Browse > Article
http://dx.doi.org/10.5762/KAIS.2016.17.10.432

Effects of Lespedeza Caneata ethanol extract on the liver of alcohol administered Mice  

Cheong, Min-Ju (Dept. of life science, Chosun University)
Chung, Kyoung-A (Dept. of Occupational Therapy, Dongshin University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.17, no.10, 2016 , pp. 432-438 More about this Journal
Abstract
This study was conducted to investigate the effects of Lespedeza caneata extract on the livers of alcohol-administered mice. The study subjects were divided into a control (Con), alcohol administration (AL), alcohol and Lespedeza Caneata extract 200 mg/kg administration (AL-LC 200), and alcohol and Lespedeza caneata extract400 mg/kg administration (AL-LC 400) group. Distilled water was administrated orally to control and alcohol groups for ten days, while Lespedeza caneata extract was administered orally to alcohol and Lespedeza caneata extract groups for ten days. All experimental groups were fasted for twelve hours seven days after the oral administration, after which distilled water was administered orally to Con five times at twelve-hour intervals. At the same time, 50% ethanol (MERCK, USA) at 10 g/kg concentration was administered orally to AL and AL-LC groups five times at 12-hour intervals. The AST, ALT enzyme activation in blood and histology of the liver were then evaluated. AST and ALT in AL-LC groups were lower than in the AL group. Particularly, the AL-LC 200 and AL-LC 400 groups had significantly lower AST activation than the AL group. Histological results showed that most of the subjects in the AL group had necrosis and deformation in their livers, while fat droplets were accumulated in hepatic cells around the central vein. AL-LC 200 group revealed that a portion of the central vein was swollen, liver cells were expanded, and small fat droplets were accumulated. In the AL-CL 400 group, the central vein was normal and small fat droplets were accumulated in some liver cells. However, most of the liver cells appeared normal in the AL-CL 400 group. These results suggest that the extracts of Lespedeza caneata prevented alcohol induced liver damage in mice and have great potential for use as natural health products.
Keywords
ALT; AST; alcohol; Lespedeza Caneata extract; liver;
Citations & Related Records
Times Cited By KSCI : 20  (Citation Analysis)
연도 인용수 순위
1 H. Y. Yu, C. M. Kim, K. H. Koo, "An experimental study on the effect of ginseng saponin upon alcoholic liver injury", J. Hanyang Med. Coll. 2(2), pp. 287-304, 1982.
2 Segye Times 3. 17. 2016.
3 M. J. Kim, J. Jeon, S. P. Lee, J. S. Lee, "Protective Effects of Fucoidan against Acute Alcohol-induced Liver Injury in Rats" kor. J. Food Sci. Technol. 46(2), pp. 219-223, 2014. DOI: http://dx.doi.org/10.9721/KJFST.2014.46.2.219   DOI
4 A. Szilagyi, "Liver diseases in the alcoholic." Can. Fam. Physician. 32, pp. 1938-1941, 1986.
5 E. H. Lee, J. H. Chyun, "Effects of beta-carotene supplementation on lipid peroxide levels and antioxidative enzyme activities in alcoholic fatty liver rats" Korean J. Nutr. 38, pp. 289-296, 2005.
6 D. H. Kim, H. K. Kim "A study on pruritus of female alcoholics" J. Kor. Aca. Indus. coope. Soc. 14(9), pp. 4412-4420, 2013.   DOI
