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http://dx.doi.org/10.5762/KAIS.2013.14.8.4086

Expression of Mycosporine-like Amino Acids Biosynthetic Genes in the Chlamydomonas sp. Exposed to Radiofrequency  

Hwang, Jinik (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
Chang, Man (Marine Ecosystem Research Division, Korea Institute of Ocean Science & Technology)
Lee, Gunsup (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Lee, Juyun (Marine Ecosystem Research Division, Korea Institute of Ocean Science & Technology)
Kim, Donggiun (Department of Biological Science, Silla University)
Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.14, no.8, 2013 , pp. 4086-4092 More about this Journal
Abstract
Mycosporine-like amino acids (MAAs) are UV-absorbing substances, and diverse marine organisms have the evolved the capacity to diminished the direct and indirect damaging effects of environmental ultraviolet radiation by synthesis and accumulation of MAAs. In this study, we manufactured a radiofrequency (RF) generation device and applied to microalgal culture. $0.35{\pm}0.05$ mHz of RF was supplied to culture vessel for Chlamydomonas sp. and samples were harvested at the designated time intervals (1, 0.5, 1 and 2 hr). MAAs biosynthetic genes, dehydroquinate synthase homolog (DHQS-like) and nonribosomal peptide synthetase homolog (NRPS-like), were cloned from Chlamydomonas sp. and their gene expressions under the RF exposure were analyzed using qRT-PCR. DHQS-like and NRPS-like gene expressions of Chlamydomonas sp. exposed to RF were increased 1.46 and 1.19 fold at 1 hr, respectively. These results means that DHQS-like and NRPS-like genes can be good biomarker candidates for diagnosis of MAAs biosynthesis in the Chlamydomonas sp.
Keywords
Chlamydomonas sp.; DHQS-like; MAAs; NRPS-like; Radiofrequency;
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