Browse > Article
http://dx.doi.org/10.5762/KAIS.2017.18.12.503

The anti-inflammation effects of A.C.C. extracts on the LPS-induced Raw 264.7 cell  

Ryu, Jin-Hyeob (Nature4 Co. Ltd)
An, Ju-Hee (Nature4 Co. Ltd)
Woo, Yong-Kyu (Nature4 Co. Ltd)
Cho, Hyun-Jeong (Department of Biomedical Laboratory Science, College of Medical Science, Konyang University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.18, no.12, 2017 , pp. 503-511 More about this Journal
Abstract
This study was conducted to evaluate the anti-inflammatory activity and clinical efficacy of a sample (A.C.C. extracts) obtained by distillation extraction of 14 herbal medicines including Phellodendron bark. To confirm this, the amount of nitric oxide (NO) produced by the cells in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) and the changes in the production of inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$, interleukin(IL)-$1{\beta}$ and IL-6 were determined. The results showed that A.C.C. extracts strongly inhibited the production of NO and inflammatory cytokines increased by LPS without cytotoxicity. In addition, A.C.C. extracts showed strong bacterial reduction rates of 99.9% in Pseudomonas aeruginosa, Staphylococcus aureus, MRSA (Methicillin-resistant Staphylococcus aureus), Candida albicans and Streptococcus mutans. These findings indicate that A.C.C. extracts are effective ingredients with a strong antimicrobial effect together with an anti-inflammatory effect. In addition, when A.C.C. extracts were applied to infants and toddlers who were suffering from diaper rash, itching, and perspiration symptoms, symptoms of rash, atopy, rash, itching, and heat rash were improved. After the lapse of time, it was visually confirmed that it was considerably relaxed. These findings confirm that A.C.C. extracts comprise a clinically effective anti-inflammatory and anti-bacterial agent that alleviates symptoms such as diaper rash and fever and may therefore be an effective alternative to inflammatory diseases.
Keywords
A.C.C. extracts; Anti-bacterial; Anti-inflammation; Cytokine; Lipopolysaccharide-induced Raw 264.7 cell; Nitric oxide;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 J. H. Moon, H. Go, S. M. Shin, K. T. Kim, "Anti-inflammatory effect of extracts from Ligustrum obtusifolium S. fruits in RAW 264.7 macrophages", J. Soc. Korean Med. Diagn, vol. 17, pp. 263-273, 2013.
2 S. L. Masters, A. Simon, I. Aksentijevich, D. L. Kastner, "Horror Auto inflammaticus: The Molecular Pathophysiology of Autoinflammatory Disease", Annual Review of Immunology, vol. 27, no. 1, pp. 621-668, 2009. DOI: https://doi.org/10.1146/annurev.immunol.25.022106.141627   DOI
3 D. H. Kim, S. J. Park, J. Y. Jung, S. C. Kim, S. H. Byun, "Anti-inflammatory Effects of the Aqueous Extract of Hwangnyeonhaedok-tang in LPS-activated Macrophage Cells", Korean J. Herbol, vol. 24, no. 4, pp. 39-47, 2009.
4 M. L. McDaniel, G. Kwon, J. R. Hill, C. A. Marshall, J. A. Corbett, "Cytokines and nitric oxides in islet inflammation and diabetes", Proc. Soc. Exp. Biol. Med, vol. 211, no. 1, pp. 24-32, January, 1996. DOI: https://doi.org/10.3181/00379727-211-43950D   DOI
5 K. Min-Ji, B. Nan-Yong, K. Koth-Bong-Woo-Ri, P. Ji-Hye, P. Sun-Hee, J. Mi-Ran, A. Dong-Hyun, "Anti-Inflammatory Effect of Chondrus nipponicus Yendo Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells", Journal of the Korean Society of Food Science and Nutrition, vol. 45, no. 2, pp. 194-201, February, 2016. DOI: https://doi.org/10.3746/jkfn.2016.45.2.194   DOI
6 T. H. Lee, H. B. Kwak, H. H. Kim, Z. H. Lee, D. K. Chung, N. I. Baek, J. Kim, "Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-${\kappa}B$ Transactivation in LPS-activated RAW 264.7 Cells", Mol. Cells, vol. 23, no. 3, pp. 398-404, 2007.
7 J. MacMicking, A Qiaowen Xie, C. Nathan, "NITRIC OXIDE AND MACROPHAGE FUNCTION", Annual Review of Immunology, vol. 15, no. 1, pp. 323-350, 1997. DOI: https://doi.org/10.1146/annurev.immunol.15.1.323   DOI
8 S. G. Park, K. H. Jegal, J. Y. Jung, Y. D. Back, S. H. Byun, Y. W. Kim, "Leonuri Fructus Ameliorates Acute Inflammation via the Inhibition of NF-${\kappa}B$-mediated Nitric Oxide and Pro-inflammatory Cytokine Production", Journal of Physiology & Pathology in Korean Medicine, vol. 28, no. 2, pp. 178-185, March, 2014. DOI: https://doi.org/10.15188/kjopp.2014.04.28.2.178
9 B. B. Lee, Y. B. Chae, Y. K. Kwon, C. H. Yang, M. R. Kim, K. J. Kim, D. H. Hahm, H. J. Lee, "Inhibitory Action of Cortex Phellodendris on Nicotine-induced Behavioral Sensitization", Korean J. Physiology & Pathology, vol. 18, no. 3, pp. 767-773. 2004
10 Y. Y. Lim, H. M. Kim, W. S. Park, J. H. Kim, H. J. Shin, M. N. Kim, B. J. Kim, "Anti-inflammatory and Anti-pruritic Effects of Portulaca oleracea L. Extract Using In Vitro and In Vivo Inflammation Model: LPS-treated Raw264.7 Cells, Keratinocytes, NC/Nga Mice and Hairless SKH-1 Mice", The Korean Asthma and Allergy, vol. 31, no. 3, pp. 199-206, 2011.
