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http://dx.doi.org/10.5762/KAIS.2014.15.8.5412

Expression of the red sea bream iridovirus (RSIV) capsid protein using a yeast surface display method  

Suh, Sung-Suk (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Park, Mirye (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Hwang, Jinik (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.15, no.8, 2014 , pp. 5412-5418 More about this Journal
Abstract
The red seabream iridovirus (RSIV), which belongs to the iridoviridae, causes infectious fish diseases in many Asian countries, leading to considerable economic losses to the aquaculture industry. Using the yeast surface display (YSD) technique, a new experimental system was recently developed for the detection and identification of a variety of marine viruses. In this study, a coat protein gene of RSIV was synthesized based on the nucleotide sequence database and subcloned into the yeast expression vector, pCTCON2. The expression of viral coat proteins in the yeast strain, EBY100, was detected by flow cytometry and Western blot analysis. Finally, they were isolated from the yeast surface through a treatment with ${\beta}$-mercaptoethanol. The data suggests that the YSD system can be a useful method for acquiring coating proteins of marine viruses.
Keywords
Capsid protein; Flow cytometry; Recombinant protein purification; RSIV; Yeast surface display;
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