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http://dx.doi.org/10.5762/KAIS.2011.12.10.4378

Anti-oxidative Activities of Angelica dahurica Radix Ethanol Extract  

Kim, Pil-Sun (Dept. of Herbal Skin Care, Daegu Haany University)
Lee, Tae-Jong (Dept. of Pathology, Daegu Fatima Hospital)
Kim, Yang-Hee (Dept. of Health Education, Taegu Science University)
Kim, Jung-Si (Dept. of Beauty Stylist, Daegu Polytechnic College)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.12, no.10, 2011 , pp. 4378-4384 More about this Journal
Abstract
In order to evaluate the anti-oxidative capabilities of Angelica dahurica Radix ethanol extract (ADEE), we analyzed the contents of polyphenol and flavonoid compounds and the electron donating ability from ADEE. For animal experimentation, the test agent was topically applied to the artificial tanning spots which were induced by 1,500 mJ/$cm^2$ of ultraviolet B radiation on the backs of brown guinea pigs weighing approximately 450~500g. The test agent of $30{\mu}{\ell}$ was applied (6areas per group) twice a day, five days a week, for five weeks. On completion of the experiment, the animals were sacrificed under anesthetization, and the artificial tanning spots were obtained by biopsy punch and stained with H&E to observe the histological change in the epidermis and dermis. As a result, the contents of polyphenol and flavonoid compounds in ADEE were 20.7mg/g and 19.5mg/g respectively. As the for electron-donating capability of ADEE, it was observed that ADEE displays a dose-dependent antioxidative capacity of 14.8% and 19.8% at the concentration of 500 and 1000 ${\mu}g/m{\ell}$ respectively. Tissue staining with H&E revealed that the epidermis of the control group was slightly thicker than that of the other groups. However no inflammation or any other undesirable effect on the skin tissue due to ADEE was observed. These results indicate that ADEE is of value as a natural antioxidant.
Keywords
Angelica dahurica Radix; polyphenol; flavonoid; electron donating; H&E staining;
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