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http://dx.doi.org/10.5762/KAIS.2014.15.11.6774

Transcriptome Analysis of Human HaCaT Keratinicytes by Ginsenosides Rb1 and Rg1  

Kim, Jung Min (NAR Center, Inc., Daejeon Oriental Hospital of Daejeon University)
Cho, Won June (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Yoon, Hee Seung (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Bang, In Seok (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.15, no.11, 2014 , pp. 6774-6781 More about this Journal
Abstract
This study examined the efficacy and the mechanism of action of biological response modifiers, ginsenosides Rb1 and Rg1 isolated from Panax ginseng C.A. Meyer on human keratinocytes HaCaT cell lines. A non-significant cytotoxic response was obtained in the HaCaT cell lines on treatment with various concentrations of ginsenosides Rb1 and Rg1 for different time durations. Furthermore, the global changes in the mRNA profile of HaCaT cells were investigated using DNA microarrays after stimulation with the ginsenosides Rb1 and Rg1. Ginsenosides Rb1 and Rg1 strongly increased FGF2 in HaCaT cells, and were found to be a candidate gene for antioxidant activity and elasticity. Other key candidate genes for antioxidant activity, such as FANCD2, LEPR, and FAS, also show enhanced regulation in HaCaT cells treated with ginsenoside Rb1. This study will be useful for understanding the regulatory genes involved in skin elasticity and signal transduction pathway stimulated by the ginsenoside Rb1. This paper currently focuses on the key factors regulating the interaction of anti-aging principles and skin elasticity.
Keywords
Ginsenoside Rb1; Rg1; Human keratinocyte HaCaT cell line; Microarray analysis;
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