7 Y. T. Chung, K. A. Chung, S. S. Chang, "Human Physiology" Cheong Gu moonhwasa, Seoul. pp. 184-186 2009.
8 D. H. Youn, W. I. Kim, C. S. Na, "Effects of combination pear extract with Daekumeumjagami medication on hepatic injury induced by alcohol in mice" Kor. J. Herbology 30(1), pp. 51-57, 2015. DOI: http://dx.doi.org/10.6116/kjh.2015.30.1.51.   DOI
9 W. S. Choi, D. Y. Jang, K. M. Cha, C. K. Park "Enhanced Activities of Alcohol Methabolism by Extracts from Hericium erinaceum Hypha Cultivated with Artermisia capillaris(HEAC) " j . Kor. Aca. Indus. coope. Soc. 9(1), pp. 189-194, 2013.   DOI
10 H. K. An, J. Y. Park, H. J. Yoon "Association of among alcohol consumption and blood vitamin D and lead concentrations: Basedon 2010-2012 Korea national health and nutrition examination survey" JKAIS, 16(1) pp. 498-506, 2015. DOI: http://dx.doi.org/10.5762/KAIS.2015.16.1.498
11 B. S. Cho, J. J. Lee, M. Y. Lee "Effects of Ethanol Extracts from Petasites japonicus S. et Z. Max. on Hepatic Antioxidative Systems in Alcohol Treated Rats" J Korean Soc Food Sci. Nutr. 36(3), pp. 298-304, 2007. DOI: http://dx.doi.org/10.3746/jkfn.2007.36.3.298   DOI
12 S. K. Park, Y. C. Cho, "Relationships Between Alcohol Intake and Blood Pressure, Serum Lipids, Liver Function Tests and ObesityIndices in Middle-Aged Male Workers" JKAIS, 16,(5), pp. 3323-3332, 2015. DOI: http://dx.doi.org/10.5762/KAIS.2015.16.5.3323
13 G. H. Choi, J. G. Kim, S. T. Kwon, "Protective Effects of Food Including Hovenia dulcis on Acute Alcohol Intoxication" J Korean Soc Food Sci Nutr 40(8), pp. 1107-1112, 2011. DOI: http://dx.doi.org/10.3746/jkfn.2011.40.8.1107   DOI
14 S. H. Lee, Y. S. Song, S. Y. Lee, S. Y. Kim, K. S. Ko "Protective Effects of Akebia quinata Fruit Extract on Acute Alcohol-induced Hepatotoxicity in Mice" J. Food Sci. Techol. 46(5), pp. 622-629, 2014. DOI: http://dx.doi.org/10.9721/kjfst.2014.46.5.622
15 Y. J. Choi, K. I. Jung "Anti-Diabetic, Alcohol-Metabolizing, and Hepatoprotective Activities of Moringa (Moringa oleifera Lam.) Leaf Extracts" J Kor. Soc Food Sci. Nutr. 45(6), pp. 819-827, 2016.   DOI
16 C. K. Han, K. S. Seong, K.W. Lee, S. S. Park, J. Y. Jeong, S. S. Kim "Effects of Omija (Schizandra chinensis Baillon) Concentrate on BloodAlcohol Clearance and Hepatoprotective Function in Rats Induced by Acute Ethanol Intoxication and Chronic Ethanol Treatment" J. Kor. Soc. Food Sci. Nutr. 43(8), pp. 1139-1147, 2014. DOI: http://dx.doi.org/10.3746/jkfn.2014.43.8.1139   DOI
17 A. Numata, K. Hokimoto, H. Yamaguchi, "C-Glycosylflavones in Lespedeza cuneata", Chem. Pharm. Bull., 28, pp. 964-965, 1980. DOI: http://dx.doi.org/10.1248/cpb.28.964   DOI
18 E. K. Cho, Kyung Im Jung, Young Ju Choi Anti-Diabetic, Alcohol Metabolizing Enzyme, and Hepatoprotective Activity of Acer tegmentosum Maxim. Stem Extract" J. Kor. Soc. Food Sci. Nutr. 44(12), pp. 1785-1792, 2015. DOI: http://dx.doi.org/10.3746/jkfn.2015.44.12.1785   DOI
19 H. J. Kim, K. S. Kim, D. I. Kim, "Inhibitory effects of Lespedeza cuneata ethanol extract on ultraviolet-induced photo aging" J Korean Soc Food Sci Nutr 41, pp. 1540-1545, 2012. DOI: http://dx.doi.org/10.3746/jkfn.2012.41.11.1540   DOI
20 H. M. Park, J. H. Hong, "Physiological activities of Lespedeza cuneata extracts" Korean J. Food Preserv. 21(6), pp. 844-850, 2014. DOI: http://dx.doi.org/10.11002/kjfp.2014.21.6.844   DOI
21 S. Matsuura, M. Iinuma, E. Ito, H. Takami, K. Kagei, "Studies on the constituents of the useful plants. VIII. The constituents of Lespedeza cuneata G. Don." Yakugaku Zasshi 98, pp. 1542-1544, 1978.   DOI
22 H. J. Lee, G. N. Lim, M. A. Park, S. N. Park, "Antibacterial and antioxidative activity of Lespedeza cuneata G. Don." Kor. J. Microbiol Biotechnol 39, pp. 63-69, 2011.
