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http://dx.doi.org/10.5762/KAIS.2013.14.8.3843

Effect of Apoptosis on Porcine Parthenotes Development in vitro  

Lee, Jae-Dal (Department of Pet Care, Hyejeon College)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.14, no.8, 2013 , pp. 3843-3849 More about this Journal
Abstract
This study was conducted to determine the effects of fetal bovine serum (FBS), bovine serum albumin (BSA) and epidermal growth factor (EGF) on blastocoel formation, total cell number, apoptosis and apoptosis-related gene expression of porcine diploid parthenotes developing in vitro. The addition of 0.4% BSA to the culture medium enhanced the development of 2-cell stage parthenotes to the blastocysts stage (p<0.01). Treatment of FBS reduced cell numbers of blastocysts (p<0.01) and increased the percentage of apoptosis in blastocysts (p<0.001). However, BSA increased cell numbers, only in the presence of EGF reverse-transcriptase polymerase chain reaction revealed that EGF enhanced the mRNA expression of Bcl-xL in the presence of 0.4% BSA but BSA and EGF alone had no effect. However, Treatment of FBS reduced Bcl-xL mRNA expression (p<0.05) and enhanced Bak expression. This result suggests that apoptosis related gene expression is significantly affected by supplements, which may result in alteration of apoptosis and embryo viability of porcine embryos developing in vitro.
Keywords
Apoptosis; Blastocyst; BSA; EGF; FBS; Porcine embryo;
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