11 S. Singh, A. Kumar, A. Agrawal, R. Singh, "Study of Dermatophytes and incidence of different clinical types of Tinea in skin OPD", Eastern Journal of Medical Sciences, vol. 1, no. 1, pp. 24-30, 2016.
12 J. McCord, K. Wong, S. Stokes, W. Petrone, D. English, "Superoxide and inflammation: a mechanism for the anti-inflammatory activity of superoxide dismutase", Acta Physiol. Scand. Suppl, vol. 492, pp. 25-30, January, 1980.
13 N. Azad, Y. Rojanasakul, V. Vallyathan, "Inflammation and lung cancer: roles of reactive oxygen/nitrogen species", J. Toxicol. Environ. Health B, vol. 11, no. 1, pp. 1-15, January, 2008. DOI: https://doi.org/10.1080/10937400701436460   DOI
14 S.-G. Lee, M.-M. Kim, "Anti-inflammatory effect of scopoletin in RAW264.7 macrophages", Journal of Life Science, vol. 25, no. 12, pp. 1377-1383, October, 2015. DOI: https://doi.org/10.5352/JLS.2015.25.12.1377   DOI
15 J. Y. Kim, K. S. Jung, H. G. Jeong, "Suppressive effects of the kahweol and cafestol on cyclooxygenase‐2 expression in macrophages", FEBS letters, vol. 569, no. 1-3, pp. 321-326, June, 2004. DOI: https://doi.org/10.1016/j.febslet.2004.05.070   DOI
16 S. T. Lee, Y. R. Jeong, M. H. Ha, S. H. Kim, M. W. Byun, S. K. Jo," Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extract in mouse macrophage", J. Korean Soc. Food Sci. Nutr, vol. 29, pp. 342-348, 2000.
17 M. M. Mu, D. Chakravortty, T. Sugiyama, N. Koide, K. Takahashi, I. Mori, T. Yoshida, T. Yokochi, "The inhibitory action of quercetin on lipopolysaccharideinduced nitric oxide production in RAW 264.7 macrophage cells", J Endotoxin Res, vol. 7, no. 6, pp. 431-438, December, 2001. DOI: https://doi.org/10.1179/096805101101533034   DOI
18 J. H. Ryu, H. Ahn, J. Y. Kim, Y. K. Kim, "Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages", Phytother Res, vol. 17, no. 5, pp. 485-489, May, 2003. DOI: https://doi.org/10.1002/ptr.1180   DOI
19 B. Halliwell, J. Gutteridge, "Oxygen toxicity, oxygen radicals, transition metals and disease", Biochemical Journal, vol. 219, no. 1, pp. 1-14, April, 1984. DOI: https://doi.org/10.1042/bj2190001   DOI
20 E. Hyun, H. Lee, W. Yoon, S. Park, H. Kang, S. Kim, E. Yoo, "Inhibitory effect of Salvia officinalis on the inflammatory cytokines and inducible nitric oxide synthesis in murine macrophage RAW264. 7", Yakhak Hoeji, vol. 48, pp. 159-164, 2004.
21 J. W. Lee, H. W. Ryu, S. Y. Park, H. A. Park, O. K. Kwon, H. J. Yuk, K. K. Shrestha, M. Park, J. H. Kim, S. Lee, S. R. Oh, K. S. Ahn, "Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation", Int J Mol Med. vol. 40, no. 6, pp. 1932-1940, December, 2017. DOI: https://doi.org/10.3892/ijmm.2017.3178
22 T. Ljung, S. Lundberg, M. Varsanyi, C. Ohansson, P. T. Schmidt, M. Herulf, "Rectal nitric oxide as biomaker in the treatment of inflammatory bowel disease: responders versus non-responders", World Gastroenterol, vol. 12, no. 21, pp. 3386-3392, June, 2006. DOI: https://doi.org/10.3748/wjg.v12.i21.3386   DOI
23 J. B. Jeong, S. C. Hong, H. J. Jeong, J. S. Koo, "Anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino on LPS-stimulated RAW 264.7 and THP-1 cells", Korean Journal of Plant Resources, vol. 25, no. 3, pp. 299-307, 2012. DOI: https://doi.org/10.7732/kjpr.2012.25.3.299   DOI
24 J. Frostegard, A. K. Ulfgren, P. Nyberg, U. Hedin, J. Swedenborg, U. Andersson, G. K. Hansson, "Cytokine expression in advanced human atherosclerotic plaques: dominance of pro-inflammatory (Th1) and macrophage-stimulating cytokines", Atherosclerosis, vol. 145, no. 1, pp. 33-43, July, 1999. DOI: https://doi.org/10.1016/S0021-9150(99)00011-8   DOI
25 J. Shi, S. Shan, H. Li, G. Song, Z. Li, "Anti-inflammatory effects of millet bran derived-bound polyphenols in LPS-induced HT-29 cell via ROS/miR-149/Akt/NF-${\kappa}B$ signaling pathway", Oncotarget, vol. 8, no. 43, pp. 74582-74594, September, 2017. DOI: https://doi.org/10.18632/oncotarget.20216
26 H. Zhang, B. Zhang, X. Zhang, X. Wang, K. Wu, Q. Guan, "Effects of cathelicidin-derived peptide from reptiles on lipopolysaccharide-induced intestinal inflammation in weaned piglets", Vet Immunol Immunopathol, vol. 192, pp. 41-53, October, 2017. DOI: https://doi.org/10.1016/j.vetimm.2017.09.005   DOI