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http://dx.doi.org/10.5762/KAIS.2012.13.12.6196

Extraction of anti-microalgal material from Laminaria spp. and effect of oligo-alginate derivatives on membrane potential  

Lee, Gunsup (Korea Institute Ocean Science & Technology)
Chang, Man (Korea Institute Ocean Science & Technology)
Shin, Kyoungsoon (Korea Institute Ocean Science & Technology)
Kim, Donggiun (Department of Biological Science, Silla University)
Auh, Chung-Kyoon (Department of Biological Science, Mokpo National University)
Lee, Taek-Kyun (Korea Institute Ocean Science & Technology)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.13, no.12, 2012 , pp. 6196-6202 More about this Journal
Abstract
Oligo-alginate derivatives were extracted from brown algae and its antimicroalgal effects and reaction mechanism were investigated. Oligo-alginate derivatives were produced from sequential hydrolysis of high molecular weight alginate by treatment of 2 N HCl and 1% $H_2O_2$. Antimicroalgal activity of extracts was proportional to reaction time and activity was highest at 4 hrs. When oligo-alginate derivatives were treated to Akashiwo sanguinea and Cochlodinium polykrikoides, mobilities of cells were ceased. A. sanguinea cells were crushed and plasmolysis was induced in C. polykrikoides cells. To investigate the action mechanism of oligo-alginate derivatives, changes of intracellular (pHi) and extracellular pH (pHe) were determined in the microalgal cells exposed to 0.05% of oligo-alginate derivatives. pHi was decreased about 0.3 unit and pHe was increased about 0.9 unit. These results suggested that change of membrane potential by oligo-alginate derivatives could led to microalgal cell death.
Keywords
Lamminaria sp.; Alginate; Akashiwo sanguinea; C. polykrikoides; Cell death;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 H.G. Kim, S.G. Lee and K.H. An. "Recent red tides in Korean coastal waters." Nat Fish. Res. Develop. Ins. Korea, 280p. 1997.
2 D.W. Renn. "Medical and biotechnological applications of marine macroalgal polysaccharides." Marine Biotechnology vol. 1: Pharmaceutical and bioactive natural products, ed. D.H. Attaway and O.R. Zaborsky. Plenum Press, New York. pp181-196, 1993.
3 N.H. Jeoung, H.J. Son and S.Y. Jeong. "The Algicidal Activity of Pseudoalteromonas sp. NH-12 against the Toxic Dinoflagellate Alexandrium catenella." Kor. Environ. Agricul. 31(2), 175-184, 2012, Article(CrossRefLink)   과학기술학회마을   DOI
4 G.-H. Na, W.-J. Choi and Y.Y. Chun. "A study on red tide control with loess suspension." J. Aquacul. 9(3), 239-245, 1996.
5 S.H. Baek, H.H. Shin, M.-C. Jang, S.W. Kim, M. Son, H. Cho, and Y.O. Kim. "Algicidal Effects of a Newly Developed Thiazolidinedione Derivative, TD49, on Dinoflagellate Akashiwo sanguinea.". Ocean Polar Resear. 34(2):125-135, 2012, Article(CrossRefLink)   DOI
6 S.-Y. Jeong, Y.-T. Park and W.-J. Lee. "Isolation of marine bacteria killing red tide microalgae. III. Algicidal effects of marine bacterium, Micrococcus sp. LG-5 against the harmful dinoflagellate, Cochlodinium polykrikoides." J. Korean Fish. Soc. 33(4), 331-338, 2000.   과학기술학회마을
7 S.-C. Chae, Y.-N Jang and I,-K. Bae. "Development of anti-red tide material by activating red-mud." J. Miner. Soc. Korea, 18(4), 267-276, 2005.   과학기술학회마을
8 D. Renn. "Biotechnology and the red seaweed polysaccharide industry: status, needs and prospects." Trend. Biotechnol. 15(1), 9-14, 1997, Article(CrossRefLink)   DOI   ScienceOn
9 D.W. Renn. "Seaweeds and biotechnology- inseparable companions." Hydrobiol. 204/205, 7-13, 1990, Article(CrossRefLink)   DOI
10 R.J. Radmer. "Algal diversity and commercial algal products." Bioscience 46, 263-270, 1996, Article(CrossRefLink)   DOI
11 N.L. Thakur and A.N. Thakur. "Marine biotechnology: an overview." Ind. J. Biotechnol. 5, 263-268, 2006.
12 H. Ertesvag and S. Valla, "Biosynthesis and application of alginates." Polymer Degradation Solubility 59, 85-91, 1998, Article(CrossRefLink)   DOI   ScienceOn
13 B.H.A. Rehm and S. Valla, "Bacterial alginates: biosynthesis and application." Appl. Microbiol. Biotechnol. 48: 281-288, 1997, Article(CrossRefLink)   DOI
14 A.M.S. Mayer, A. Diaz, A. Pesce, M. Criscuolo, J.F. Groisman and R.M. de Lederkremer, "Biological activity in Macrocystis pyrifera from Argentina: sodium alginate, fucoidan and laminaran. III. Antiviral activity." Hydrobiol. 151/152, 497-500, 1987, Article(CrossRefLink)   DOI
15 A.M.S. Mayer and B. Panick, "Antitumor evaluation of marine algae in Argentina." Hydrobiol. 116/117, 529-533, 1984, Article(CrossRefLink)
16 A. Haug and G Larsen. Quantitative determination of the uronic acid composition of alginates." Acta Chem. Scand. 16, 1908-1918, 1962, Article(CrossRefLink)   DOI
17 J. Moss and M. Vaughan. "Structure and Function of ARF Proteins: Activators of Cholera Toxin and Critical Components of Intracellular Vesicular Transport Processes." J Biol. Chem. 270(21), 12327-12330, 1995, Article(CrossRefLink)   DOI
18 A.L. Usov, M.I. Bilan and N.G. Klochkov, 1995. Polysaccharides of algae 48. Polysaccharide composition of several calcareous red algae: S.-YIsolation of alginate from Corallina pilulifera P. et R. (Rhodophyta, Carallinaceae). Bot. Mar. 38: 43-51, Article(CrossRefLink)
19 T.K. Lee, S.M. Lee and W.S. Lee, "Preferential use of acetate over glucose involves acetate-mediated inhibition of glucose uptake during diauxic growth of carrot cells." Plant Cell Physiol. 40(10), 1046-1052, 1999, Article(CrossRefLink)   DOI
20 M. Kikuyama, K. Kuchitsu and N. Shibuya. "Membrane depolarization induced by N-Acetylchitooligosaccharide elicitor in suspension- cultured rice cells." Plant Cell Physiol. 38, 902-909. 1997, Article(CrossRefLink)   DOI
21 M. Hofmann, R. Ehness, T.K. Lee and T. Roitsch, "Intracellular protons are not involved in elicitor dependent regulation of mRNAs for defense related enzymes in Chenopodium." J Plant Physiol. 155, 527-532, 1999, Article(CrossRefLink)   DOI