Browse > Article
http://dx.doi.org/10.5762/KAIS.2015.16.1.365

Effect of GM-CSF on Porcine Parthenotes Development  

Lee, Jae-Dal (Department of Pet Care, Hyejeon College)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.16, no.1, 2015 , pp. 365-370 More about this Journal
Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important hematopoietic growth factor and immune modulator. The aim of this study was to evaluate the effects of GM-CSF on the development and cell number of porcine parthenotes, as well as on their expression of implantation-related genes. In the present study, porcine parthenogenatic activated embryos were cultured in a protein-free culture medium in the absence or presence of 5, 10 and 20 ng/ml GM-CSF for 7 days. The percentage of blastocyst formation, total cell number and gene expressions were evaluated. The results showed that the addition of 20 ng/ml GM-CSF to protein-free culture medium significantly increased the blastocoel formation ($26.14{\pm}2.03%$ vs. $3.55{\pm}0.51%$, p < 0.05). In addition, the cell number also increased when they were cultured in the presence of 20 ng/ml GM-CSF ($43.51{\pm}3.6%$ vs. $30.68{\pm}5.51%$, p < 0.05). A real time reverse transcripts polymerase chain reaction (RT-PCR) showed that GM-CSF enhances mRNA expression of the interleukin-6, but does not influence the leukemia inhibitory factor (LIF) receptor mRNA expression in blastocyst stage parthenotes. These results suggest that GM-CSF may enhance the viability of porcine embryos developing in vitro in a defined culture medium.
Keywords
Porcine parthenotes Development; GM-CSF; Gene expression; RT-PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 van Montfort, T., et al., A chimeric HIV-1 envelope glycoprotein trimer with an embedded granulocytemacrophage colony-stimulating factor (GM-CSF) domain induces enhanced antibody and T cell responses. J Biol Chem, 286(25): p. 2250-61. 2011 DOI: http://dx.doi.org/10.1074/jbc.M111.229625
2 Shi, Y., et al., Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don't know. Cell Res, 16(2): p. 126-33. 2006. DOI: http://dx.doi.org/10.1038/sj.cr.7310017   DOI
3 de Moraes, A.A. and P.J. Hansen, Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos. Biol Reprod, 57(5): p. 1060-5. 1997 DOI: http://dx.doi.org/10.1095/biolreprod57.5.1060   DOI
4 Imakawa, K., et al., A novel role for GM-CSF: enhancement of pregnancy specific interferon production, ovine trophoblast protein-1. Endocrinology, 132(4): p. 1869-71. 1993 DOI: http://dx.doi.org/10.1095/biolreprod57.5.1060   DOI
5 Robertson, S.A., GM-CSF regulation of embryo development and pregnancy. Cytokine Growth Factor Rev, 18 (3-4): p. 287-98. 2007 DOI: http://dx.doi.org/10.1016/j.cytogfr.2007.04.008   DOI   ScienceOn
6 Ziebe, S., et al., A randomized clinical trial to evaluate the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) in embryo culture medium for in vitro fertilization. Fertil Steril, 99 (6): p. 1600-9, 2013 DOI: http://dx.doi.org/10.1016/j.fertnstert.2012.12.043   DOI
7 Hardy,k.,A.H. Handyside, and R.M. Winston, The human blastocyst; cell number, deat and allocation during late preimplantation development in vitro. dev, 107(3): p597-604.1989
8 Wang, W.H. and B.N. Day, Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione. Zygote, 10(2): p. 109-15, 2002 DOI: http://dx.doi.org/10.1017/S0967199402002150
9 Stewart, C.L. and E.B. Cullinan, Preimplantation development of the mammalian embryo and its regulation by growth factors. Dev Genet, 21(1): p. 91-101, 1997 DOI: http://dx.doi.org/10.1002/(SICI)1520-6408(1997)21:1<91::AID-DVG11>3.0.CO;2-D   DOI
10 Kim DH, et al., Effect of GM-CSF on the embryonic development and the expression of implantation related genes of mouse embryos. . Kor J Fertil Steril, . 2002 (29): p. 83-90, 2002   과학기술학회마을
11 Steel, R.G.D. and J.H. Torrie, Principles and Procedures of Statistics, NY: Mcgraw Hill Book co .2000
12 Ruef, C .and D .L. Coleman, colony - stimulating factor: pleiotropic cytokine with potential clinical usefulness. Rev Infect Dis, 12(1): p. 41-62,2006 DOI: http://dx.doi.org/10.1093/clinids/12.1.41
13 Elaimi, A., et al., The effect of GM-CSF on development and aneuploidy in murine blastocysts. Hum Reprod, 27(6): p. 1590-5,2012 DOI: http://dx.doi.org/10.1093/humrep/des108   DOI
14 Watson, A.J., The cell biology of blastocyst development. Mol Reprod Dev,. 33 (4): p. 492-504,2006 DOI: http://dx.doi.org/10.1002/mrd.1080330417   DOI
15 Hernandez-Ledezma, J.J., et al., Expression of bovine trophoblast interferon in conceptuses derived by in vitro techniques. Biol Reprod, 47(3): p. 374-80,1992 DOI: http://dx.doi.org/10.1095/biolreprod47.3.374   DOI
16 Desai, N., et al., Evaluation of the effect of interleukin-6 and human extracellullar matrix on embryonic development. Hum Reprod, 14(6): p. 1588-92,1999 DOI: http://dx.doi.org/10.1093/humrep/14.6.1588   DOI
17 Conquet, F. and P. Brulet, Developmental expression of myeloid leukemia inhibitory factor gene in preimplantation blastocysts and in extraembryonic tissue of mouse embryos. Mol Cell Biol, 10(7): p. 3801-5,2007   DOI