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http://dx.doi.org/10.5762/KAIS.2019.20.12.298

Cudrania Tricuspidata root extract (CTE) has an anti-platelet effect via cGMP-dependent VASP phosphorylation in human platelets  

Ro, Ju-Ye (Department of Biomedical Laboratory Science, Konyang University)
Cho, Hyun-Jeong (Department of Biomedical Laboratory Science, Konyang University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.20, no.12, 2019 , pp. 298-305 More about this Journal
Abstract
Cudrania tricuspidata has been reported to have many biological activities, including anti-inflammatory, anti-cancer, and antioxidant properties. However, the effects of C. tricuspidata root extract (CTE) on human platelet aggregation induced by collagen as well as the signaling pathways involved remain unknown. In the present study, we investigated the effect of CTE on human platelets. CTE inhibited platelet aggregation via down-regulation of thromboxane A2 (TXA2) by blocking cyclooxygenase-1 (COX-1) activity and intracellular Ca2+ mobilization in collagen-induced platelets. CTE also reduced the phosphorylation of phospholipase C (PLC) γ2 and syk. CTE regulated platelet aggregation via cyclic guanosine monophosphate (cGMP)-dependent phosphorylation of vasodilator-stimulated phosphoprotein (VASP) Ser239. In addition, administration of CTE (50 and 100 mg/kg) significantly reduced hyper-aggregated platelet aggregation by collagen (5 ㎍/mL) without hepatotoxicity in HFD (high fat diet)-fed rats. Taken together, these results suggest that CTE has anti-platelet effects both in vitro and in vivo. Therefore, CTE may be an effective therapeutic and preventive agent for cardiovascular disease, and is a safe and natural product.
Keywords
Cudrania Tricuspidata; Cyclic Guanosine Monophosphate; Cyclooxygenase-1 Activity; Platelet Aggregation; Phospholipase $C{\gamma}2$; Syk; Thromboxane $A_2$; Vasodilator-stimulated Phosphoprotein;
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