Browse > Article
http://dx.doi.org/10.5762/KAIS.2015.16.1.585

Study of the enzymatic action of the chymopapain using pluronic based nano-carrier system on the cadaveric nucleus pulposus tissue  

Choi, Won Il (Life Science Research Institute, Daewoong Pharm)
Tae, Gi Yoong (School of Materials Science and Engineering, Gwangju Institute of Science and Technology)
Hong, Young Ki (Department of Sports Medicine, Cheongju University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.16, no.1, 2015 , pp. 585-592 More about this Journal
Abstract
The objective of this study is to determine if when chymopapain is loaded onto a nano-carrier, an injection of it reduces the spreading range of the drug within the discs. The materials for the experiment, which were conducted for three weeks, included fifteen intervertebral discs taken from two cadavers, which were divided according to the types of injected chymopapain solutions as follows: ordinary chymopapain group and nano-carrier system group. The nano-carrier system group was again divided into two subgroups according to the types of pluronics, the basic material for the nano-carriers: Pluronic F 127(DA-PF 127) in the nano-carrier group and Pluronic F 68(DA-PF 68) in the nano-carrier group. The results showed that the action of chymopapain using a pluronic-based nano-carrier system was localized around the center of the injection site instead of broad spreading, compared to that of the ordinary chymopapain group (p<0.01). This characteristic suggests a possible application to effective agents for minimally invasive spinal treatment through which disc lesions were removed selectively.
Keywords
chymopapain; intervertebral disc herniation; minimally invasive spinal treatment;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 P.Linse,M.Malmsten,Temperature-dependent micellization inaqueous block copolymer solutions. Macromolecules. 25 pp.5434-5439. 1992. DOI: http://dx.doi.org/10.1021/ma00046a048   DOI
2 R.Nagarajan,Solubilization of hydrocarbons and resulting aggregate shape transitions inaqueous solutions of Pluronic (PEO-PPO-PEO) block copolymers, Colloids SurfacesB. Bio interfaces 16 pp.55-72. 1999. DOI: http://dx.doi.org/10.1016/S0927-7765(99)00061-2   DOI
3 Wu Z, Wei LX, Li J, Wang Y, Ni D, Yang P, Zhang Y. Percutaneous treatment of non-contained lumbar disc herniation by injection of oxygen-ozone combined with collagenase. Eur J Radiol. 72(3) pp.499-504. 2009. DOI: http://dx.doi.org/10.1016/j.ejrad.2008.07.029   DOI
4 Riquelme C, Musacchio M, Mont'Alverne F, Tournade A. Chemonucleolysis of lumbar disc herniation with ethanol. J. Neuroradiol. 28(4) pp.219-29. 2001.
5 Adam D, Pevzner E, Gepstein R. Comparison of percutaneous nucleoplasty and open discectomy in patients with lumbar disc protrusions. Chirurgia (Bucur). 108(1): pp. 94-8. 2013.
6 Laxmaiah Manchikanti, Vijay Singh, Frank J.E. Falco, Aaron K. Calodney, Obi Onyewu, Standiford Helm II, Ramsin M. Benyamin, Joshua A. Hirsch. An Updated Review of Automated Percutaneous Mechanical Lumbar Discectomy for the Contained Herniated Lumbar Disc, Pain Physician, 16 p.151-84 2013.
7 Singh V, Manchikanti L, Calodney AK, Staats PS, Falco FJ, Caraway DL, Hirsch JA, Cohen SP. Percutaneous lumbar laser disc decompression: an update of current evidence. Pain Physician. 16(2) pp. 229-60. 2013.
