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http://dx.doi.org/10.5762/KAIS.2013.14.3.1191

Genotypic Detection of Extended-Spectrum β-Lactamase-Producing of Klebsiella pneumoniae  

Yook, Keun-Dol (Dept. of Clinical Laboratory Science, Deajeon Health Science College)
Yang, Byoung-Seon (Dept. of Clinical Pathology, Jinju Health College)
Park, Jin-Sook (Dept. of Biotechnology, Hannam University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.14, no.3, 2013 , pp. 1191-1196 More about this Journal
Abstract
Among Gram-negative pathogens in Korea, the incidence of resistance to third generation cephalosporins is becoming an ever-increasing problem. The production of extended-spectrum ${\beta}$-lactamase (ESBL) is the main mechanism of bacterial resistance to a third-generation cephalosporins and monobactams. Accurate identification of the ESBL genes are necessary for surveillance and epidemiological studies of the mode of transmission in the hospital. This study was conducted to detect the genes encoding ESBL of 46 K. penumoniae isolated from Daejeon, Chungnam and Chungbuk regional university hospitals from February to August in 2012. The phenotypes of the isolated specimens were examined according to the combination disc test (CDT) by the Clinical and Laboratory Standards Institute (CLSI). Forty two ESBL producing K. penumoniae isolates could be detected using ceftazidime (CAZ) discs with and without clavulanate (CLA). By CDT, 42 K. pneumoniae strains were confirmed to be ESBL strains. Genotyping was performed by multiplex PCR with type-specific primers. By PCR analysis, TEM gene in 46 strains, SHV gene in 37 strains and CTX-M genes in 14 strains were identified. Ten isolates did carry genes encoding ESBLs of all types TEM, SHV and CTX-M. The multiplex polymerase chain reaction (PCR) analysis was better to detect and differentiate ESBL producing K. penumoniae strains in clinical isolates.
Keywords
Extended-spectrum ${\beta}$-lactamases; Klebsiella penumoniae; Polymerase chain reaction;
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1 Monica C, Sonia MT, Alejandro P, Angeles BN, David D, Francisca V, Rosa M, German V. Risk factors for colonization and infection in a hospital outbreak caused by a strain of Klebsiella pneumoniae with reduced susceptibility to expanded-spectrum cephalosporins. J Clin Microbiol, 42:42-49, 2004.
2 Pnaiara E, Platouka H, Dimopoulou E, Tzelepi B, Miriagou, Tzouvelekis LS. Diversity of beta-lactam resistance levels among related Klebsiella pneumoniae strains isolated in an intensive care unit. J Chemother, 12:204-207, 2000.   DOI   ScienceOn
3 Silva J, Gatica R, Aguilar C, Becerra Z, Garza-Ramos U, Velazquez M, Miranda G, Leanos B, Solorzano F, Echaniz G. Outbreak of infection with extended-spectrum beta-lactamase producing Klebsiella pneumoniae in a Mexican hospital. J Clin Microbiol, 39:3193-3196, 2001. DOI: http://dx.doi.org/10.1128/JCM.39.9.3193-3196.2001   DOI   ScienceOn
4 Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing. Sixteenth informational supplement. CLSI document M100-S9, Wayne, PA. 2001.
5 Bedenic B, Randegger C, Boras A, Haechler H. Comparison of five different methods for detection of SHV extened-spectrum beta-lactamases. J Chemother, 13:24-33, 2001.   DOI   ScienceOn
6 Chin Fu Lin, Shin Kuang Hsu, Chao Hsien Chen, Jr Rung Huang, Genotypic detection and molecular epidemiology of extended-spectrum $\beta$-lactamase-producing Escherichia coli and Klebsiella pneumoniae in a regional hospital in central Taiwan. J Med Microbiol, 59:665-671, 2010. DOI: http://dx.doi.org/10.1099/jmm.0.015818-0   DOI   ScienceOn
7 Bradford PA. Extended-spectrum $\beta$-lactamase in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev, 14:933-951, 2001.   DOI   ScienceOn
8 Son SH, Lee DJ, Kim CG, Kim JM, Bae HJ. Distribution TEM, SHV type beta-lactamase gene of Escherichia coli and Klebsiella pneumoniae. Infection, 29:271-276, 1997.
9 Jacoby GA, Medeiros AA. More extend-spectrumlatamases. Antimicrob Agents Chemother, 35:1697-1704, 1991.   DOI   ScienceOn
10 Heritage JP, Hawkey M, Todd NI, Lewis J. Transposition of the gene encoding a TEM-12 extend-spectrum-latamases. Antimicrob Agents Chemother, 36: 1981-1986, 1992. DOI: http://dx.doi.org/10.1128/AAC.36.9.1981   DOI   ScienceOn
11 Kil KS, Darouiche RO, Hull RA, Mansouri MD, Musher DM. Identification of a Klebsiella pneumoniae strain associated with nosocomial urinary tract infection. J Clin Microbiol, 35:2370-2374, 1997.
12 Bingen EH, Desjardins P, Arlet G, Bourgeois F, Mariani-Kurkdjian P, Lambert-Zechovsky NY, Denamur E, Philippon A, Elion J. Molecular epidemiology of plasmid spread among extended broad-spectrum beta-lactamase producing Klebsiella pneumoniae isolates in a pediatric hospital. J Clin Microbiol, 31:179-184, 1993.
13 Galani I, Russell EG, Tymms M, Roper EJ, Grayson ML, Turnidge J. Transferable plasmid mediating resistance to multiple antimicrobial agents in Klebsiella pneumoniae isolates in Greece. Clin Microbiol Infect, 8:579-588, 2002. DOI: http://dx.doi.org/10.1046/j.1469-0691.2002.00391.x   DOI   ScienceOn
14 Gazouli M, Kaufmann ME, Tzelepi E, Dimopoulou H, Paniara O, Tzouvelekis LS. Study of an outbreak of cefoxitin-resistance Klebsiella pneumoniae in a general hospital. J Clin Microbiol, 35:508-510, 1997.
15 Macrae MB, Shannon KP, Rayner DM, Kaiser AM, Hoffman PN, French GL. A simultaneous outbreak on a neonatal unit of two strains of multiply antibioticresistant Klebsiella pneumoniae controllable only by ward closure. J Hosp Infect, 49:183-192, 2001. DOI: http://dx.doi.org/10.1053/jhin.2001.1066   DOI   ScienceOn