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http://dx.doi.org/10.5762/KAIS.2015.16.3.1964

Functional expression of TREK1 channel in human bone marrow and human umbilical cord vein-derived mesenchymal stem cells  

Park, Kyoung Sun (Division of Molecular and Life Sciences, Hanyang University)
Kim, Yangmi (Department of Physiology, College of Medicine, Chungbuk National University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.16, no.3, 2015 , pp. 1964-1971 More about this Journal
Abstract
Human bone marrow or human umbilical cord vein derived-mesenchymal stem cells (hBM-MSCs or hUC-MSCs) have known as a potentially useful cell type for clinical therapeutic applications. We investigated two-pore domain potassium (K2P) channels in these cells. K2P channels play a major role in setting the resting membrane potential in many cell types. Among them, TREK1 is targets of hydrogen, hypoxia, polyunsaturated fatty acids, antidepressant, and neurotransmitters. We investigated whether hBM-MSCs and hUC-MSCs express functional TREK1 channel using RT-PCR analysis and patch clamp technique. Potassium channel with a single channel conductance of 100 pS was found in hUC-MSCs and BM-MSCs and the channel was activated by membrane stretch (-5 mmHg ~ -15 mmHg), arachidonic acid ($10{\mu}M$) and intracellular acidosis (pH 6.0). These electrophysiological properties were similar to those of TREK1. Our results suggest that TREK1 is functionally present in hBM-MSCs and hUC-MSCs, where they contribute to its resting membrane potential.
Keywords
human bone marrow-derived mesenchymal stem cells; human umbilical cord vein-derived mesenchymal stem cells; K2P channel; single channel recording; TREK1;
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