8 Deyo RA. Chymopapain for herniated interverte bral disc. A methodologic analysis and an agenda for future research. Spine. 9(5) pp.474-8. 1964. DOI: http://dx.doi.org/10.1097/00007632-198407000-00010   DOI
9 Ogbonnaya S, Kaliaperumal C, Qassim A, O'Sullivan M. Outcome of nucleoplasty in patients with radicular pain due to lumbar intervertebral disc herniation. J Nat Sci Biol Med. 4(1) pp.187-90. 2013. DOI: http://dx.doi.org/10.4103/0976-9668.107288   DOI
10 Wardlaw D, Rithchie IK, Sabboubeh AF, Vavdha M, Downing M, Eastmond CJ. Prospective randomized trial of chemonucleolysis compared with surgery for soft disc herniation with 1-year, intermediate, and long-term outcome: part II: the radiological outcome. Spine. 1;38(17) pp.1058-64. 2013.   DOI
11 Garvin PJ, Jennings RB, Stern IJ. Enzymatic digestion of the nucleus pulposus: a review of experimental studies with chymopapain. The Orthopedic clinics of North America. 8(1) pp.27-35. 1977.
12 Smith L. Enzyme Dissolution of the Nucleus Pulposus in Humans. Jama 187 pp.137-40. 1964. DOI: http://dx.doi.org/10.1001/jama.1964.03060150061016
13 Chen YC, Lee SH, Saenz Y, Lehman NL. Histologic findings of disc, end plate and neural elements after coblation of nucleus pulposus: an experimental nucleoplasty study. Spine J. 3(6) pp.466-70. 2003. DOI: http://dx.doi.org/10.1016/S1529-9430(03)00143-8   DOI
14 Castro WH, Halm H, Jerosch J, Steinbeck J, Meyer M, Gohlke KH et al. Long-term changes in the magnetic resonance image after chemo nucleolysis. Eur Spine J. 3(4) pp.222-4. 1994. DOI: http://dx.doi.org/10.1007/BF02221597   DOI
15 Sharps LS, Isaac Z. Percutaneous disc decom pression using nucleoplasty. Pain Physician. 5(2) pp.121-6. 2002.
16 Choi WI, Kim YH, Tae GY. Controlled release of proteins from Pluronic-based nano-carrier. Macromolecular Research. 19(6) pp.639-642. 2011. DOI: http://dx.doi.org/10.1007/s13233-011-0602-9   DOI
17 Hong YK, Derby R, Wolfer LR, Kim SU, Kang BS, Kim NH, Yoo SH, Lee SJ, Lee SH. An assessment of a new navigatable percutaneous disc decompression device (l'DISQ) through histologic evaluation and thermo-mapping in human cadaveric discs. Pain Medicine. 13(8) pp. 1000-3. 2012. DOI: http://dx.doi.org/10.1111/j.1526-4637.2012.01447.x   DOI
18 Lim JH, Lee HJ, Lee SH. Application of percutaneous cervical nucleoplasty using the navigable disc decompression device in patient of cervical herniated intervertebral disc: a case report. Ann Rehabil Med. 37(5) pp. 730-4. 2013. DOI: http://dx.doi.org/10.5535/arm.2013.37.5.730   DOI
19 Choi WI, Tae GY, Kim YH. One pot, single phase synthesis of thermo-sensitive nano-carriers by photocrosslinking of a diacrylated pluronic. J. Mater. Chem. 18 pp.2769-2774. 2008. DOI: http://dx.doi.org/10.1039/b801262h   DOI
20 Choi WI, Kim JY, Kang C, Byeon CC, Kim YH, Tae GY. Tumor regression in vivo by photothermal therapy based on gold-nanorod-loaded, functional nanocarriers. ACS Nano. 5(3) pp.1995-2003. 2011. DOI: http://dx.doi.org/10.1021/nn103047r   DOI   ScienceOn
21 Gotfried Y, Bradford DS, Oegema TR, Jr. Facet joint changes after chemonucleolysis-induced disc space narrowing. Spine. 11(9) pp. 944-50. 1986. DOI: http://dx.doi.org/10.1097/00007632-198611000-00016   DOI
22 Dunlop RB, Adams MA, Hutton WC. Disc space narrowing and the lumbar facet joints. J Bone Joint Surg Br. 66(5) pp.706-10. 1984.
23 Castro WH, Halm H, Jerosch J, Steinbeck J, Meyer M, Gohlke KH et al. Long-term changes in the magnetic resonance image after chemonucleolysis. Eur Spine J. 3(4) pp.222-4. 1994. DOI: http://dx.doi.org/10.1007/BF02221597   DOI