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http://dx.doi.org/10.5762/KAIS.2012.13.8.3532

Effect of maternal gene expression on porcine oocytes in vitro maturation  

Lee, Jae-Dal (Department of Pet Care, Hyejeon College)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.13, no.8, 2012 , pp. 3532-3536 More about this Journal
Abstract
Understanding of the maternal transcriptome increased to elucidate the underlying molecular mechanism of normal oocyte maturation, which depends on a precise sequence of changes in maternal genes expression. Previous reports that the translational potential of a maternal mRNA is generally determined by the length of the poly(A) tail, and deadenylation is usually the first sign of mRNA degradation. However, in vitro cultured system has the underlying molecular mechanisms remain unclear. We determined whether the role of molecular basis, four important maternal genes, C-mos, cyclin-B1 (regulatory subunit of MPF), BMP15 and GDF9, were selected for detection of their precise mRNA expression patterns by real-time PCR and for determination of their polyadenylation status by poly(A) tail PCR during oocyte maturation. In the present study. the abnormal expression of maternal mRNAs prior to zygotic genome activation, which results in suppression of the corresponding protein level, may be responsible for, at least in part, a profound defect in further embryonic development. Reasonable expression of maternal gene is crucial for proper oocyte maturation and further embryonic development.
Keywords
Porcine oocytes; Maternal gene; Polyadenylation; In vitro maturation;
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1 Bettegowda A, Smith G W. Mechanisms of maternal mRNA regulation: implications for mammalian early embryonic development. Front Biosci 12:3713-3726, 2007.   DOI
2 Juge F, Zaessinger S, Temme C, Wahle E, Simonelig M. Control of poly(A) polymerase level is essential to cytoplasmic polyadenylation and early development in Drosophila. EMBO J 21:6603-6613, 2002.   DOI
3 Hashiba Y, Asada Y, Heikinheimo O, Lanzendorf SE, Mizutani S. Microinjection of antisense c-mos oligonucleotides prevents the progression of meiosis in human and hamster oocytes. Fertil Steril 76:143-147, 2001.   DOI
4 O'Keefe SJ, Wolfes H, Kiessling AA, Cooper GM. Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg. Proc Natl Acad Sci 86:7038-7042, 1989.   DOI
5 Ohashi S, Naito K, Sugiura K, Iwamori N, Goto S, Naruoka H, Tojo H. Analyses of mitogen-activated protein kinase function in the maturation of porcine oocytes. Biol Reprod 68:604-609, 2003.   DOI
6 Newman B, Dai Y. Transcription of c-mos protooncogene in the pig involves both tissue-specific promoters and alternative polyadenylation sites. Mol Reprod Dev 44:275-288, 1996.   DOI
7 Tay J, Hodgman R, Richter JD. The control of cyclin B1 mRNA translation during mouse oocyte maturation. Dev Biol 221:1-9, 2000.   DOI
8 Tremblay K, Vigneault C, McGraw S, Sirard MA. Expression of cyclin B1 messenger RNA isoforms and initiation of cytoplasmic polyadenylation in the bovine oocyte. Biol Reprod 72: 1037-1044, 2005.   DOI
9 Juengel JL, Bodensteiner KJ, Heath DA, Hudson NL, Moeller CL, Smith P, Galloway SM, Davis GH, Sawyer HR, McNatty KP. Physiology of GDF9 and BMP15 signalling molecules. Anim Reprod Sci 82-83:447-460, 2004.   DOI   ScienceOn
10 McNatty KP, Juengel JL, Reader KL, Lun S, Myllymaa S, Lawrence SB, Western A, Meerasahib MF, Mottershead DG, Groome NP, Ritvos O, Laitinen MP. Bone morphogenetic protein 15. and growth differentiation factor 9 co-operate to regulate granulose cell function in ruminants. Reproduction 129:481-487, 2005   DOI