization, 19 embryos were found to be fertilized; the fertilization rate was 67.9%. Four embryos developed to the blastocyst stage, indicating a 14.3% development rate. This study confirmed that the production of fertilized embryos from a seasonal breeder, such as elk, is possible via the ultrasound-guided OPU method. If the efficiency of in vitro fertilization and in vitro culture can be improved through further research, it will help improve the efficiency of elk embryo production through the transplantation of their fertilized embryos.
Keywords
Elk; Deer; In vitro Culture; Ovum Pick-Up; Embryo;
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