23 H. K. Jung, K. S. Kim, Y. S. Jeong, "Wound Healing Effects of Lespedeza cuneata Extract" J. Kor. Soc. Food Sci. Nutr, 43(3), pp. 374-380, 2014. DOI: http://dx.doi.org/10.3746/jkfn.2014.43.3.374   DOI
24 H. I. Lee, J Y. Jung, M. B. Hwang, S. K. Ku, Y. W. Kim, S. Y. Jee, "Anti-inflammatory effects of Lespedeza Cuneata in vivo and in vitro", Kor J Herbology, 28(4), pp. 83-92, 2013. DOI: http://dx.doi.org/10.6116/kjh.2013.28.4.83
25 S. Y. Joo "Preventive medicine" Gaechuk moonhwasa, Seoul. pp. 288-294 1995.
26 E. J. Cho, H. M. Ju, C. H. Jeong, S. H. Eom, H. J. Heo, D. O. Kim, "Effect of Phenolic Extract of Dry Leaves of Lespedeza cuneata G. Don on Antioxidant Capacity and Tyrosinase Inhibition", Kor. J. Hort. Sci. Technol. 29(4), pp. 358-365, 2011.
27 D. I. Kim, J. H. Hong, "Optimization of Ethanol Extraction Conditions for Functional Components from Lespedeza cuneata Using Response Surface Methodology", Kor. J. Food Cookery .Sci., 28(3), pp. 275-283, 2012. DOI: http://dx.doi.org/10.9724/kfcs.2012.28.3.275   DOI
28 J. G Lee, E. H. Kim, J. H. Lee, "Effect of Gentianae Radix on Neurogenesis and Apoptosis in Hippocampus of ethanol-induced Newborn Rats" J. Oriental Neuro. 21(2), pp. 29-44, 2010.
29 Min S. K. Modern psychiatry 5th ilchokak seoul pp. 32, 39-40, pp. 44-50, 2011.
30 J. Tuma, C. A.Casey, "Dangerous byproducts of alcohol breakdown focus on adducts" Alcohol Res Health. 27(4), pp. 285-290, 2013.
31 Y. S. Kim, J. Y. Park, Y. B. Kwon, D. W. Lim, M. K. Song , H. Y. Choi, H. C. Kim, "Hepatoprotective Effects of Hovenia dulcis Extract on Acute and Chronic Liver Injuries induced by Alcohol and Carbon Tetrachloride" J. Herb. 28(4), pp. 25-32, 2013. DOI: http://dx.doi.org/10.6116/kjh.2013.28.4.25
32 J. H. Lefkowitch "Morphology of alcoholic liver disease" Clin. Liver Dis. 9, pp. 37-53, 2005. DOI: http://dx.doi.org/10.1016/j.cld.2004.11.001   DOI
33 J. M. Crawford, "Histologic findings in alcoholic liver disease" Clin. Liver Dis. 16, pp. 699-716, 2012. DOI: http://dx.doi.org/10.1016/j.cld.2012.08.004   DOI
34 K. A. Chung, M. J. Cheong "Effects of Lespedeza Caneata ethanol extract on the Liver, Kidneys of Lead administered Mice" JKAIS, vol. 17, no. 6, pp. 207-214, 2016. DOI: http://dx.doi.org/10.5762/KAIS.2016.17.6.207
35 J. S. Kim, "the antioxidant enzymes and histopathological changes of liver and kidney according to administered dosage of Bambusae caulis in liquamen in mice" Kor. J. Elec. Microscop. 32(4), pp. 399-410, 2002.
36 M. Koneru, B. D. Sahu, J. M. Kumar, M. Kuncha, A. Kadari, E. K. Kilari, R. Sistla, "Fistin protects liver from binge alcohol-induced roxicity by mechanisms incuding inhibition of matrix metalloproteins and oxidative stress" J. funct. food 22, pp. 588-601, 2016.   DOI
37 D. H. Kim, Y. K. Deung, Y. M. Lee, Y. S. Yoon, K. J. Lee, "The effect of saengshik od the hepatotoxicity of mouse induced by carbon tetrachloride" Kor. J. Elec. Microscop, 37(1), pp. 11-21, 2007.